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Draft genome sequence of Bordetella sp. strain FB-8, isolated from a former uranium mining area in Germany Draft genome sequence of Bordetella sp. strain FB-8, isolated from a former uranium mining area in Germany

Here, we present the draft genome sequence of Bordetella sp. strain FB-8, a mixotrophic iron-oxidizing bacterium isolated from creek sediment in the former uranium-mining district of Ronneburg, Germany. To date, iron oxidation has not been reported in Bordetella species, indicating that FB-8 may be an environmentally important Bordetella sp.
Authors
Cassandra Rashan Harris, Denise M. Akob, Maria Fabisch, Felix Beulig, Tanya Woyke, Nicole Shapiro, Alla Lapidus, Hans-Peter Klenk, Kirsten Küsel

Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture

In aquifers, acetylene (C2H2) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals. C2H2 can, in turn, inhibit multiple microbial processes including TCE dechlorination and metabolisms that commonly support dechlorination, in addition to supporting the growth of acetylenotrophic microorganisms. Previously, C2H2 was shown to support TCE reductive...
Authors
Sara Gushgari-Doyle, Ronald S. Oremland, Ray Keren, Shaun Baesman, Denise M. Akob, Jillian F. Banfield, Lisa Alvarez-Cohen

Mixotrophic iron-oxidizing Thiomonas isolates from an acid mine drainage-affected creek Mixotrophic iron-oxidizing Thiomonas isolates from an acid mine drainage-affected creek

Natural attenuation of heavy metals occurs via coupled microbial iron cycling and metal precipitation in creeks impacted by acid mine drainage (AMD). Here, we describe the isolation, characterization, and genomic sequencing of two iron-oxidizing bacteria (FeOB) species: Thiomonas ferrovorans FB-6 and Thiomonas metallidurans FB-Cd, isolated from slightly acidic (pH 6.3), Fe-rich, AMD...
Authors
Denise M. Akob, Michelle Hallenbeck, Felix Beulig, Maria Fabisch, Kirsten Kusel, Jessica L Keffer, Tanja Woyke, Nicole Shapiro, Alla Lapidus, Hans-Peter Klenk, Clara Chan

Geochemical and geophysical indicators of oil and gas wastewater can trace potential exposure pathways following releases to surface waters Geochemical and geophysical indicators of oil and gas wastewater can trace potential exposure pathways following releases to surface waters

Releases of oil and gas (OG) wastewaters can have complex effects on stream-water quality and downstream organisms, due to sediment-water interactions and groundwater/surface water exchange. Previously, elevated concentrations of sodium (Na), chloride (Cl), barium (Ba), strontium (Sr), and lithium (Li), and trace hydrocarbons were determined to be key markers of OG wastewater releases...
Authors
Isabelle M. Cozzarelli, Douglas B. Kent, Martin A. Briggs, Mark A Engle, Adam Benthem, Katherine Skalak, Adam Mumford, Jeanne B. Jaeschke, Aida Farag, John W. Lane, Denise M. Akob

Compositional analysis of formation water geochemistry and microbiology of commercial and carbon dioxide-rich wells in the southwestern United States Compositional analysis of formation water geochemistry and microbiology of commercial and carbon dioxide-rich wells in the southwestern United States

Studies of naturally occurring subsurface carbon dioxide (CO2) accumulations can provide useful information for potential CO2 injection projects; however, the microbial communities and formation water geochemistry of most reservoirs are understudied. Formation water and microbial biomass were sampled at four CO2-rich reservoir sites: two within Bravo Dome, a commercial CO2 field in New...
Authors
Jenna L. Shelton, Robert S. Andrews, Denise M. Akob, Christina A. DeVera, Adam C. Mumford, Mark Engle, Michelle R. Plampin, Sean T. Brennan

Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania

The number of horizontally drilled shale oil and gas wells in the United States has increased from nearly 28,000 in 2007 to nearly 127,000 in 2017, and research has suggested the potential for the development of shale resources to affect nearby stream ecosystems. However, the ability to generalize current studies is limited by the small geographic scope as well as limited breadth and...
Authors
Adam Mumford, Kelly O. Maloney, Denise M. Akob, Sarah Nettemann, Arianne Proctor, Jason Ditty, Luke Ulsamer, Josh Lookenbill, Isabelle M. Cozzarelli

Binning singletons: Mentoring through networking at ASM microbe 2019 Binning singletons: Mentoring through networking at ASM microbe 2019

