Data supporting the article, "Nutrient limitation of benthic algae in streams: integrating laboratory bioassays, field experiments, and field data." Datasets include from a battery of laboratory and in situ nutrient limitation tests in streams in the upper Snake River basin, Idaho. Nutrient limitation bioassays of stream water used the green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) and a model macrophyte (duckweed, Lemna minor) with attached epiphytes; and in situ stream nutrient limitation tests with nutrient diffusing substrates.