Natalie Karouna-Renier, Ph.D.
Biography
Natalie Karouna-Renier is a Research Ecologist at the USGS Patuxent Wildlife Research Center, in Beltsville, MD, where she is the leader of the Molecular Toxicology Laboratory. Natalie's expertise lays in understanding, detecting, and predicting the effects of environmental stressors on birds and other wildlife, primarily at the molecular and biochemical level.
Education/Training:
- PhD in Biology from Rensselaer Polytechnic Institute, Troy, NY
- MS in Toxicology from the University of Maryland
- BS in Biology from Colgate University, Hamilton, NY
Research Interests:
Research in Natalie's lab centers on the identification and application of novel genomic and non-genomic indicators for identifying toxicological responses in wildlife. The effects of endocrine active and genotoxic compounds, and the development of tools to assess and predict differences in species sensitivity are of particular interest and focus. Natalie has also conducted multiple studies investigating higher level effects of contaminants on wildlife, fish, aquatic invertebrates, and humans. http://www.usgs.gov/ecosystems/genetics_genomics/environmental_toxicology_birds.html
Science and Products
Immune System Changes and Susceptibility to Disease in Birds Exposed to Environmental Contaminants
Disease dynamics in wildlife are commonly related to changes or increases in environmental stressors that are placed upon an animal. Environmental pollutants are known to affect the immune system of wildlife, resulting in impaired resistance to infection and potential increases in disease outbreaks.
In collaboration with: Jill Jenkins, Ph.D USGS Wetland and Aquatic Research Center;...
Do Neonicotinoid Pesticide Seed Coatings Pose a Hazard to Seed-eating Birds?
Treating or “dressing” of seeds with pesticides is a commonly used method to enhance crop yield in agriculture. Regrettably, such treated seeds can be ingested by wildlife, and depending on the extent of exposure, can cause adverse effects.
Genomic and Behavioral Effects of the Neonicotinoid Imidacloprid in Birds Exposed Through Pesticide-Coated Seeds
The Challenge: Neonicotinoid pesticides act as agonists of nicotinic acetylcholine receptors (nAChRs) and are designed to be lethal to insects while theoretically posing little to no threat to vertebrates. The perceived safety of neonicotinoids has led to a sharp increase in their use in the United States and globally, since they were first introduced in 1994. The use of the neonicotinoid...
Contaminant Exposure, Food Web Transfer and Potential Health Effects on Chesapeake Bay and Delaware Bay Waterbirds
Chesapeake and Delaware Bays are the largest estuaries on the east coast of the United States. They support extensive fisheries and provide critical habitat for many species of wildlife. These Bays are surrounded by vast agricultural andindustrialized regions, with urbanized areas, all of which are a source of pollution that can affect natural resources. We have long monitored contaminants...
Immunomodulation Science Team
Exposure of wildlife, fish, and humans to environmental contaminants is known to cause changes in immune function, which can affect fitness, reproduction and disease resistance. This process, known as immunomodulation, is a major research topic by public health, veterinary, and other medical professionals outside the U.S. Geological Survey (USGS). However it is outside the mission of those...
DNA Barcoding for Identifying Native Bee Species
The Challenge: Traditionally, bee identification has relied on taxonomic methods centered on descriptions of morphological differences between species. However, for many species, separate keys are required for identifying adult males and females and immature life stages. These keys are commonly unavailable. The lack of distinguishing morphological characters useful for separating closely...
Assessing Toxicogenomics Effects of a Synthetic Androgen on Japanese Quail and the Development of an Avian Vitellogenesis Model
The Challenge: Endocrine active chemicals (EAC) are known to interfere with hormonally regulated physiological processes, thereby affecting signaling in the hypothalamic-pituitary-gonadal-liver (HPGL) axis and commonly resulting in reproductive dysfunction. Computational models that relate hormonal and genomic biomarkers within the HPGL axis to the reproductive cycle and ecologically relevant...
The Role of Japanese Quail in Ecotoxicology
The Challenge: Wild birds are exposed throughout their lives to natural and synthetic chemicals that are present in the environment, many of which interfere with the animal’s physiological and developmental systems. Relative concentrations, routes, frequency, and the environment in which chemical exposure occurs will determine to a large extent the bird’s response. Well-designed avian field...
Identifying the Genetic Basis of Avian Susceptibility to Mercury
The Challenge: Mercury is a highly toxic element found throughout our environment. Although it occurs naturally in some environments, human industrial pollution has greatly increased the amount of mercury and the range of environments in which mercury is found. Recent studies have confirmed clear differences in the sensitivity of various bird species to methylmercury. Because the causes of...
Effects of Short Chain Chlorinated Paraffins (SCCPs) on developing birds
The Challenge: Short-Chain Chlorinated Paraffins (SCCPs) are complex technical mixtures of polychlorinated n-alkanes used in lubricants and coolants in metalworking, as flame retardants, and in paints, adhesives, sealants, textiles and polymeric materials, plastics and rubber. SCCPs are of concern because they are globally transported, bioaccumulate in wildlife and humans, and are...
