Background
Environmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be fully established, with one area being the calculation and interpretation of assay limit of detection (LOD) and limit of quantification (LOQ).
Aims
Here, we propose establishing consistent methods for determining and reporting of LOD and LOQ for single‐species quantitative PCR (qPCR) eDNA studies.
Materials & Methods/ Results
We utilize datasets from multiple cooperating laboratories to demonstrate both a discrete threshold approach and a curve‐fitting modeling approach for determining LODs and LOQs for eDNA qPCR assays. We also provide details of an R script developed and applied for the modeling method.
Discussion/Conclusions
Ultimately, standardization of how LOD and LOQ are determined, interpreted, and reported for eDNA assays will allow for more informed interpretation of assay results, more meaningful interlaboratory comparisons of experiments, and enhanced capacity for assessing the relative technical quality and performance of different eDNA qPCR assays.
Citation Information
| Publication Year | 2019 |
|---|---|
| Title | Reporting the limits of detection and quantification for environmental DNA assays |
| DOI | 10.1002/edn3.29 |
| Authors | Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret Hunter, Craig Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Environmental DNA |
| Index ID | 70205435 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Columbia Environmental Research Center; Upper Midwest Environmental Sciences Center; Wetland and Aquatic Research Center |
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Joel P Stokdyk
Biologist (LIDE, USGS Liaison)
Cathy Richter, PhD
Supervisory Research Molecular Biologist
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Joel P Stokdyk
Biologist (LIDE, USGS Liaison)EmailPhoneExt112Cathy Richter, PhD
Supervisory Research Molecular BiologistEmailPhone