Shaun Baesman
Shaun Baesman is a Biologist for the USGS Water Resources Mission Area.
Science and Products
Major, Minor, and Trace Element Results from Sequential Extractions of Sediment Cores Collected from the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, 2014 to 2019 Major, Minor, and Trace Element Results from Sequential Extractions of Sediment Cores Collected from the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, 2014 to 2019
This U.S. Geological Survey (USGS) Data Release contains chemical analysis information on sediment core and oil samples collected at the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, USA. Following a crude-oil spill in 1979, biodegradation of petroleum hydrocarbons in the contaminated aquifer has formed distinct zones of pH and/or redox...
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93 Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene...
Authors
Denise M. Akob, Shaun Baesman, John M. Sutton, Janna L. Fierst, Adam C. Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy C. Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia
Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C2H2 is known to inhibit bacterial dechlorination. In this study, we show that while high C2H2 (1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by Dehalococcoides mccartyi isolates as well as enrichment...
Authors
Xinwei Mao, Ronald S. Oremland, Tong Liu, Abigail A Landers, Shaun Baesman, Lisa Alvarez-Cohen
Stable carbon isotope fractionation during bacterial acetylene fermentation: Potential for life detection in hydrocarbon-rich volatiles of icy planet(oid)s Stable carbon isotope fractionation during bacterial acetylene fermentation: Potential for life detection in hydrocarbon-rich volatiles of icy planet(oid)s
We report the first study of stable carbon isotope fractionation during microbial fermentation of acetylene (C2H2) in sediments, sediment enrichments, and bacterial cultures. Kinetic isotope effects (KIEs) averaged 3.7 ± 0.5‰ for slurries prepared with sediment collected at an intertidal mudflat in San Francisco Bay and 2.7 ± 0.2‰ for a pure culture of Pelobacter sp. isolated from these...
Authors
Laurence Miller, Shaun Baesman, Ron Oremland
Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment
The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO2 with some CH4 present. The δ13CH4 value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively 12C...
Authors
Shaun Baesman, Laurence G. Miller, Jeremy H. Wei, Yirang Cho, Emily D. Matys, Roger E. Summons, Paula V. Welander, Ronald S. Oremland
Methane oxidation linked to chlorite dismutation Methane oxidation linked to chlorite dismutation
We examined the potential for CH4 oxidation to be coupled with oxygen derived from the dissimilatory reduction of perchlorate, chlorate, or via chlorite (ClO−2) dismutation. Although dissimilatory reduction of ClO−4 and ClO−3 could be inferred from the accumulation of chloride ions either in spent media or in soil slurries prepared from exposed freshwater lake sediment, neither of these...
Authors
Laurence G. Miller, Shaun M. Baesman, Charlotte I. Carlstrom, John D. Coates, Ronald S. Oremland
A biogeochemical and genetic survey of acetylene fermentation by environmental samples and bacterial isolates A biogeochemical and genetic survey of acetylene fermentation by environmental samples and bacterial isolates
Anoxic samples (sediment and groundwater) from 13 chemically diverse field sites were assayed for their ability to consume acetylene (C2H2). Over incubation periods ranging from ˜ 10 to 80 days, selected samples from 7 of the 13 tested sites displayed significant C2H2 removal. No significant formation of ethylene was noted in these incubations; therefore, C2H2 consumption could be...
Authors
Laurence G. Miller, Shaun M. Baesman, Julie Kirshtein, Mary A. Voytek, Ronald S. Oremland
Science and Products
Major, Minor, and Trace Element Results from Sequential Extractions of Sediment Cores Collected from the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, 2014 to 2019 Major, Minor, and Trace Element Results from Sequential Extractions of Sediment Cores Collected from the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, 2014 to 2019
This U.S. Geological Survey (USGS) Data Release contains chemical analysis information on sediment core and oil samples collected at the National Crude Oil Spill Fate and Natural Attenuation Research Site near Bemidji, Minnesota, USA. Following a crude-oil spill in 1979, biodegradation of petroleum hydrocarbons in the contaminated aquifer has formed distinct zones of pH and/or redox...
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93 Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene...
Authors
Denise M. Akob, Shaun Baesman, John M. Sutton, Janna L. Fierst, Adam C. Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy C. Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia
Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C2H2 is known to inhibit bacterial dechlorination. In this study, we show that while high C2H2 (1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by Dehalococcoides mccartyi isolates as well as enrichment...
Authors
Xinwei Mao, Ronald S. Oremland, Tong Liu, Abigail A Landers, Shaun Baesman, Lisa Alvarez-Cohen
Stable carbon isotope fractionation during bacterial acetylene fermentation: Potential for life detection in hydrocarbon-rich volatiles of icy planet(oid)s Stable carbon isotope fractionation during bacterial acetylene fermentation: Potential for life detection in hydrocarbon-rich volatiles of icy planet(oid)s
We report the first study of stable carbon isotope fractionation during microbial fermentation of acetylene (C2H2) in sediments, sediment enrichments, and bacterial cultures. Kinetic isotope effects (KIEs) averaged 3.7 ± 0.5‰ for slurries prepared with sediment collected at an intertidal mudflat in San Francisco Bay and 2.7 ± 0.2‰ for a pure culture of Pelobacter sp. isolated from these...
Authors
Laurence Miller, Shaun Baesman, Ron Oremland
Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment
The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO2 with some CH4 present. The δ13CH4 value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively 12C...
Authors
Shaun Baesman, Laurence G. Miller, Jeremy H. Wei, Yirang Cho, Emily D. Matys, Roger E. Summons, Paula V. Welander, Ronald S. Oremland
Methane oxidation linked to chlorite dismutation Methane oxidation linked to chlorite dismutation
We examined the potential for CH4 oxidation to be coupled with oxygen derived from the dissimilatory reduction of perchlorate, chlorate, or via chlorite (ClO−2) dismutation. Although dissimilatory reduction of ClO−4 and ClO−3 could be inferred from the accumulation of chloride ions either in spent media or in soil slurries prepared from exposed freshwater lake sediment, neither of these...
Authors
Laurence G. Miller, Shaun M. Baesman, Charlotte I. Carlstrom, John D. Coates, Ronald S. Oremland
A biogeochemical and genetic survey of acetylene fermentation by environmental samples and bacterial isolates A biogeochemical and genetic survey of acetylene fermentation by environmental samples and bacterial isolates
Anoxic samples (sediment and groundwater) from 13 chemically diverse field sites were assayed for their ability to consume acetylene (C2H2). Over incubation periods ranging from ˜ 10 to 80 days, selected samples from 7 of the 13 tested sites displayed significant C2H2 removal. No significant formation of ethylene was noted in these incubations; therefore, C2H2 consumption could be...
Authors
Laurence G. Miller, Shaun M. Baesman, Julie Kirshtein, Mary A. Voytek, Ronald S. Oremland