Donald Tillitt, PhD (Former Employee)
Science and Products
Filter Total Items: 215
Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances
There is a need to adapt cell bioassays to 384-well and 1536-well formats instead of the traditional 96-well format as high-throughput screening (HTS) demands increase. However, the sensitivity and performance of the bioassay must be re-verified in these higher micro-well plates, and verification of cell health must also be HT (high-throughput). We have adapted two commonly used human...
Authors
Jennifer Brennan, Donald E. Tillitt
Grand challenges for integrated USGS science — A workshop report Grand challenges for integrated USGS science — A workshop report
Executive Summary The U.S. Geological Survey (USGS) has a long history of advancing the traditional Earth science disciplines and identifying opportunities to integrate USGS science across disciplines to address complex societal problems. The USGS science strategy for 2007–2017 laid out key challenges in disciplinary and interdisciplinary arenas, culminating in a call for increased focus...
Authors
Karen E. Jenni, Martin B. Goldhaber, Julio L. Betancourt, Jill S. Baron, Sky Bristol, Mary Cantrill, Paul E. Exter, Michael J. Focazio, John W. Haines, Lauren E. Hay, Leslie Hsu, Victor F. Labson, Kevin D. Lafferty, K. A. Ludwig, Paul C. D. Milly, Toni L. Morelli, Suzette A. Morman, Nedal T. Nassar, Timothy R. Newman, Andrea C. Ostroff, Jordan S. Read, Sasha C. Reed, Carl D. Shapiro, Richard A. Smith, Ward E. Sanford, Terry L. Sohl, Edward G. Stets, Adam J. Terando, Donald E. Tillitt, Michael A. Tischler, Patricia L. Toccalino, David J. Wald, Mark P. Waldrop, Anne Wein, Jake F. Weltzin, Christian E. Zimmerman
Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl
The aquatic food web of the Great Lakes has been contaminated with polychlorinated biphenyls (PCBs) since the mid-20th century. Threats of PCB exposures to long-lived species of fish, such as lake sturgeon (Acipenser fulvescens), have been uncertain because of a lack of information on the relative sensitivity of the species. The objective of the present study was to evaluate the...
Authors
Donald E. Tillitt, Justin A. Buckler, Diane Nicks, James Candrl, Rachel Claunch, Robert W. Gale, Holly J. Puglis, Edward E. Little, Tiffany L. Linbo, Mary Baker
Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)
Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression...
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
Adverse reproductive and developmental health outcomes following prenatal exposure to a 2 hydraulic fracturing chemical mixture in female C57Bl/6 mice Adverse reproductive and developmental health outcomes following prenatal exposure to a 2 hydraulic fracturing chemical mixture in female C57Bl/6 mice
Unconventional oil and gas operations using hydraulic fracturing can contaminate surface and groundwater with endocrine-disrupting chemicals. We have previously shown that 23 of 24 commonly used hydraulic fracturing chemicals can activate or inhibit the estrogen, androgen, glucocorticoid, progesterone, and/or thyroid receptors in a human endometrial cancer cell reporter gene assay and...
Authors
Christopher D. Kassotis, John J. Bromfield, Kara C. Klemp, Chun-Xia Meng, Andrew R. Wolfe, Thomas Zoeller, Victoria D. Balise, Chiamaka J. Isiguzo, Donald E. Tillitt, Susan C. Nagel
Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice
Oil and natural gas operations have been shown to contaminate surface and ground water with endocrine-disrupting chemicals. In the current study, we fill several gaps in our understanding of the potential environmental impacts related to this process. We measured the endocrine-disrupting activities of 24 chemicals used and/or produced by oil and gas operations for five nuclear receptors...
