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This dataset describes the quantity, morphology, and polymer identity of microplastics in the water column and surficial sediments of Milwaukee-Area streams, the Milwaukee Harbor, and Lake Michigan (Wisconsin). Water samples were collected at 10 locations, 2-4 times each, from May to September, 2016. At the 4 shallowest locations, water was collected only at the water surface. At the remaining 6 locations, water was collected at the water surface and at 1-4 depths below the surface. Sediment samples were collected once, in June 2016, at a subset of 9 locations. Sampling and analysis methods are described in the Processing Steps section of the metadata. These data are interpreted in a journal article (TBD).