Margaret Hunter, Ph.D.
Biography
EDUCATION
Ph.D., Veterinary Medical Sciences, University of Florida
B.S., Microbiology and Cell Sciences (Minor: Chemistry, Plant Molecular and Cellular Biology), University of Florida
RESEARCH
Margaret Hunter attended the University of Florida where she received a B.Sc. in Microbiology and Cellular Sciences and minored in Chemistry and Plant Molecular and Cellular Biology. Following her bachelor degree, she received a PhD in Veterinary Medical Sciences researching Sirenia (manatee and dugong) cytogenetics and conservation genetics. Affiliations: USGS Sirenia Project USGS Genetics and Genomics Research Courtesy Assistant Professor, University of Florida, College of Veterinary Medicine, Department of Large Animal Clinical Sciences Affiliated faculty member, University of Florida Aquatic Animal Health Program Affiliated faculty member, University of Florida Genetics Institute.
Science and Products
Health Effects and Behavioral Response of Florida Manatees (Trichechus manatus latirostris) to Persistent Algal Bloom and Associated Loss of Seagrass Resources in Brevard County, Florida
USGS researchers are working with partners to assess the health and foraging behavior of Florida manatees in the northern Indian River Lagoon and Banana River, areas that have experienced declining seagrasses due to an extended phytoplankton bloom.
West Indian Manatee Population Genetics
Nuclear microsatellite markers have been developed and implemented on ~2,000 West Indian manatees. These markers provide individual genetic fingerprints for mark-recapture studies, population structure information for the conservation of unique or isolated populations, and pedigree and relatedness information for addressing inbreeding and breeding structure patterns.
Manatee Health Assessment and Biomedical Studies
A multi-agency effort assesses the health of manatees and provides baseline information on their health, reproductive status, and nutritional condition.
Statistical Models for the Design and Analysis of Environmental DNA (eDNA) Surveys of Invasive and Imperiled Species
Detecting invasive species at low densities or prior to population establishment is critical for successful control and eradication. For example, Burmese pythons occupy thousands of square kilometers of mostly inaccessible habitats.
Trojan Y Invasive Species Control - Sex Marker Identification
To control or possibly eliminate non-native species without harm to native fauna, a genetic technique using sex-reversed females with two Y chromosomes (Trojan Y) is being developed to reduce the breeding success of the species, ultimately resulting in population decline or loss.
Asian Tiger Shrimp Distribution and Genetics
The Asian tiger shrimp began appearing in commercial shrimp catches in 2006. They grow larger than native shrimp and are known to be fierce predators - so shrimpers and managers are concerned about the potential effects this species might have.
Developing Detection Probabilities and Quantifying the Effects of Flowing Water to Improve Asian Carp Environmental DNA (eDNA) Surveys
Invasive Asian carp are problematic for native species, and managers are implementing control measures without well-quantified detection limits or a means to assess the accuracy and precision of existing or future survey data for the fish. Environmental DNA - eDNA - is already used to detect the presence of invasive species, and can be used to identify locations to focus carp control efforts...
Efficacy of eDNA as an Early Detection and Rapid Response Indicator for Burmese Pythons in the Northern Greater Everglades Ecosystem
Traditional approaches to locating Burmese pythons - including visual searches and trapping - have resulted in low detection. Environmental DNA - or eDNA - is increasingly being used to detect the presence of non-native species, particularly when traditional methods may not be adequate.
Genetic Analysis of the Invasive Burmese Python to Aid Management and Population-Control Decision-Making
Invasive Burmese pythons threaten the success of Everglades restoration efforts. To assist with management and population control decision making, USGS scientists are implementing genetic studies to identify potential new entry pathways and to help quantify the size of the breeding population.
Genetic Analysis of Wild and Captive Black Carp in the Mississippi River Basin
Black carp have likely been present in the Mississippi River since the 1990s, but their current distribution and spread is not well understood. Genetics is helping to shed light on this species, including its diversity, the relatedness of wild and captive fish, and its introduction history.
Using Environmental DNA for Burmese Python Detection Probabilities and Range-Delimitation in Southern Florida
Current tools for detection of Burmese pythons in South Florida have resulted in low detection rates. Environmental DNA - eDNA - has shown to be effective at detecting these invasive snakes, and can help to determine range limits for the species, information that is critical for management and control efforts.
Life history, genetics, range expansion and new frontiers of the lionfish (Pterois volitans, Perciformes: Pteroidae) in Latin America
Pterois volitans (lionfish) is a midsize predatory fish commonly found in waters of the western Pacific and Indian Ocean. The species was first documented in Dania Beach, Florida (northwestern Caribbean) in 1985. Since that time the species has expanded its range rapidly to the Northwestern Atlantic Ocean, Gulf of Mexico, and Caribbean Sea....
Díaz-Ferguson, Edgardo; Hunter, Margaret Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem
Environmental DNA (eDNA) detection of invasive species can be used to delimited occupied ranges and estimate probabilities to inform management decisions. Environmental DNA is shed into the environment through skin cells and bodily fluids and can be detected in water samples collected from lakes, rivers, and swamps. In south Florida, invasive...
