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Astragalus species complex genetic data from southeast Utah (Grand County and San Juan County), USA

April 11, 2021

These data were compiled to investigate the evolutionary history of Astragalus iselyi, A. sabulosus var. sabulosus, and A. sabulosus var. vehiculus. The data release consists of six text files. One file is a bash script (astragalus_MLE_msms.sh) for generating simulated genetic data. Four files contain individual-level (astragalus.fasta, astragalus.nothin.recode.vcf, astragalus.recode.vcf) or population-level (astragalus_dadi.txt) information on genetic variation. One file contains sampling site-specific data for various soil and climatic variables (astragalus_env.txt). The .vcf files contain the full sequence information that is contained in the other files, but the file structures vary based on the programs used for analysis. These files may be opened and edited in a text editor program, such as Notepad ++ (PC) or BBEdit (Mac). The .vcf file can be loaded into the Stacks population program (Catchen et al. 2013) or VCFtools (Danecek et al. 2011) to calculate genetic diversity statistics (astragalus.nothin.recode.vcf contains multiple genetic variants per locus, whereas astragalus.recode.vcf contains only one genetic variant per locus).The astragalus_dadi.txt file can be used as an input file with the program dadi (Gutenkunst et al. 2009) to infer population history. Finally, astragalus.fasta can be loaded as an input file into RAxML (Stamatakis 2008) to perform phylogenetic analyses.