The American Society for Microbiology (ASM) national conference, Microbe, is the flagship meeting for microbiologists across the globe. The presence of roughly 10,000 attendees provides enormous opportunities for networking and learning. However, such a large meeting can be intimidating to many, especially early career scientists, students, those attending alone, and those from...
Authors
Joseph B. James, Amanda L. Gunn, Denise M. Akob

Draft genome sequence of the Mn(II)-oxidizing bacterium Oxalobacteraceae sp. AB_14 Draft genome sequence of the Mn(II)-oxidizing bacterium Oxalobacteraceae sp. AB_14

Biological Mn(II) oxidation produces reactive manganese oxides that help to mitigate metal contamination in the environment. Here we present the genome of Oxalobacteraceae sp. AB_14, a species of Mn(II)-oxidizing bacteria (MOB) notable for its ability to catalyze Mn oxidation at low pH (5.5).
Authors
Timothy Bushman, Denise M. Akob, Tsing Bohu, Andrea Beyer, Tanja Woyke, Nicole Shapiro, Alla Lapidus, Hans-Peter Klenk, Kirsten Küsel

Phylogenetic techniques in geomicrobiology Phylogenetic techniques in geomicrobiology

Molecular biological techniques have revolutionized the field of geomicrobiology by providing researchers with robust techniques for identifying microorganisms and characterizing microbial communities in a wide variety of environments. These techniques have freed researchers from the constraints of classical culture-based microbiology and allowed the discovery of previously unknown...
Authors
Denise M. Akob, Adam C. Mumford, Darren S. Dunlop, Amisha T. Poret-Peterson

Organic geochemistry and toxicology of a stream impacted by unconventional oil and gas wastewater disposal operations Organic geochemistry and toxicology of a stream impacted by unconventional oil and gas wastewater disposal operations

Water and sediment extracts samples were analyzed for extractable hydrocarbons by gas chromatography/mass spectrometry (GC/MS) using an Agilent (Agilent Technologies, Palo Alto, CA, USA) 7890 series GC and 5975 electron ionization (EI) mass selective detector (MSD) operated in scan mode. Agilent ChemStation software was used for data acquisition and analysis (version E.02.00.493 on GC/MS...
Authors
William H. Orem, Matthew S. Varonka, Lynn M. Crosby, Karl B. Haase, Keith A. Loftin, Michelle L. Hladik, Denise M. Akob, Calin Tatu, Adam C. Mumford, Jeanne B. Jaeschke, Anne L. Bates, Tiffani Schell, Isabelle M. Cozzarelli

Microbial community composition of a hydrocarbon reservoir 40 years after a CO2 enhanced oil recovery flood Microbial community composition of a hydrocarbon reservoir 40 years after a CO2 enhanced oil recovery flood

Injecting CO2 into depleted oil reservoirs to extract additional crude oil is a common enhanced oil recovery (CO2-EOR) technique. However, little is known about how in situ microbial communities may be impacted by CO2 flooding, or if any permanent microbiological changes occur after flooding has ceased. Formation water was collected from an oil field that was flooded for CO2-EOR in the...
Authors
Jenna L. Shelton, Robert S. Andrews, Denise M. Akob, Christina A. DeVera, Adam C. Mumford, John E. McCray, Jennifer C. McIntosh

An initial comparison of pesticides and amphibian pathogens between natural and created wetlands in the New Jersey Pinelands, 2014–16 An initial comparison of pesticides and amphibian pathogens between natural and created wetlands in the New Jersey Pinelands, 2014–16

A study conducted by the U.S. Geological Survey, in cooperation with the New Jersey Pinelands Commission and Montclair State University, was designed to compare pesticide concentrations and the presence and prevalence of amphibian pathogens between natural ponds and two types of created wetlands, excavated ponds and stormwater basins, throughout the New Jersey Pinelands. The study...
Authors
Kelly L. Smalling, John F. Bunnell, Jonathan Cohl, Kristin M. Romanok, Lisa Hazard, Kirsten Monsen, Denise M. Akob, Angela M. Hansen, Michelle L. Hladik, Nicole Abdallah, Quratulain Ahmed, Araba Assan, Matthew D. De Parsia, Amaryl Griggs, Megan McWayne-Holmes, Naisargi Patel, Corey Sanders, Yesha Shrestha, Sean M. Stout, Brianna Williams
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