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In the charred groves of California’s Sierra Nevada, silence has replaced the towering presence of some of the world’s largest trees. Once standing for thousands of years, these ancient giant sequoias have withstood centuries of fire—until now.

In just the past few years, wildfires, exacerbated by heavy fuel loads, warming temperatures and exceptional drought have killed nearly 20% of the world’s mature sequoias. Fires like the Castle Fire in 2020 and the KNP Complex Fire in 2021 burned hotter and more intensely than anything these forests had evolved to survive. And in some areas, something even more unsettling is happening: the groves are not growing back.

While these massive trees once relied on fire to open their cones and create space for seedlings, today’s megafires can potentially consume entire cone crops and leave behind soil that is too dry, too hot, and possibly too depleted of life to support new growth. Now, a team of scientists from the U.S. Geological Survey (USGS), the National Park Service, Stanford University, the University of Washington, in collaboration with other partners, is trying to figure out how to bring these forests back—starting from the ground up.

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Plant seedlings growing on forest floor
Naturally regenerating giant sequoia seedlings are growing in an area of Giant Forest, a sequoia grove in Sequoia National Park, that burned at low intensity during the 2021 KNP Complex Fire.
Burned sequoia grove

The Secret Life Beneath the Forest Floor

At the heart of the project is a tiny but powerful community: soil microbes. These fungi and bacteria live on and around tree roots, forming partnerships that help plants absorb water and nutrients, fight off disease, and tolerate drought.

“Without the right microbes, seedlings may struggle to establish,” says Dr. Courtney Creamer, a USGS scientist leading the research. “High-intensity fires may reduce the diversity or abundance of beneficial soil microbes, especially those that support plant growth. We want to know: can reintroducing those microbes help seedlings survive in tough, post-fire landscapes?”

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Scientist holding giant sequoia seedling root (left) and sequoia seedling in scientific instrument (right).
Ellie Fajer holding a giant sequoia seedling's root mass (left) that has been carefully excavated to collect root tips for examining arbuscular mycorrhizal associations. At right, a seedling is placed in a pressure chamber designed to measure plant water potential.

To find out, the team is growing hundreds of sequoia seedlings in different soil mixes—some from unburned forests, some from severely burned sites. Some soils are treated with beneficial fungi like arbuscular mycorrhizal fungi (AMF), known to support root development and water uptake. The goal is to test whether microbial “boosts” can give seedlings a better shot at surviving after being planted back in the wild.

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Scientist plants seedlings in cone-tainers (left) and nursery manager waters seedlings (right).
Courtney Creamer (left), USGS project lead, prepares control seedlings by planting in sterile peat cone-tainers—with or without microbial or mycorrhizal inoculants. Jonathan Humphrey (right), nursery manager at the National Park Service’s Ash Mountain Headquarters in Sequoia and Kings Canyon National Parks, waters freshly planted cone-tainers as part of the experimental setup.

Mapping the Landscape, One Slope at a Time

But microbes are only part of the story. Where seedlings are planted may matter just as much as how they’re grown.

The researchers are using high-resolution terrain maps to classify the landscape into “moisture zones” based on slope, sun exposure, and proximity to water. Some areas hold moisture better—key for young trees facing California’s dry summers. By planting seedlings across these different zones and monitoring their success, the team hopes to learn which parts of the burned forest are most likely to support planted seedling growth. Placing each seedling where the soil, microbes, and moisture conditions are most favorable may further improve survival after catastrophic fire.

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Schematic of clustering model and resulting map (left) and USGS scientist standing in front of a sequoia tree.
USGS scientist Lauren Holzman (pictured right) developed a clustering model (shown left) using these layers to group areas into “wet” and “dry” zones—information that helps guide where restoration plantings are most likely to succeed.

Replanting the Future

This isn’t just about saving trees—it’s about protecting ecosystems, cultural heritage, and climate resilience. Giant sequoias play a vital role in their environment, providing habitat, storing carbon, and inspiring awe in millions of visitors each year. 

That’s why the project is deeply collaborative. The team is working with land managers, land stewards, and researchers in academic, government, and non-governmental organizations to ensure the work reflects shared values and benefits.

The stakes are high. If natural regeneration fails, and replanting doesn’t succeed, some sequoia groves could disappear altogether. But if this research can pinpoint where and how to restore them, it could guide reforestation efforts across the Sierra—and possibly inform efforts in other fire-impacted ecosystems.  In a hotter, drier future, targeted restoration strategies like this may be increasingly important.

From microbes to mountainsides, the team is working to ensure that the next generation of sequoias takes root—not just for today, but for centuries to come.

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Group of scientists pose for photo in the field
The 2024 Redwood Mountain field team (right to left): Mark Waldrop, Ellie Fajer, Jack McFarland, Courtney Creamer, Lauren Holzman, Claire Willing, Aubrey Franks, Christy Brigham, and Arie Oosterom.
Sequoia grove at sunrise
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