Authors
Christopher D. Kassotis, Kara C. Klemp, Danh C. Vu, Chung-Ho Lin, Chun-Xia Meng, Cynthia L. Besch-Williford, Lisa Pinatti, R. Thomas Zoeller, Erma Z. Drobnis, Victoria D. Balise, Chiamaka J. Isiguzo, Michelle A. Williams, Donald E. Tillitt, Susan C. Nagel
Science and Products
Filter Total Items: 215
Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances
There is a need to adapt cell bioassays to 384-well and 1536-well formats instead of the traditional 96-well format as high-throughput screening (HTS) demands increase. However, the sensitivity and performance of the bioassay must be re-verified in these higher micro-well plates, and verification of cell health must also be HT (high-throughput). We have adapted two commonly used human...
Authors
Jennifer Brennan, Donald E. Tillitt
Grand challenges for integrated USGS science — A workshop report Grand challenges for integrated USGS science — A workshop report
Executive Summary The U.S. Geological Survey (USGS) has a long history of advancing the traditional Earth science disciplines and identifying opportunities to integrate USGS science across disciplines to address complex societal problems. The USGS science strategy for 2007–2017 laid out key challenges in disciplinary and interdisciplinary arenas, culminating in a call for increased focus...
Authors
Karen E. Jenni, Martin B. Goldhaber, Julio L. Betancourt, Jill S. Baron, Sky Bristol, Mary Cantrill, Paul E. Exter, Michael J. Focazio, John W. Haines, Lauren E. Hay, Leslie Hsu, Victor F. Labson, Kevin D. Lafferty, K. A. Ludwig, Paul C. D. Milly, Toni L. Morelli, Suzette A. Morman, Nedal T. Nassar, Timothy R. Newman, Andrea C. Ostroff, Jordan S. Read, Sasha C. Reed, Carl D. Shapiro, Richard A. Smith, Ward E. Sanford, Terry L. Sohl, Edward G. Stets, Adam J. Terando, Donald E. Tillitt, Michael A. Tischler, Patricia L. Toccalino, David J. Wald, Mark P. Waldrop, Anne Wein, Jake F. Weltzin, Christian E. Zimmerman
Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl
The aquatic food web of the Great Lakes has been contaminated with polychlorinated biphenyls (PCBs) since the mid-20th century. Threats of PCB exposures to long-lived species of fish, such as lake sturgeon (Acipenser fulvescens), have been uncertain because of a lack of information on the relative sensitivity of the species. The objective of the present study was to evaluate the...
Authors
Donald E. Tillitt, Justin A. Buckler, Diane Nicks, James Candrl, Rachel Claunch, Robert W. Gale, Holly J. Puglis, Edward E. Little, Tiffany L. Linbo, Mary Baker
Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)
Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression...
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
Adverse reproductive and developmental health outcomes following prenatal exposure to a 2 hydraulic fracturing chemical mixture in female C57Bl/6 mice Adverse reproductive and developmental health outcomes following prenatal exposure to a 2 hydraulic fracturing chemical mixture in female C57Bl/6 mice
Unconventional oil and gas operations using hydraulic fracturing can contaminate surface and groundwater with endocrine-disrupting chemicals. We have previously shown that 23 of 24 commonly used hydraulic fracturing chemicals can activate or inhibit the estrogen, androgen, glucocorticoid, progesterone, and/or thyroid receptors in a human endometrial cancer cell reporter gene assay and...
Authors
Christopher D. Kassotis, John J. Bromfield, Kara C. Klemp, Chun-Xia Meng, Andrew R. Wolfe, Thomas Zoeller, Victoria D. Balise, Chiamaka J. Isiguzo, Donald E. Tillitt, Susan C. Nagel
Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice
Oil and natural gas operations have been shown to contaminate surface and ground water with endocrine-disrupting chemicals. In the current study, we fill several gaps in our understanding of the potential environmental impacts related to this process. We measured the endocrine-disrupting activities of 24 chemicals used and/or produced by oil and gas operations for five nuclear receptors...
Authors
Christopher D. Kassotis, Kara C. Klemp, Danh C. Vu, Chung-Ho Lin, Chun-Xia Meng, Cynthia L. Besch-Williford, Lisa Pinatti, R. Thomas Zoeller, Erma Z. Drobnis, Victoria D. Balise, Chiamaka J. Isiguzo, Michelle A. Williams, Donald E. Tillitt, Susan C. Nagel
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government