Kathryn Griffin
Kathryn Griffin is a Biological Science Laboratory Technician at the National Wildlife Health Center.
Science and Products
Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America”
In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, raccoons, and skunks, 261 (72%) of the animals harbored a
Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction Reagent. RHDV2
Authors
Erik K. Hofmeister, Kathryn M. Griffin, Hon S. Ip
An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America
In summer 2020, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from on or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, racc
Authors
Hon S. Ip, Kathryn M. Griffin, Jeffrey D. Messer, Megan Winzeler, Susan A. Shriner, Mary Lea Killian, Mia K. Torchetti, Thomas J. DeLiberto, Brian R. Amman, Caitlin M. Cossaboom, R. Reid Harvey, Natalie M. Wendling, Hannah Rettler, Dean Taylor, Jonathan S. Towner, Casey Barton Behravesh, David S. Blehert
First detection of bat white-nose syndrome in western North America
White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia to naive ho
Authors
Jeffrey M. Lorch, Jonathan M. Palmer, Daniel L. Lindner, Anne Ballmann, Kyle George, Kathryn M. Griffin, Susan Knowles, John R. Huckabee, Katherine H. Haman, Christopher D. Anderson, Penny A. Becker, Joseph B. Buchanan, Jeffrey T. Foster, David S. Blehert
TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease
Background
Fungal skin infections associated with Ophidiomyces ophiodiicola, a member of the Chrysosporiumanamorph of Nannizziopsis vriesii (CANV) complex, have been linked to an increasing number of cases of snake fungal disease (SFD) in captive snakes around the world and in wild snake populations in eastern North America. The emergence of SFD in both captive and wild situations has led to an i
Authors
Elizabeth A. Bohuski, Jeffrey M. Lorch, Kathryn M. Griffin, David S. Blehert
Gnathostoma spinigerum in live Asian swamp eels (Monopterus spp.) from food markets and wild populations, United States
In Southeast Asia, swamp eels (Synbranchidae: Monopterus spp.) are a common source of human gnathostomiasis, a foodborne zoonosis caused by advanced third-stage larvae (AL3) of Gnathostoma spp. nematodes. Live Asian swamp eels are imported to US ethnic food markets, and wild populations exist in several states. To determine whether these eels are infected, we examined 47 eels from markets and 67 w
Authors
Rebecca A. Cole, Anindo Choudhury, Leo G. Nico, Kathryn M. Griffin
Changes in West Nile virus seroprevalence and antibody titers among Wisconsin mesopredators 2003-2006
After the 2001 occurrence of West Nile virus (WNV) in Wisconsin (WI), we collected sera, during 2003–2006, from south-central WI mesopredators. We tested these sera to determine WNV antibody prevalence and geometric mean antibody titer (GMAT). Four-fold higher antibody prevalence and 2-fold higher GMAT in 2003–2004 indicated greater exposure of mesopredators to WNV during the apparent epizootic ph
Authors
Douglas E. Docherty, Michael D. Samuel, Kristina F. Egstad, Kathryn M. Griffin, Cherrie A. Nolden, L. Karwal, Hon S. Ip
West Nile virus antibody prevalence in wild mammals, southern Wisconsin
Twenty percent prevalence of West Nile virus antibody was found in free-ranging medium-sized Wisconsin mammals. No significant differences were noted in antibody prevalence with regard to sex, age, month of collection, or species. Our results suggest a similar route of infection in these mammals.
Authors
Douglas E. Docherty, Michael D. Samuel, C.A. Nolden, Kristina F. Egstad, Kathryn M. Griffin
Science and Products
Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America”
In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, raccoons, and skunks, 261 (72%) of the animals harbored a
Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction Reagent. RHDV2
Authors
Erik K. Hofmeister, Kathryn M. Griffin, Hon S. Ip
An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America
In summer 2020, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from on or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, racc
Authors
Hon S. Ip, Kathryn M. Griffin, Jeffrey D. Messer, Megan Winzeler, Susan A. Shriner, Mary Lea Killian, Mia K. Torchetti, Thomas J. DeLiberto, Brian R. Amman, Caitlin M. Cossaboom, R. Reid Harvey, Natalie M. Wendling, Hannah Rettler, Dean Taylor, Jonathan S. Towner, Casey Barton Behravesh, David S. Blehert
First detection of bat white-nose syndrome in western North America
White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia to naive ho
Authors
Jeffrey M. Lorch, Jonathan M. Palmer, Daniel L. Lindner, Anne Ballmann, Kyle George, Kathryn M. Griffin, Susan Knowles, John R. Huckabee, Katherine H. Haman, Christopher D. Anderson, Penny A. Becker, Joseph B. Buchanan, Jeffrey T. Foster, David S. Blehert
TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease
Background
Fungal skin infections associated with Ophidiomyces ophiodiicola, a member of the Chrysosporiumanamorph of Nannizziopsis vriesii (CANV) complex, have been linked to an increasing number of cases of snake fungal disease (SFD) in captive snakes around the world and in wild snake populations in eastern North America. The emergence of SFD in both captive and wild situations has led to an i
Authors
Elizabeth A. Bohuski, Jeffrey M. Lorch, Kathryn M. Griffin, David S. Blehert
Gnathostoma spinigerum in live Asian swamp eels (Monopterus spp.) from food markets and wild populations, United States
In Southeast Asia, swamp eels (Synbranchidae: Monopterus spp.) are a common source of human gnathostomiasis, a foodborne zoonosis caused by advanced third-stage larvae (AL3) of Gnathostoma spp. nematodes. Live Asian swamp eels are imported to US ethnic food markets, and wild populations exist in several states. To determine whether these eels are infected, we examined 47 eels from markets and 67 w
Authors
Rebecca A. Cole, Anindo Choudhury, Leo G. Nico, Kathryn M. Griffin
Changes in West Nile virus seroprevalence and antibody titers among Wisconsin mesopredators 2003-2006
After the 2001 occurrence of West Nile virus (WNV) in Wisconsin (WI), we collected sera, during 2003–2006, from south-central WI mesopredators. We tested these sera to determine WNV antibody prevalence and geometric mean antibody titer (GMAT). Four-fold higher antibody prevalence and 2-fold higher GMAT in 2003–2004 indicated greater exposure of mesopredators to WNV during the apparent epizootic ph
Authors
Douglas E. Docherty, Michael D. Samuel, Kristina F. Egstad, Kathryn M. Griffin, Cherrie A. Nolden, L. Karwal, Hon S. Ip
West Nile virus antibody prevalence in wild mammals, southern Wisconsin
Twenty percent prevalence of West Nile virus antibody was found in free-ranging medium-sized Wisconsin mammals. No significant differences were noted in antibody prevalence with regard to sex, age, month of collection, or species. Our results suggest a similar route of infection in these mammals.
Authors
Douglas E. Docherty, Michael D. Samuel, C.A. Nolden, Kristina F. Egstad, Kathryn M. Griffin