Publications

Applications of 10 therapeutic and two anesthetic agents to healthy smolts of coho salmon (Oncorhynchus kisutch) by conventional methods were followed by two different posttreatment circumstances. In condition I, fish were treated and then transferred directly to 28‰ seawater for 10 days; in condition II, fish were treated and held in fresh water for 4 days before their medium was...

Sockeye salmon (Oncorhynchus nerka) were immersion-vaccinated in suspensions containing 5 × 107, 5 × 106, 5 × 105, or 5 × 104 bacteria/mL of bivalent or monovalent, formalin-killedVibrio anguillarum, Types I and II. The fish were split into two lots and held for 54 d. At that time one lot was challenged with living, virulent V. anguillarum, Type I, and one with living, virulent V...

Juvenile coho salmon and steelhead trout ofdifferentstocks and three transferrin genotypes(AA, AC, and CCl, all reared in identical or similar environments, were experimentally infected with Corynebacterium sp., the causative agent ofbacterial kidney disease, or with Vibrio anguillarum, the causative agent of vibriosis. Mortality due to the pathogens was compared among stocks within a...

Ozone and chlorine inactivation curves were determined in three types of freshwater at 20 C for the destruction of the fish pathogens Aeromonas salmonicida the etiologic agent of furunculosis, and Yersinia ruckeri the enteric redmouth bacterium (ERM). Ozone and chlorine inactivation curves were also obtained in the same water types at 10 C for the fish pathogenic viruses infectious...

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The virucidal properties of iodophor, chlorine (sodium hypochlorite), formalin, thimerosal (organic mercurial compound), malachite green, and acriflavine were tested on infectious pancreatic necrosis virus (IPNV). Iodine and chlorine showed good activity, but efficacy depended on the concentration of virus, the presence of organic matter (calf serum), and water pH. Water hardness (0-300...

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An economical, efficacious vaccine delivery system for immunizing fish has been developed which employs a liquid spray apparatus operated at pressures up to 7.0 kg/cm2 (0 to 100 lb/in2). A bacterin consisting of formalin-killed Vibrio anguillarum culture was both antigenic and immunogenic when sprayed on coho salmon(Oncorhynchus kisutch)and rainbow trout(Salmo gairdneri). The technique...

Ozone and chlorine inactivation curves were determined in three water types at 10 °C for the fish pathogenic viruses infectious hematopoietic necrosis (IHNV) and infectious pancreatic necrosis (IPNV). In phosphate-buffered, distilled water (PBDW) an ozone dose of 0.01 mg/L for 30 or 60 s inactivated IHNV or IPNV, respectively, suspended at a tissue culture 50% infective dose (TCID50) of...

Four capture methods were used to collect domesticated rainbow trout (Salmo gairdneri): angling, electroshocking, seining, and direct netting (control). Blood was sampled rapidly upon capture, usually within 2 min. No significant differences were noted within the time frame of the experiment between the four capture groups for plasma protein concentration, lactate dehydrogenase activity...