Publications

Determination of PCBs in biological tissue extracts by enzyme-linked immunosorbent assays (ELISAs) can be problematic, since the hydrophobic solvents used for their extraction and isolation from interfering biochemicals have limited compatibility with the polar solvents (e.g. methanol/water) and the immunochemical reagents used in ELISA. Our studies of these solvent effects indicate that

Behavioral functions essential for growth and survival can be dramatically altered by sublethal exposure to toxicants. Measures of these behavioral responses are effective in detecting adverse effects of sublethal contaminant exposure. Behavioral responses of fishes can be qualitatively and quantitatively evaluated during routine toxicity tests. At selected intervals of exposure...

Natural Resource Damage Assessment (NRDA) provisions enacted under Comprehensive Environmental Response Compensation and Liability Act (CERCLA) and the Oil Pollution Act (OPA) empower natural resource trustees to seek compensation for environmental injury resulting from the release of oil or hazardous substances. Under NRDA regulations promulgated under CERCLA, fish avoidance behavior is

The yolks of White Leghorn chicken (Gallus domesticus) eggs were injected prior to incubation with either 3,3′,4,4′,5- pentachlorobiphenyl (PCB 126) at doses ranging from 0.1 to 12.8 μg/kg egg or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at doses ranging from 0.04 to 0.64 μg/kg egg. Chicks were subjected to necropsy within 24 h of hatching. The brain, bursa, heart, liver, and spleen...

The Water Quality Act of 1987 required the U.S. Environmental Protection Agency to regulate stormwater discharges under the National Pollutant Discharge Elimination System program, and guidelines for obtaining permits under this program were established for areas served by municipal separate storm sewer systems with populations greater than 100,000 (U.S. Environmental Protection Agency...

No abstract available.

We determined fluxes of oxygen and nutrients between water and sediments at 21 sites primarily in Virginia and North Carolina estuaries, over the past 15 yr. These sites represented broad ranges in salinity, tidal amplitude, hydrology, nutrient availability, turbidity, light availability, depth, sediment grain size, and anthropogenic disturbance. In general, we found that...

To characterize the quality of sediments at key sites in the Galveston Bay Estuary, sediment samples were collected concurrently for chemical and physical analyses, toxicity testing and an assessment of benthic community structure. Significant toxicity, as determined by the sea urchin (Arbacia punetulata) pore water embryological development assay, was observed at 12 of the 24 sites...

Persistent Hydrophobic contaminants such as poly chlorinated dibenzo-p-dioxins, dibenzofurans and biphenyl congeners are present in aquatic systems, and are known to produce adverse effects in fish. Reproductive failure in fish populations has been observed in aquatic systems contaminated with persistent hydrophobic compounds. In order to mimic maternal transfer of environmental...

A 41.3-kg sample of double-crested cormorant (Phalacrocorax auritus) egg contents was extracted, yielding over 2 L of egg lipid. The double-crested cormorant (DCC) egg extract, after clean-up and concentration, was intended for use in egg injection studies to determine the embryotoxicity of the organic contaminants found within the eggs. Large-scale dialysis was used as a preliminary...

Food-web components of a Lake Superior coastal wetland and adjacent offshore waters were examined with stable isotope ratio techniques for carbon and nitrogen. We found distinct carbon isotope ratio signatures for organisms collected in the wetland and from offshore. Both food-web groups seemed to be based on carbon fixed by phytoplankton. Compared to offshore organisms, the wetland food...

This paper describes a new approach using decision support GIS for handling information in ecosystem management. Technical research efforts resulted in specialized spatial decision support systems for wetland restoration planning, wetland permit analysis, and wildlife research and management. These applications are briefly presented to illustrate the usage of the methodology. With its...