Skip to main content
U.S. flag

An official website of the United States government

Data for Assessing the Status of Macroinvertebrate Communities and Sediment Toxicity in the Buffalo River Area of Concern, New York

September 27, 2021

Data from 10-day sediment toxicity tests of bed sediments from the Buffalo River Area of Concern (AOC) and reference reaches on the Buffalo River upstream of the AOC, Erie County, New York, respectively. Specifically, the data was used to compare the survival and growth of two macroinvertebrate species in sediments from study sites and laboratory controls. Results are from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. Sediment samples were collected from 8 sites within the AOC and from 6 reference sites upstream of the AOC. Bed-sediments were collected from depositional areas using a petite Ponar (0.03 square meter) dredge. At each site, approximately five grabs were composited into a bucket, mixed, and a 4-liter (L) subsample was stored in a polyethylene container. Sediment toxicity testing were then conducted by a contract laboratory to quantify toxicity with the dipteran, Chironomus dilutus, and the amphipod, Hyalella azteca, during 10-day survival and growth bioassays following USEPA test methods 100.2 and 100.1, respectively. (U.S. Environmental Protection Agency, 2000, Methods for measuring the toxicity and bioaccumulation of sediment associated contaminants with freshwater invertebrates. Second edition: U.S. Environmental Protection Agency, Office of Research and Development EPA 600/R-99/064.) This spreadsheet contains 16 columns. The first 8 columns describe the sample collection information, the remaining 8 columns provide the survival and growth results of two test species used in sediment toxicity tests. The data include the stream name, site ID, National Water Information System (NWIS) ID, latitude and longitude, replicate number, and site type. The following eight columns present results from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. The following endpoints were measured for each species following the 10-day exposure: number of surviving organisms, percentage of organisms surviving (hereafter survival), average ash-free dry weight of the surviving organisms (hereafter growth), and ash-free dry weight of the surviving organisms divided by the initial number of organisms (hereafter biomass).