The files in this data release are RNA seq datafiles from a study that examined the effects of the synthetic anabolic steroid 17B. hydroxyestra 4,9,11 trien-3-one, trenbolone (17B.T - CAS 10161-33-8), a common contaminant of wastes from confined animal feeding operations (CAFOs). Japanese quail (Coturnix japonica) were exposed in the egg and through feed to multiple doses of 17βT and liver transcriptomes were examined to identify genes and pathways directly affected by this androgenic compound. RNA was extracted from liver of adults and embryos and analyzed (1x50 bp) on an Illumina HiSeq 2000. NCBI Biosample accessions and the raw counts that were input into the differential expression analysis are provided in this dataset. The raw sequence data are available at the NCBI Sequence Read Archive under Bioproject numbers PRJNA313918 and PRJNA313931.