Hunter, Margaret; Meigs-Friend, Gaia; Ferrante, Jason; Smith, Brian; Hart, Kristen Environmental DNA sampling reveals high occupancy rates of invasive Burmese pythons at wading bird breeding aggregations in the central Everglades
The Burmese python (Python bivittatus) is now established as a breeding population throughout south Florida, USA. However, the extent of the invasion, and the ecological impacts of this novel apex predator on animal communities are incompletely known, in large part because Burmese pythons (hereafter “pythons”) are extremely cryptic and there has...
Orzechowski, Sophia C. M.; Frederick, Peter C.; Dorazio, Robert M.; Hunter, Margaret Cytonuclear discordance in the Florida Everglades invasive Burmese python (Python bivittatus) population reveals possible hybridization with the Indian python (P. molurus)
The invasive Burmese python (Python bivittatus) has been reproducing in the Florida Everglades since the 1980s. These giant constrictor snakes have caused a precipitous decline in small mammal populations in southern Florida following escapes or releases from the commercial pet trade. To better understand the invasion pathway and genetic...
Hunter, Margaret E.; Johnson, Nathan A.; Smith, Brian J.; Davis, Michelle C.; Butterfield, John S.; Snow, Ray W.; Hart, Kristen M. A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
ObjectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA...
Ferrante, Jason A.; Giles, Michelle R.; Benzie, Emily; Hunter, Margaret Next‐generation conservation genetics and biodiversity monitoring
This special issue of Evolutionary Applications consists of 10 publications investigating the use of next‐generation tools and techniques in population genetic analyses and biodiversity assessment. The special issue stems from a 2016 Next Generation Genetic Monitoring Workshop, hosted by the National Institute for Mathematical and...
Hunter, Margaret E.; Hoban, Sean M.; Bruford, Michael W.; Segelbacher, Gernot; Bernatchez, Louis The Florida manatee (Trichechus manatus latirostris) T cell receptor loci exhibit V subgroup synteny and chain-specific evolution
The Florida manatee (Trichechus manatus latirostris) has limited diversity in the immunoglobulin heavy chain. We therefore investigated the antigen receptor loci of the other arm of the adaptive immune system: the T cell receptor. Manatees are the first species from Afrotheria, a basal eutherian superorder, to have an in-depth...
Breaux, Breanna; Hunter, Margaret; Cruz-Schneider, Maria Paula; Sena, Leonardo; Bonde, Robert K.; Criscitiello, Michael F. Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations
Environmental DNA (eDNA) detection is a technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. For eDNA measurement, genetic material shed into the environment is concentrated from water samples and analyzed for the presence of target species. Cytochrome...
Hunter, Margaret; Meigs-Friend, Gaia; Ferrante, Jason A.; Takoukam Kamla, Aristide; Dorazio, Robert; Keith Diagne, Lucy; Luna, Fabia; Lanyon, Janet M.; Reid, James P. Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early...
Ferrante, Jason A.; Hunter, Margaret; Wellehan, James F.X. Genetic composition and connectivity of the Antillean manatee (Trichechus manatus manatus) in Panama
Genetic diversity and haplotype composition of the West Indian manatee (Trichechus manatus) population from the San San Pond Sak wetland in Bocas del Toro, Panama was studied using a segment of mitochondrial DNA (D’loop). No genetic information has been published to date for Panamanian populations. Due to the secretive behavior and small...
Díaz-Ferguson, Edgardo; Hunter, Margaret; Guzmán, Héctor M. The Florida manatee (Trichechus manatus latirostris) immunoglobulin heavy chain suggests the importance of clan III variable segments in repertoire diversity
Manatees are a vulnerable, charismatic sentinel species from the evolutionarily divergent Afrotheria. Manatee health and resistance to infectious disease is of great concern to conservation groups, but little is known about their immune system. To develop manatee-specific tools for monitoring health, we first must have a general knowledge of how...
Breaux, Breanna; Deiss, Thaddeus C.; Chen, Patricia L.; Cruz-Schneider, Maria Paula; Sena, Leonardo; Hunter, Margaret E.; Bonde, Robert K.; Criscitiello, Michael F. Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing...
Hunter, Margaret; Dorazio, Robert M.; Butterfield, John S.; Meigs-Friend, Gaia; Nico, Leo; Ferrante, Jason A.
New genetic test detects manatees' recent presence in fresh or saltwater
Environmental DNA picks up traces of the elusive mammals’ saliva, skin, waste, or exhaled breaths.
Taming the Lion(fish)
Genetics and tracking helps USGS researchers learn where the invasive fish are now – and where they may go next.
Magical Manatees
It may be hard to believe the legend that sailors long-at-sea once believed manatees to be mermaids. The manatee nickname the “Sea Cow” – named so for their affinity for grazing on vegetation and their slow, ambling way – just makes more sense. But a new U.S. Geological Survey video reveals that while they may be cow-like, they also have more than a bit of magical mermaid to them.
Genetics Provides New Clues about Lionfish Invasion
New genetic data suggest the red lionfish invasion in the Caribbean Basin and Western Atlantic started in multiple locations, not just one as previously believed, according to a new study led by the U.S. Geological Survey.