Yer Lor
Yer Lor
Upper Midwest Environmental Sciences Center
Science and Products
The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data
Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, data and metadata makes results difficult to reproduce and synthesise. A working group of scientists have collaborated to produce a set of minimum reporting guidelines for the constituent steps of metabarcoding...
Authors
Katy E. Klymus, Jacoby D. Baker, Cathryn L. Abbott, Rachel J. Brown, Joseph M. Craine, Zachary Gold, Margaret Hunter, Mark D. Johnson, Devin Nicole Jones, Michelle J. Jungbluth, Sean P. Jungbluth, Yer Lor, Aaron P. Maloy, Christopher M. Merkes, Rachel T. Noble, Nastassia V. Patin, Adam Sepulveda, Stephen Frank Spear, Joshua A. Steele, Miwa Takahashi, Alison W. Watts, Susanna Theroux
Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
Insufficient water sample volumes can be a limiting factor for detecting species with environmental DNA (eDNA) from aquatic habitats. We compared detections of freshwater mussel (Unionidae) communities using large water sample volumes and dead-end hollow fiber ultrafiltration (D-HFUF or DEUF) with traditional eDNA filtration methods that use relatively small water sample volumes. Unionid...
Authors
Anna M. McKee, Katy E. Klymus, Yer Lor, Marissa H Kaminski, Tariq Tajjioui, Nathan Johnson, Matthew Carroll, Christopher Goodson, Stephen Frank Spear
The relative importance of cisco (Coregonus artedi) in the diets of common loons (Gavia immer) among a set of cisco refuge lakes in Minnesota
Common loon (Gavia immer (Brünnich, 1764)) foraging patterns and the relative importance of cisco (Coregonus artedi Lesueur, 1818) in the diets of loons were evaluated for the Whitefish Chain of Lakes, a set of coldwater cisco refuge lakes in Minnesota, USA. Environmental DNA metabarcoding of loon fecal samples detected 15 fish species. Yellow perch (Perca flavescens (Mitchill, 1814))...
Authors
Kevin P. Kenow, Yer Lor, Beth V. Holbrook, Luke J. Fara, Steven C. Houdek, Tariq Tajjioui, Brian R. Gray, Peter C. Jacobson
Environmental DNA metabarcoding as a tool for biodiversity assessment and monitoring: Reconstructing established fish communities of north-temperate lakes and rivers
AimTo evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on conventional field sampling, 2) compare efficiency of...
Authors
Peter T. Euclide, Yer Lor, Michael J. Spear, Tariq Tajjioui, Jake Vander Zanden, Wesley Larson, Jon Amberg
Using environmental DNA (eDNA) to detect the endangered Spectaclecase Mussel (Margaritifera monodonta)
Margaritifera monodonta, or the Spectaclecase Mussel, is a federally endangered freshwater mussel species that has experienced a 55% reduction in range and is currently concentrated in 3 rivers in the Midwest region of the United States (Gasconade and Meramec rivers, Missouri, and St Croix River, Wisconsin). The detection of new populations by traditional survey methods has been limited...
Authors
Yer Lor, Theresa M. Schreier, Diane L. Waller, Christopher M. Merkes
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published...
Environmental DNA metabarcoding results of fish prey DNA in common loon (Gavia immer) fecal and cloacal swab samples from the Whitefish Chain of Lakes, Crow Wing County, Minnesota
The dataset contains sequencing read counts of fish prey eDNA metabarcoding (using primers targeting the 16S rRNA mitochondrial gene) that were extracted from fecal and cloacal swab samples collected from common loons (Gavia immer) captured on the Whitefish Chain of Lakes, Crow Wing County, Minnesota during 2015-2106. Sample type (cloacal or fecal); loon identification, age, and sex...
Science and Products
The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data
Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, data and metadata makes results difficult to reproduce and synthesise. A working group of scientists have collaborated to produce a set of minimum reporting guidelines for the constituent steps of metabarcoding...
Authors
Katy E. Klymus, Jacoby D. Baker, Cathryn L. Abbott, Rachel J. Brown, Joseph M. Craine, Zachary Gold, Margaret Hunter, Mark D. Johnson, Devin Nicole Jones, Michelle J. Jungbluth, Sean P. Jungbluth, Yer Lor, Aaron P. Maloy, Christopher M. Merkes, Rachel T. Noble, Nastassia V. Patin, Adam Sepulveda, Stephen Frank Spear, Joshua A. Steele, Miwa Takahashi, Alison W. Watts, Susanna Theroux
Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
Insufficient water sample volumes can be a limiting factor for detecting species with environmental DNA (eDNA) from aquatic habitats. We compared detections of freshwater mussel (Unionidae) communities using large water sample volumes and dead-end hollow fiber ultrafiltration (D-HFUF or DEUF) with traditional eDNA filtration methods that use relatively small water sample volumes. Unionid...
Authors
Anna M. McKee, Katy E. Klymus, Yer Lor, Marissa H Kaminski, Tariq Tajjioui, Nathan Johnson, Matthew Carroll, Christopher Goodson, Stephen Frank Spear
The relative importance of cisco (Coregonus artedi) in the diets of common loons (Gavia immer) among a set of cisco refuge lakes in Minnesota
Common loon (Gavia immer (Brünnich, 1764)) foraging patterns and the relative importance of cisco (Coregonus artedi Lesueur, 1818) in the diets of loons were evaluated for the Whitefish Chain of Lakes, a set of coldwater cisco refuge lakes in Minnesota, USA. Environmental DNA metabarcoding of loon fecal samples detected 15 fish species. Yellow perch (Perca flavescens (Mitchill, 1814))...
Authors
Kevin P. Kenow, Yer Lor, Beth V. Holbrook, Luke J. Fara, Steven C. Houdek, Tariq Tajjioui, Brian R. Gray, Peter C. Jacobson
Environmental DNA metabarcoding as a tool for biodiversity assessment and monitoring: Reconstructing established fish communities of north-temperate lakes and rivers
AimTo evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on conventional field sampling, 2) compare efficiency of...
Authors
Peter T. Euclide, Yer Lor, Michael J. Spear, Tariq Tajjioui, Jake Vander Zanden, Wesley Larson, Jon Amberg
Using environmental DNA (eDNA) to detect the endangered Spectaclecase Mussel (Margaritifera monodonta)
Margaritifera monodonta, or the Spectaclecase Mussel, is a federally endangered freshwater mussel species that has experienced a 55% reduction in range and is currently concentrated in 3 rivers in the Midwest region of the United States (Gasconade and Meramec rivers, Missouri, and St Croix River, Wisconsin). The detection of new populations by traditional survey methods has been limited...
Authors
Yer Lor, Theresa M. Schreier, Diane L. Waller, Christopher M. Merkes
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published...
Environmental DNA metabarcoding results of fish prey DNA in common loon (Gavia immer) fecal and cloacal swab samples from the Whitefish Chain of Lakes, Crow Wing County, Minnesota
The dataset contains sequencing read counts of fish prey eDNA metabarcoding (using primers targeting the 16S rRNA mitochondrial gene) that were extracted from fecal and cloacal swab samples collected from common loons (Gavia immer) captured on the Whitefish Chain of Lakes, Crow Wing County, Minnesota during 2015-2106. Sample type (cloacal or fecal); loon identification, age, and sex...