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Publications

Below is a list of available WFRC peer reviewed and published science.

Filter Total Items: 2525

Development of a biotinylated DNA probe for detection of infectious hematopoietic necrosis virus Development of a biotinylated DNA probe for detection of infectious hematopoietic necrosis virus

A nonrad~oact~ve DNA probe assay was developed to detect and ~dent~fy infect~ous hernatopoiet~c necrosls virus (IHNV) uslng a dot blot format The probe a synthet~c DNA oligonucleot~de labeled enzymatlcally w~th biotln hybnd~zed spec~f~cally w~th nucleocaps~d mRNA extracted from Infected cells early In the vlrus repl~cation cycle A rap~d guan~dln~um th~ocyanate based RNA extraction method...
Authors
R.E. Deering, C.K. Arakawa, K.H. Oshima, P.J. O’Hara, M.L. Landolt, J. R. Winton

Comparison of genome size and synthesis of structural proteins of Hirame Rhabdovirus, infectious hematopoietic necrosis virus, and viral hemorrhagic Septicemia virus Comparison of genome size and synthesis of structural proteins of Hirame Rhabdovirus, infectious hematopoietic necrosis virus, and viral hemorrhagic Septicemia virus

Genomic RNA was extracted from purified virions of hirame rhabdovirus (HRV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The full-length RNA was analyzed using formaldehyde agarose gel electrophoresis followed by ethidium bromide staining. Compared with an internal RNA size standard, all three viral genomic RNAs appeared to have...
Authors
Toyohiko Nishizawa, Mamoru Yoshimizu, James R. Winton, Takahisa Kimura

Recent advances in detection and control of infectious hematopoietic necrosis virus in aquaculture Recent advances in detection and control of infectious hematopoietic necrosis virus in aquaculture

Infectious hematopoietic necrosis (IHN) is one of the most important viral diseases of salmon and trout reared in culture. The disease remains untreatable with avoidance being the only control measure. Much has been learned about the chemical, physical, and serological characteristics of the rhabdovirus causing IHN, but critical gaps exist in our understanding of the biology of the virus...
Authors
James R. Winton

Brood stock segregation of spring chinook salmon Oncorhynchus tshawytscha by use of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody technique (FAT) affects the prevalence and levels of Renibacterium salmoninarum in Brood stock segregation of spring chinook salmon Oncorhynchus tshawytscha by use of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody technique (FAT) affects the prevalence and levels of Renibacterium salmoninarum in

A study of the effect of maternal Renibacterium salmoninarum infection levels on the prevalence and levels of bacterial kidney disease (BKD) in progeny fish was conducted at a production salmon hatchery. A total of 302 mating pairs of spring chinook salmon Oncorhynchus tshawytscha was screened in August 1988 for R. salmoninarum by an enzyme-linked immunosorbent assay (ELISA). On the...
Authors
Ronald J. Pascho, Diane G. Elliott, Jonathan M. Streufert

Infectious hematopoietic necrosis (IHN) and viral hemorrhagic septicemia (VHS): Detection of the trout antibodies to the causative viruses by means of plaque neutralization, immunofluorescence, and enzyme-linked immunosorbent assay Infectious hematopoietic necrosis (IHN) and viral hemorrhagic septicemia (VHS): Detection of the trout antibodies to the causative viruses by means of plaque neutralization, immunofluorescence, and enzyme-linked immunosorbent assay

Sera collected from cultured rainbow trout Oncorhynchus mykiss surviving outbreaks of infectious hematopoietic necrosis (IHN) or viral hemorrhagic septicemia (VHS) were examined for the presence of antibodies to both of the causative viruses, infectious hematopoietic necrosis virus (IHNV) and Egtved virus (viral hemorrhagic septicemia virus: VHSV). Sera were screened with three...
Authors
P. E. Vestergard Jorgensen, N.J. Olesen, N. Lorenzen, J. R. Winton, S.S. Ristow

Effects of temperature on early-life-history stages of California halibut Paralichthys californicus Effects of temperature on early-life-history stages of California halibut Paralichthys californicus

- Laboratory experiments were conducted to determine how growth and survival of earlylife-history stages of California halibut Pamlichthys californicus are influenced by temperature, and how optimal temperature ranges may change with ontogeny. As halibut developed from eggs to juveniles, highest survival occurred at increasingly higher temperature ranges. Within tolerance limits, growth...
Authors
D.M. Gadomski, S.M. Caddell
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