These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in invertebrates, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation; radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Histopathology results are given for liver and kidney lesions generally associated with metals toxicoses in small rodents. Samples were collected at the Pinenut Mine (active mining) and Arizona 1 Mine (post-production) in the Grand Canyon watershed. The non-mineralized reference sites were Little Robinson Tank and Wild Band Reservoir (Valley pocket gophers only). Results for the pre-production site (Canyon Mine) were previously presented at https://doi.org/10.5066/F7QF8R16.