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The USGS National Wildlife Health Center's Diagnostic Virology Laboratory (DVL) performs isolation and identification of common and novel viruses from diagnostic and research samples.
Isolation procedures used are specific to the host animal and suspected pathogen. The DVL has expertise in recognizing morphological changes in cell culture and effects on embryonated avian eggs caused by viral infection. Some of the identification techniques used include PCR, RT-PCR, serum neutralization, serology, electron microscopy, and DNA sequencing. The DVL participates in the Select Agent Program and is a member of the USDA National Animal Health Laboratory Network (NAHLN).
Virus isolation and propagation:
Identification of virus isolates:
Characterization of novel virus isolates:
Serologic tests (antibody detection):
Nucleic acid extraction, screening, and sequencing:
The following is a reference guide to commonly requested tests. It is not a full list of testing capabilities of the laboratories. Determination of which test will be run on any given submission will be based on case history, gross findings at necropsy, and the scientific judgment of the case pathologist.