Skip to main content
U.S. flag

An official website of the United States government

Publications

Below is a list of available WFRC peer reviewed and published science.

Filter Total Items: 2562

Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV) Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV)

The main objective of this study was to assess correlates of innate resistance in rainbow trout full-sibling families that differ in susceptibility to Infectious hematopoietic necrosis virus (IHNV). As part of a commercial breeding program, full-sibling families were challenged with IHNV by waterborne exposure at the 1 g size to determine susceptibility to IHNV. Progeny from select...
Authors
M. K. Purcell, S. E. LaPatra, J.C. Woodson, Gael Kurath, J. R. Winton

Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel) Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)

A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the...
Authors
M.S. Hoy, K. Kelly, R. J. Rodriguez

Faunal assemblages and multi-scale habitat patterns in headwater tributaries of the South Fork Trinity River - an unregulated river embedded within a multiple-use landscape Faunal assemblages and multi-scale habitat patterns in headwater tributaries of the South Fork Trinity River - an unregulated river embedded within a multiple-use landscape

Headwaters can represent 80% of stream kilometers in a watershed, and they also have unique physical and biological properties that have only recently been recognized for their importance in sustaining healthy functioning stream networks and their ecological services. We sampled 60 headwater tributaries in the South Fork Trinity River, a 2,430 km2, mostly forested, multiple-use watershed...
Authors
H.H. Welsh, G.R. Hodgson, J.J. Duda, J.M. Emlen

Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells

Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, Bearzotti, Muzinic, Zwillenberg, Chilmonczyk, Vautherot & de Kinkelin 1983) has become one of the most widely used tools for research on fish viruses and the diagnosis of fish viral diseases.
Authors
J. Winton, W. Batts, P. DeKinkelin, M. LeBerre, M. Bremont, N. Fijan

Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss) Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss)

Induction of the innate immune pathways is critical for early anti-viral defense but there is limited understanding of how teleost fish recognize viral molecules and activate these pathways. In mammals, Toll-like receptors (TLR) 7 and 8 bind single-stranded RNA of viral origin and are activated by synthetic anti-viral imidazoquinoline compounds. Herein, we identify and describe the...
Authors
Yniv Palti, Scott A. Gahr, Maureen K. Purcell, Sima Hadidi, Caird E. Rexroad, Gregory A. Wiens

Improving inferences from fisheries capture-recapture studies through remote detection of PIT tags Improving inferences from fisheries capture-recapture studies through remote detection of PIT tags

Models for capture-recapture data are commonly used in analyses of the dynamics of fish and wildlife populations, especially for estimating vital parameters such as survival. Capture-recapture methods provide more reliable inferences than other methods commonly used in fisheries studies. However, for rare or elusive fish species, parameter estimation is often hampered by small...
Authors
David A. Hewitt, Eric C. Janney, Brian S. Hayes, Rip S. Shively

Amplification and transport of an endemic fish disease by an introduced species Amplification and transport of an endemic fish disease by an introduced species

The introduction of American shad from the Atlantic to the Pacific coast of North America in the late 1800’s and the subsequent population expansion in the 1980’s resulted in the amplification of Ichthyophonus sp., a Mesomycetozoean parasite of wild marine fishes. Sequence analysis of the ribosomal DNA gene complex (small subunit and internal transcribed spacer regions) and Ichthyophonus
Authors
Paul Hershberger, Bjorn Leeuw, Gregg Jacob, Courtney Grady, Kenneth Lujan, Susan Gutenberger, Maureen K. Purcell, James Woodson, James Winton, Michael Parsley

Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales...
Authors
Russell J. Rodriguez, L. Connell, R. Redman, A. Barrett, M. Iszard, A. Fonseca

Transcriptome analysis of rainbow trout infected with high and low virulence strains of Infectious hematopoietic necrosis virus Transcriptome analysis of rainbow trout infected with high and low virulence strains of Infectious hematopoietic necrosis virus

There are three main genetic lineages or genogroups of Infectious hematopoietic necrosis virus (IHNV) in N. America. Strains representing the M genogroup are more virulent in rainbow trout relative to the U genogroup. In this study, we used microarray analysis to evaluate potential mechanisms responsible for host-specific virulence in rainbow trout that were given intraperitoneal...
Authors
Maureen K. Purcell, Inderjit Singh Marjara, William Batts, Gael Kurath, John D. Hansen

Impacts of climate change on Oregon's coasts and estuaries Impacts of climate change on Oregon's coasts and estuaries

Earth’s changing climate is expected to have significant physical impacts along the coast and estuarine shorelands of Oregon, ranging from increased erosion and inundation of low lying areas, to wetland loss and increased estuarine salinity. The environmental changes associated with climate change include rising sea levels, increased occurrences of severe storms, rising air and water...
Authors
Ruggiero, Cheryl A. Brown, Paul D. Komar, Jonathan C. Allan, Deborah A. Reusser, Henry Lee

Feasibility of Surgically Implanting Acoustic Tags into Pacific Herring Feasibility of Surgically Implanting Acoustic Tags into Pacific Herring

Internally implanted acoustic tags represent a potentially valuable approach to assessing the seasonal migration and distribution patterns of Pacific herring Clupea palasii. We examined the feasibility of implanting two sizes of dummy acoustic tags (9 mm in diameter × 21 mm long, 1.6 g; and 7 mm in diameter × 18 mm long, 0.7 g) in Pacific herring that had been held in captivity for...
Authors
Paul K. Hershberger, Jacob L. Gregg, A.C. Seitz, Brenda L. Norcross, J.C. Payne, A.N. Kagley, B Meloy

Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout–derived cell line, RTG-2 Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout–derived cell line, RTG-2

Infectious haematopoietic necrosis virus (IHNV) is one of the most important viral pathogens of salmonids. In rainbow trout, IHNV isolates in the M genogroup are highly pathogenic, while U genogroup isolates are significantly less pathogenic. We show here that, at a multiplicity of infection (MOI) of 1, a representative U type strain yielded 42‐fold less infectious virus than an M type...
Authors
Jeong Woo Park, Chang Hoon Moon, Andrew Wargo, Maureen K. Purcell, Gael Kurath
Was this page helpful?