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A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation

August 7, 2018

Objective

We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.

Results

Of the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4–337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1–9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.

Citation Information

Publication Year 2018
Title A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
DOI 10.1186/s13104-018-3671-4
Authors Jason A. Ferrante, Michelle R. Giles, Emily Benzie, Margaret Hunter
Publication Type Article
Publication Subtype Journal Article
Series Title BMC Research Notes
Series Number
Index ID 70198561
Record Source USGS Publications Warehouse
USGS Organization Wetland and Aquatic Research Center