Assessing Adverse Outcomes Associated with Exposure of Birds to Flame Retardants
The Challenge: The use of flame retardants (FRs) as additives in a variety of consumer use products, including plastics, textiles, and electronics, is projected to continue and increase for the foreseeable future. Because of unanticipated environmental problems, some FRs have either been banned, restricted, or are being phased-out and replaced by other new and presumably safer FRs. Regrettably...
Toxicity of Polybrominated Diphenyl Ethers and Other Flame Retardants to Wildlife
Flame retardants are a class of commonly used household and industrial products. Because of widespread use, there is concern that their presence in the environment can potentially pose a hazard and risk to wildlife.
In ovo exposure to brominated flame retardants Part II: Assessment of effects of TBBPA-BDBPE and BTBPE on hatching success, morphometric and physiological endpoints in American kestrels
Tetrabromobisphenol A bis(2,3-dibromopropyl ether) (TBBPA-BDBPE) and 1,2-bis(2,4,6-tribromophenoxy)ethane (BTPBE) are both brominated flame retardants (BFRs) that have been detected in birds; however, their potential biological effects are largely unknown. We assessed the effects of embryonic exposure to TBBPA-BDBPE and BTBPE in a model avian...
Eng, Margaret; Karouna-Renier, Natalie K.; Henry, Paula F. P.; Letcher, Robert J.; Schultz, Sandra L.; Bean, Thomas G.; Peters, Lisa E.; Palace, Vince P.; Williams, Tony D.; Elliott, John E.; Fernie, Kim J. In ovo exposure to brominated flame retardants Part I: Assessment of effects of TBBPA-BDBPE on survival, morphometric and physiological endpoints in zebra finches
Tetrabromobisphenol A bis(2,3-dibromopropyl) ether (TBBPA-BDBPE) is an additive flame retardant used in polyolefins and polymers. It has been detected in biota, including in avian eggs, yet little is known of its effects. We assessed the pattern of TBBPA-BDBPE concentrations in songbird eggs over the incubation period, and the effects of embryonic...
Eng, Margaret; Williams, Tony D.; Fernie, Kim J.; Karouna-Renier, Natalie K.; Henry, Paula F. P.; Letcher, Robert J.; Elliott, John E. Perfluoroalkyl contaminant exposure in tree swallows nesting at Clarks Marsh, Oscoda, Michigan, USA
A site in north eastern Michigan, Oscoda Township, has some of the highest recorded exposure in birds to perfluorinated substances (PFASs) in the U.S. Some egg and plasma concentrations at that location exceeded the lowest reproductive effect threshold established for two avian laboratory species. The objectives of this study were to determine...
Custer, Christine M.; Custer, Thomas W.; Delaney, Robert; Dummer, Paul; Schultz, Sandra L.; Karouna-Renier, Natalie K. Toxicokinetics of imidacloprid-coated wheat seeds in Japanese quail (Coturnix japonica) and an evaluation of hazard
Birds are potentially exposed to neonicotinoid insecticides by ingestion of coated seeds during crop planting. Adult male Japanese quail were orally dosed with wheat seeds coated with an imidacloprid (IMI) formulation at either 0.9 mg/kg body weight (BW) or 2.7 mg/kg BW (~3 and 9% of IMI LD50 for Japanese quail, respectively) for 1 or 10 days....
Bean, Thomas G.; Gross, Michael S.; Karouna-Renier, Natalie K.; Henry, Paula F. P.; Schultz, Sandra L.; Hladik, Michelle; Kuivila, Kathryn; Rattner, Barnett A. Sex‐specific responses in neuroanatomy of hatchling American kestrels in response to embryonic exposure to the flame retardants bis(2‐ethylhexyl)‐2,3,4,5‐tetrabromophthalate and 2‐ethylhexyl‐2,3,4,5‐tetrabromobenzoate
Bis(2‐ethylhexyl)‐2,3,4,5‐tetrabromophthalate (BEH‐TEBP) and 2‐ethylhexyl‐2,3,4,5‐tetrabromobenzoate (EH‐TBB), flame retardant components of FireMaster 550® and 600® have been detected in tissues of wild birds. To address the paucity of information regarding potential impacts of flame retardants on the brain, brain volume regions of hatchling...
Guigueno, Mélanie F.; Karouna-Renier, Natalie K.; Henry, Paula F. P.; Peters, Lisa E.; Palace, Vince P.; Letcher, Robert J.; Fernie, Kim J. Female hatchling American kestrels have a larger hippocampus than males: A link with sexual size dimorphism?
The brain and underlying cognition may vary adaptively according to an organism’s ecology. As with all raptor species, adult American kestrels (Falco sparverius) are sexually dimorphic with females being larger than males. Related to this sexual dimorphism, kestrels display sex differences in hunting and migration, with females ranging more widely...
