Invertebrate, fish, and habitat data were collected as part of a cooperative project that began in 2002 between the U.S. Geological Survey, Colorado Springs Utilities, and Colorado Springs Engineering. Other entities have contributed to the project over the years including Colorado Parks and Wildlife. Data were primarily collected from up to 35 sites - 33 sites within the Fountain Creek Basin but also from two sites on the Arkansas River. The number of sites sampled each year as well as the number of annual visits during which data were collected, and sampling methods used during each visit vary. This data release includes data collected between 2002 and 2020 and is intended to be updated annually as new data are collected and available. Users of these data are advised to read all metadata and associated documentation thoroughly to understand appropriate use and data limitations.