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Jason Ferrante , Ph.D.

Jason is a biologist in the Conservation Genetics Lab at the USGS Wetland and Aquatic Research Center. 

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deer with CWD

Chronic Wasting Disease (CWD) Detection in Cervids Using MicroRNA Biomarkers from Blood

WARC scientists are testing and developing methods for the isolation of cervid exosomes from blood samples, followed by microRNA extraction and next generation sequencing.
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Wetland and Aquatic Research Center
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Chronic Wasting Disease (CWD) Detection in Cervids Using MicroRNA Biomarkers from Blood

WARC scientists are testing and developing methods for the isolation of cervid exosomes from blood samples, followed by microRNA extraction and next generation sequencing.
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USGS science for a changing world logo

Implementing FAIR practices: Storing and displaying eDNA data in the USGS Nonindigenous Aquatic Species database

We are working to incorporate environmental DNA (eDNA) data into the Nonindigenous Aquatic Species (NAS) database, which houses over 570,000 records of nonindigenous species nationally, and already is used by a broad user-base of managers and researchers regularly for invasive species monitoring. eDNA studies have allowed for the identification and biosurveillance of numerous invasive and threaten
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Community for Data Integration (CDI)
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Implementing FAIR practices: Storing and displaying eDNA data in the USGS Nonindigenous Aquatic Species database

We are working to incorporate environmental DNA (eDNA) data into the Nonindigenous Aquatic Species (NAS) database, which houses over 570,000 records of nonindigenous species nationally, and already is used by a broad user-base of managers and researchers regularly for invasive species monitoring. eDNA studies have allowed for the identification and biosurveillance of numerous invasive and threaten
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USGS science for a changing world logo

Establishing standards and integrating environmental DNA (eDNA) data into the USGS Nonindigenous Aquatic Species database

Environmental DNA (eDNA) testing allows for high sensitivity monitoring efforts of cryptic species in large, remote systems and is performed by investigating water and soil samples for sloughed DNA. Having access to eDNA datasets across multiple taxa and ecosystems is necessary for improved coordination among researchers and management. Additionally, quality control protocols are needed to vet inc
By
Community for Data Integration (CDI)
link

Establishing standards and integrating environmental DNA (eDNA) data into the USGS Nonindigenous Aquatic Species database

Environmental DNA (eDNA) testing allows for high sensitivity monitoring efforts of cryptic species in large, remote systems and is performed by investigating water and soil samples for sloughed DNA. Having access to eDNA datasets across multiple taxa and ecosystems is necessary for improved coordination among researchers and management. Additionally, quality control protocols are needed to vet inc
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Genome-wide SNP analysis of three moose subspecies at the southern range limit in the contiguous United States

Genome-wide evaluations of genetic diversity and population structure are important for informing management and conservation of trailing-edge populations. North American moose (Alces alces) are declining along portions of the southern edge of their range due to disease, species interactions, and marginal habitat, all of which may be exacerbated by climate change. We employed a genotyping by seque
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Ecosystems, Wetland and Aquatic Research Center

Lipidomics reveals specific lipid molecules associated with cold stress syndrome in the Florida manatee (Trichechus manatus latirostris)

Cold stress syndrome (CSS) in the Florida manatee (Trichechus manatus latirostris) results in perturbations to many physiological pathways, often leading to further illness or death. In this study, we applied a non-targeted lipidomics approach with ultra-high performance liquid chromatography and high-resolution tandem mass spectrometry to characterize changes related to CSS in the lipidomic profi
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Ecosystems, Wetland and Aquatic Research Center

Environmental DNA (eDNA) detection of nonnative bullseye snakehead in southern Florida

Bullseye Snakehead Channa marulius (Hamilton 1822) was first detected in the southern Florida town of Tamarac in 2000 and has been expanding its geographic range since. Environmental DNA (eDNA) analysis is a newly-developed technique used to noninvasively detect cryptic or low-density species or those that are logistically difficult-to-study. Genetic material shed into the environment through tis
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Ecosystems, Wetland and Aquatic Research Center

Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem

Environmental DNA (eDNA) detection of invasive species can be used to delimited occupied ranges and estimate probabilities to inform management decisions. Environmental DNA is shed into the environment through skin cells and bodily fluids and can be detected in water samples collected from lakes, rivers, and swamps. In south Florida, invasive Burmese pythons occupy much of the Greater Everglades i
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Ecosystems, Wetland and Aquatic Research Center

Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques

To inform management and conservation decisions, environmental DNA (eDNA) methods are used to detect genetic material shed into the water by imperiled and invasive species. Methodological enhancements are needed to reduce filter clogging, PCR inhibition, and false-negative detections when eDNA is at low concentrations. In the first of three simple experiments, we sought to ameliorate filter cloggi
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Ecosystems, Wetland and Aquatic Research Center

A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation

ObjectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA
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Ecosystems, Wetland and Aquatic Research Center

Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations

Environmental DNA (eDNA) detection is a technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. For eDNA measurement, genetic material shed into the environment is concentrated from water samples and analyzed for the presence of target species. Cytochrome bquantitative PCR and droplet digital PCR eDNA assays were develop
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Ecosystems, Wetland and Aquatic Research Center

Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)

Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early immune changes in endangered Florida manatees (Trichechu
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Ecosystems, Wetland and Aquatic Research Center

Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA

A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent or subjec
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Ecosystems, Wetland and Aquatic Research Center

Single Nucleotide Polymorphism (SNP) genomic data of moose (Alces alces) from the contiguous United States, 2009-2017

We analyzed the single nucleotide polymorphisms (SNPs) present in the genomes of moose representing 3 subspecies in the contiguous United States. Blood samples were collected opportunistically from collaborators during field efforts or were supplied to our lab from collaborators' archives, and represented moose sampling occurring between 2009-2017. DNA was extracted, sequenced using next generatio
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Wetland and Aquatic Research Center

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