Marshal Hoy
Biography
Education:
B.S. Botany, University of Washington, Seattle, WA
Research Interests:
I am a member of the Ecology Section at the U.S. Geological Survey's Western Fisheries Research Center in Seattle, WA. I have participated in several projects using environmental DNA (eDNA) technology, which includes involvement in the entire process from molecular assay design, field sampling and laboratory processing/analysis. We have used eDNA based methods to:
- Monitor recolonization, distribution and migration of fish species
- Provide abundance/biomass/density estimates
- Quantification of biodiversity in Marine communities.
I am also involved in many other exciting projects such as the genetic analysis of several fish species (including the development of genetic species marker for the endangered Lost River and shortnose suckers in the Klamath Basin), using food web interactions and bioenergetics to study juvenile salmon survival in Puget Sound, and USGS Elwha River nearshore long-term dam removal monitoring project.
Experience:
4/2009 to Present- Biological Science Laboratory Technician, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
2002 - 4/2009- Research Scientist, University of Washington, Seattle, WA
Honors, Awards, Recognition, Elected Memberships:
1/2010 - Star Award, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
Science and Products
Concentrations of environmental DNA (eDNA) during sockeye salmon spawning in 2016, Hansen Creek, Alaska, USA
This dataset contains environmental DNA (eDNA) concentrations and spawning adult abundances for sockeye salmon (Oncorhynchus nerka) in Hansen Creek, a small tributary in the Wood River watershed, southeast Alaska, USA. We took temporally- and spatially-replicated eDNA samples during the spawning period (mid-July through August), along with fish counts (live, naturally dead
Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America
We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these...
Hoy, Marshal; Ostberg, Carl Evaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed
Surveys of environmental DNA (eDNA) have become an important and multifaceted tool for monitoring and identifying distributions and occupancy of aquatic species. This tool is attractive because it is powerful, easy to apply, and provides an alternative to traditional field survey methods. However, validating eDNA survey methods against traditional...
Ostberg, Carl; Chase, Dorothy Murphy; Hoy, Marshal; Duda, Jeff; Hayes, Michael; Jolley, Jeffrey; Silver, Gregory S; Cook-Tabor, Carrie
Juvenile Lost River and shortnose sucker year class strength, survival, and growth in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2016 Monitoring Report
Executive SummaryThe largest populations of federally endangered Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) exist in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California. Upper Klamath Lake populations are decreasing because adult mortality, which is relatively low, is not being balanced by...
Burdick, Summer M.; Ostberg, Carl O.; Hoy, Marshal S. Concentrations of environmental DNA (eDNA) reflect spawning salmon abundance at fine spatial and temporal scales
Developing fast, cost-effective assessments of wild animal abundance is an important goal for many researchers, and environmental DNA (eDNA) holds much promise for this purpose. However, the quantitative relationship between species abundance and the amount of DNA present in the environment is likely to vary substantially among taxa and with...
Tillotson, Michael D.; Kelly, Ryan P.; Duda, Jeffrey J.; Hoy, Marshal S.; Kralj, James; Quinn, Thomas P.
Health and condition of endangered young-of-the-year Lost River and Shortnose suckers relative to water quality in Upper Klamath Lake, Oregon, 2014–2015
Most mortality of endangered Lost River (Deltistes luxatus) and shortnose (Chasmistes brevirostris) suckers in Upper Klamath Lake, Oregon, occurs within the first year of life. Juvenile suckers in Clear Lake Reservoir, California, survive longer and may even recruit to the spawning populations. In a previous (2013–2014) study, the health and...
Burdick, Summer M.; Conway, Carla M.; Elliott, Diane G.; Hoy, Marshal S.; Dolan-Caret, Amari; Ostberg, Carl O.
Juvenile sucker cohort tracking data summary and assessment of monitoring program, 2015
Populations of federally endangered Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, are experiencing long-term declines in abundance. Upper Klamath Lake populations are decreasing because adult mortality, which is relatively low, is not being balanced by recruitment of young adult...
Burdick, Summer M.; Ostberg, Carl O.; Hereford, Mark E.; Hoy, Marshal S.
Health and condition of endangered juvenile Lost River and shortnose suckers relative to water quality and fish assemblages in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California
Executive Summary Most mortality of endangered Lost River (Deltistes luxatus) and shortnose (Chasmistes brevirostris) suckers in Upper Klamath Lake, Oregon, appears to occur within the first year of life. However, juvenile suckers in Clear Lake Reservoir, California, appear to survive longer and may even recruit to the spawning populations. Our...
Burdick, Summer M.; Elliott, Diane G.; Ostberg, Carl O.; Conway, Carla M.; Dolan-Caret, Amari; Hoy, Marshal S.; Feltz, Kevin P.; Echols, Kathy R. Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers
Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker (Chasmistes brevirostris) and Lost River sucker (Deltistes luxatus). Here, we developed 20 Taqman assays that...
Hoy, Marshal S.; Ostberg, Carl O. Intragenomic sequence variation at the ITS1 - ITS2 region and at the 18S and 28S nuclear ribosomal DNA genes of the New Zealand mud snail, Potamopyrgus antipodarum (Hydrobiidae: mollusca)
Molecular genetic analysis was conducted on two populations of the invasive non-native New Zealand mud snail (Potamopyrgus antipodarum), one from a freshwater ecosystem in Devil's Lake (Oregon, USA) and the other from an ecosystem of higher salinity in the Columbia River estuary (Hammond Harbor, Oregon, USA). To elucidate potential genetic...
Hoy, Marshal S.; Rodriguez, Rusty J. Salinity adaptation of the invasive New Zealand mud snail (Potamopyrgus antipodarum) in the Columbia River estuary (Pacific Northwest, USA): Physiological and molecular studies
In this study, we examine salinity stress tolerances of two populations of the invasive species New Zealand mud snail Potamopyrgus antipodarum, one population from a high salinity environment in the Columbia River estuary and the other from a fresh water lake. In 1996, New Zealand mud snails were discovered in the tidal reaches of the Columbia...
Hoy, Marshal; Boese, Bruce L.; Taylor, Louise; Reusser, Deborah; Rodriguez, Rusty Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific...
Hoy, M.S.; Kelly, K.; Rodriguez, R.J. Molecular detection of native and invasive marine invertebrate larvae present in ballast and open water environmental samples collected in Puget Sound
Non-native marine species have been and continue to be introduced into Puget Sound via several vectors including ship's ballast water. Some non-native species become invasive and negatively impact native species or near shore habitats. We present a new methodology for the development and testing of taxon specific PCR primers designed to assess...
Harvey, J.B.J.; Hoy, M.S.; Rodriguez, R.J.