Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023
December 14, 2023
Reported here are the fluorescence data points recorded from CRISPR/Cas12a biosensor assays to detect the presence or absence of DNA from Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome in bats, from artificial controls, dermal swab, and guano samples. Ct values from qPCR experiments are also provided in a table. Furthermore, we also provide the GenBank accession numbers used to develop the aforementioned CRISPR/Cas12a biosensor assay for Pd DNA detection.
Citation Information
Publication Year | 2023 |
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Title | Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023 |
DOI | 10.5066/P9GYTRUZ |
Authors | Adam A Perez, Abigail Tobin, John V Stechly, Jason A Ferrante, Margaret (Maggie) E. Hunter |
Product Type | Data Release |
Record Source | USGS Asset Identifier Service (AIS) |
USGS Organization | Wetland and Aquatic Research Center - Gainesville, FL |
Rights | This work is marked with CC0 1.0 Universal |
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A minimally invasive, field-applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white-nose syndrome in bats
The accessibility to CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein) genetic tools has given rise to applications beyond site-directed genome editing for the detection of DNA and RNA. These tools include precise diagnostic detection of human disease pathogens, such as SARS-CoV-2 and Zika virus. Despite the technology being rapid and cost-effective,
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A minimally invasive, field-applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white-nose syndrome in bats
The accessibility to CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein) genetic tools has given rise to applications beyond site-directed genome editing for the detection of DNA and RNA. These tools include precise diagnostic detection of human disease pathogens, such as SARS-CoV-2 and Zika virus. Despite the technology being rapid and cost-effective,
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Adam Alberto Perez, Abigail Tobin, John V. Stechly, Jason Ferrante, Margaret Hunter