In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Citation Information
| Publication Year | 2020 |
|---|---|
| Title | Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques |
| DOI | 10.1007/s00705-020-04737-w |
| Authors | Mariano Carossino, Hon S. Ip, Jürgen a. Richt, Kendra Schultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Archives of Virology |
| Index ID | 70216993 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | National Wildlife Health Center |