Publications
USGS divers in the Elwha River
WFRC zebrafish laboratory
Processing a Lost River sucker
Below is a list of available WFRC peer reviewed and published science.
Filter Total Items: 2525
An experimental vaccine against Aeromonas hydrophila can induce protection in rainbow trout, Oncorhynchus mykiss (Walbaum) An experimental vaccine against Aeromonas hydrophila can induce protection in rainbow trout, Oncorhynchus mykiss (Walbaum)
A candidate vaccine against Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss, was developed using a bacterial lysate. To test the strength of protection, A. hydrophila challenge models were compared using injection into both the intraperitoneal (IP) cavity and the dorsal sinus (DS) with selected doses of live bacteria washed in saline or left untreated. Unlike the IP route...
Authors
S. E. LaPatra, K.P. Plant, S. Alcorn, V. Ostland, J. Winton
Diel behavior of rearing fall Chinook salmon Diel behavior of rearing fall Chinook salmon
In fisheries science, habitat use is often inferred when fish are sampled or observed in a particular location. Physical habitat is typically measured where fish are found, and thus deemed important to habitat use. Although less common, a more informative approach is to measure or observe fish behavior within given habitats to more thoroughly assess their use of those locations. While...
Authors
Kenneth F. Tiffan, Tobias J. Kock, Joseph J. Skalicky
Pathological and immunological responses associated with differential survival of Chinook salmon following Renibacterium salmoninarum challenge Pathological and immunological responses associated with differential survival of Chinook salmon following Renibacterium salmoninarum challenge
Chinook salmon Oncorhynchus tshawytscha are highly susceptible to Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD). Previously we demonstrated that introduced Chinook salmon from Lake Michigan, Wisconsin (WI), USA, have higher survival following R. salmoninarum challenge relative to the progenitor stock from Green River, Washington, USA. In the present...
Authors
David C. Metzger, Diane G. Elliott, Andrew Wargo, Linda K. Park, Maureen K. Purcell
Minimizing effects of over-water docks on federally listed fish stocks in McNary Reservoir: A literature review for criteria Minimizing effects of over-water docks on federally listed fish stocks in McNary Reservoir: A literature review for criteria
McNary Lock and Dam were completed in 1953, creating McNary Reservoir, or Lake Wallula. The shoreline of the reservoir is federally owned and as a result the U.S. Army Corps of Engineers (USACE) has certain land and fish habitat management responsibilities to balance with other multipurpose benefits. The Endangered Species Act (ESA) listing of Columbia and Snake River salmon stocks has...
Authors
Dennis W. Rondorf, Gary L. Rutz, Jodi C. Charrier
Guidelines to indirectly measure and enhance detection efficiency of stationary PIT tag interrogation systems in streams Guidelines to indirectly measure and enhance detection efficiency of stationary PIT tag interrogation systems in streams
With increasing use of passive integrated transponder (PIT) tags and reliance on stationary PIT tag interrogation systems to monitor fish populations, guidelines are offered to inform users how best to use limited funding and human resources to create functional systems that maximize a desired level of detection and precision. The estimators of detection efficiency and their variability...
Authors
Patrick J. Connolly
Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout–derived cell line, RTG-2 Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout–derived cell line, RTG-2
Infectious haematopoietic necrosis virus (IHNV) is one of the most important viral pathogens of salmonids. In rainbow trout, IHNV isolates in the M genogroup are highly pathogenic, while U genogroup isolates are significantly less pathogenic. We show here that, at a multiplicity of infection (MOI) of 1, a representative U type strain yielded 42‐fold less infectious virus than an M type...
Authors
Jeong Woo Park, Chang Hoon Moon, Andrew Wargo, Maureen K. Purcell, Gael Kurath
Quantifying the behavioral response of spawning chum salmon to elevated discharges from Bonneville Dam, Columbia River, USA Quantifying the behavioral response of spawning chum salmon to elevated discharges from Bonneville Dam, Columbia River, USA
Chum salmon Oncorhynchus keta that spawn in main-stem habitats below Bonneville Dam on the Columbia River, USA, are periodically subjected to elevated discharges that may alter spawning behaviour. We investigated behavioural responses of spawning chum salmon to increased water velocities associated with experimental increases in tailwater elevation using acoustic telemetry and a dual...
Authors
K.F. Tiffan, C. A. Haskell, T.J. Kock
Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel) Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the...
Authors
M.S. Hoy, K. Kelly, R. J. Rodriguez
Annual sex steroid and other physiological profiles of Pacific lampreys (Entosphenus tridentatus) Annual sex steroid and other physiological profiles of Pacific lampreys (Entosphenus tridentatus)
We documented changes in plasma levels of estradiol 17-β (E2), progesterone (P), 15α-hydroxytestosterone (15α-T), thyroxine (T4), triiodothyronine (T3), protein, triglycerides (TGs), and glucose in adult Pacific lampreys (Entosphenus tridentatus) held in the laboratory in two different years. Levels of E2 in both sexes ranged from 0.5 to 2 ng/mL from September to March, peaked in late...
Authors
Matthew G. Mesa, Jennifer M. Bayer, Mara B. Bryan, Stacia A. Sower
Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss) Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss)
Induction of the innate immune pathways is critical for early anti-viral defense but there is limited understanding of how teleost fish recognize viral molecules and activate these pathways. In mammals, Toll-like receptors (TLR) 7 and 8 bind single-stranded RNA of viral origin and are activated by synthetic anti-viral imidazoquinoline compounds. Herein, we identify and describe the...
Authors
Yniv Palti, Scott A. Gahr, Maureen K. Purcell, Sima Hadidi, Caird E. Rexroad, Gregory A. Wiens
Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica
During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales...
Authors
Russell J. Rodriguez, L. Connell, R. Redman, A. Barrett, M. Iszard, A. Fonseca