Guigueno, Melanie F.; Karouna-Renier, Natalie K.; Henry, Paula F. P.; Head, Jessica A.; Peters, Lisa E.; Palace, Vince P.; Letcher, Robert J.; Fernie, Kimberly J. Tributyltin: Advancing the science on assessing endocrine disruption with an unconventional endocrine-disrupting compound
Tributyltin (TBT) has been recognized as an endocrine disrupting chemical (EDC) for several decades. However, only in the last decade, was its primary endocrine mechanism of action (MeOA) elucidated—interactions with the nuclear retinoid-X receptor (RXR), peroxisome proliferator-activated receptor γ (PPARγ), and their heterodimers. This molecular...
Lagadic, Laurent; Katsiadaki, Ioanna; Biever, Ronald C.; Guiney, Patrick; Karouna-Renier, Natalie K.; Schwarz, Tamar; Meador, James P. Biomarker responses of Peromyscus leucopus exposed to lead and cadmium in the Southeast Missouri Lead Mining District
Biomarker responses and histopathological lesions have been documented in laboratory mammals exposed to elevated concentrations of lead and cadmium. The exposure of white-footed mice (Peromyscus leucopus) to these metals and the potential associated toxic effects were examined at three contaminated sites in the Southeast Missouri Lead Mining...
Beyer, W. Nelson; Casteel, Stan W.; Friedrichs, Kristen R.; Gramlich, Eric; Houseright, Ruth A.; Nichols, John W.; Karouna-Renier, Natalie K.; Kim, Dae Young; Rangen, Kathleen; Rattner, Barnett A.; Schultz, Sandra L. Effects on circulating steroid hormones and gene expression along the hypothalamus–pituitary–gonadal axis in adult Japanese quail exposed to 17β-trenbolone across multiple generations
We investigated the effects of the androgenic growth promoter 17β-trenbolone (17βTB) on adult Japanese quail (Coturnix japonica) exposed across three generations. The F0 generation was exposed after sexual maturity to 0, 1, 5, 10, 20, and 40 ppm through feed. The F1 generation was exposed in ovo by maternal transfer and through feed at the same...
Karouna-Renier, Natalie K.; Chen, Yu; Henry, Paula F. P.; Maddox, Catherine M.; Sprague, Dan Current limitations and recommendations to improve testing for the environmental assessment of endocrine active substances
In the present study, existing regulatory frameworks and test systems for assessing potential endocrine active chemicals are described, and associated challenges are discussed, along with proposed approaches to address these challenges. Regulatory frameworks vary somewhat across geographies, but all basically evaluate whether a chemical possesses...
Coady, Katherine K.; Biever, Ronald C.; Denslow, Nancy D.; Gross, Melanie; Guiney, Patrick D.; Holbech, Henrik; Karouna-Renier, Natalie K.; Katsiadaki, Ioanna; Krueger, Hank; Levine, Steven L.; Maack, Gerd; Williams, Mike; Wolf, Jeffrey C.; Ankley, Gerald T. Recommended approaches to the scientific evaluation of ecotoxicological hazards and risks of endocrine-active substances
A SETAC Pellston Workshop® “Environmental Hazard and Risk Assessment Approaches for Endocrine-Active Substances (EHRA)” was held in February 2016 in Pensacola, Florida, USA. The primary objective of the workshop was to provide advice, based on current scientific understanding, to regulators and policy makers; the aim being to make considered...
Matthiessen, Peter; Ankley, Gerald T.; Biever, Ronald C.; Bjerregaard, Poul; Borgert, Christopher; Brugger, Kristin; Blankinship, Amy; Chambers, Janice; Coady, Katherine K.; Constantine, Lisa; Dang, Zhichao; Denslow, Nancy D.; Dreier, David; Dungey, Steve; Gray, L. Earl; Gross, Melanie; Guiney, Patrick D.; Hecker, Markus; Holbech, Henrik; Iguchi, Taisen; Kadlec, Sarah; Karouna-Renier, Natalie K.; Katsiadaki, Ioanna; Kawashima, Yukio; Kloas, Werner; Krueger, Henry; Kumar, Anu; Lagadic, Laurent; Leopold, Annegaaike; Levine, Steven L.; Maack, Gerd; Marty, Sue; Meador, James P.; Mihaich, Ellen; Odum, Jenny; Ortego, Lisa; Parrott, Joanne L.; Pickford, Daniel; Roberts, Mike; Schaefers, Christoph; Schwarz, Tamar; Solomon, Keith; Verslycke, Tim; Weltje, Lennart; Wheeler, James R.; Williams, Mike; Wolf, Jeffery C.; Yamazaki, Kunihiko Chesapeake Bay fish–osprey (Pandion haliaetus) food chain: Evaluation of contaminant exposure and genetic damage
From 2011 to 2013, a large-scale ecotoxicological study was conducted in several Chesapeake Bay (USA) tributaries (Susquehanna River and flats, the Back, Baltimore Harbor/Patapsco Rivers, Anacostia/ middle Potomac, Elizabeth and James Rivers) and Poplar Island as a mid-Bay reference site. Osprey (Pandion haliaetus) diet and the transfer of...
Lazarus, Rebecca S.; Rattner, Barnett A.; McGowan, Peter C.; Hale, Robert C.; Karouna-Reiner, Natalie K.; Erickson, Richard A.; Ottinger, Mary Ann