Diane Waller
Science and Products
Carbon Dioxide for General Aquatic Invasive Species Control
Asian Carp Control: Carbon Dioxide
Carbon dioxide (CO2) is being evaluated as a new fishery chemical for general aquatic invasive species control. Most research has focused on its effectiveness as a behavioral...
Effects of Carbon Dioxide on Non-Target Organisms
Asian Carp Control: Carbon Dioxide
Chemical controls are a vital component of many effective Integrated Pest Management plans. Carbon dioxide (CO2) is being evaluated as a new fishery chemical for invasive Asian...
Transformation methods for the glochidia of the spectaclecase mussel Cumberlandia monodonta
The spectaclecase mussel, Cumberlandia monodonta, was effectively listed as federally endangered in April 2012 (https://federalregister.gov/a/2012-5603). It is endemic to the Mississippi, Ohio and Missouri River basins and historically inhabited 44 streams in these basins (USFWS 2014). Currently, the species is known to inhabit 20 of the...
Evaluation of lethal and sublethal responses of dreissenid and unionid mussels to elevated carbon dioxide
Control technology for dreissenid mussels currently relies heavily on chemical molluscicides that can be both costly and ecologically harmful. There is a need to develop more environmentally neutral control tools to manage dreissenid mussels. Carbon dioxide has shown toxicity to several species of invasive bivalves, including zebra mussels (Dreissena polymorpha) and the Asian clam (Corbicula...
Assessment of uncontained Zequanox applications for zebra mussel control in a Midwestern lake
Zebra mussels (Dreissena polymorpha) are invasive bivalves that have perturbed aquatic ecosystems within North America since their introduction in the mid-1980s. Control of zebra mussels has largely been restricted to raw water conveyance systems and associated infrastructures because few control products are registered for application in surface...
Luoma, James A.; Waller, Diane L.; Severson, Todd J.; Barbour, Matthew T.; Wise, Jeremy K.; Lord, Eric G.; Bartsch, Lynn A.; Bartsch, Michelle R.
Lethal and sublethal responses of native mussels (Unionidae: Lampsilis siliquoidea and Lampsilis higginsii) to elevated carbon dioxide
Levels of carbon dioxide (CO2) that have been proposed for aquatic invasive species control (24 000 – 96 000 μatm partial pressure of CO2 (PCO2); 1 atm = 101.325 kPa) were tested on two juvenile mussels, the fatmucket (Lampsilis siliquoidea) and the US federally endangered Higgins’ eye (Lampsilis higginsii). A suite of responses (survival,...
Waller, Diane L.; Bartsch, Michelle R.; Bartsch, Lynn A.; Jackson, Craig Building a response network to investigate potential pathogens associated with unionid mortality events
Unexplained mortality events have confounded the mussel conservation community for over 30 years. While the effects of chemical pollutants and environmental factors have been examined, few investigations have focused on the identification of potential mussel pathogens. Consequently, very little is known regarding the impact that pathogens have on...
Leis, Eric; Waller, Diane L.; Knowles, Susan; Goldberg, Tony; Putnam, Joel G.; Richard, Jordan; Erickson, Sarah; Blevins, Emilie; Weinzinger, Jesse Use of carbon dioxide in zebra mussel (Dreissena polymorpha) control and safety to a native freshwater mussel (Fatmucket, Lampsilis siliquoidea)
Control technology for dreissenid mussels (Dreissena polymorpha and D. bugensis) currently relies heavily on chemical molluscicides that can be both costly and ecologically harmful. There is a need for more environmentally neutral tools to manage dreissenid mussels, particularly in cooler water. Carbon dioxide (CO2) has been shown to be lethal to...
Waller, Diane L.; Bartsch, Michelle R. Effects of the biopesticide Zequanox® on reproduction and early development of the fathead minnow (Pimephales promelas)
The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems in the United States. Previous toxicity trials with nontarget organisms, including several young-of-the-year fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenid mussels, but data are lacking on the treatment-related...
Waller, Diane L.; Luoma, James A. Effects of carbon dioxide on juveniles of the freshwater mussel (Lampsilis siliquoidea [Unionidae])
Carbon dioxide (CO2) has shown promise as a tool to control movements of invasive Asian carp, but its effects on native freshwater biota have not been well studied. The authors evaluated lethal and sublethal responses of juvenile fatmucket (Lampsilis siliquoidea) mussels to CO2 at levels (43–269 mg/L, mean concentration) that bracket...
Waller, Diane L.; Bartsch, Michelle R.; Fredricks, Kim T.; Bartsch, Lynn A.; Schleis, Susan M.; Lee, Sheldon Safety of the molluscicide Zequanox (R) to nontarget macroinvertebrates Gammarus lacustris (Amphipoda: Gammaridae) and Hexagenia spp. (Ephemeroptera: Ephemeridae)
Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A), that was developed to control dreissenid mussels. In 2014, Zequanox became the second product registered by the United States Environmental Protection Agency (USEPA) for use in open water environments as a molluscicide. Previous nontarget...
Waller, Diane L.; Luoma, James A.; Erickson, Richard A. Effects of spray-dried Pseudomonas fluorescens, strain CL145A (Zequanox®) on reproduction and early development of the fathead minnow (Pimephales promelas).
The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems. Previous toxicity trials with nontarget organisms, including young-of-the year of several fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenids. However, data are lacking on its safety to reproductive and early life stages...
Waller, Diane L.; Luoma, James A.
Safety of spray-dried powder formulated Pseudomonas fluorescens strain CL145A exposure to subadult/adult unionid mussels during simulated open-water treatments
The exposure effects of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) on the survival of seven species of unionid mussels endemic to the Great Lakes and Mississippi River basins was evaluated in this study. The study exposures were completed within replicated 350-liter test tanks...
Luoma, James A.; Weber, Kerry L.; Waller, Diane L.; Wise, Jeremy K.; Mayer, Denise A.; Aloisi, Douglas B. Acute toxicity and accumulation of the piscicide 3-trifluoromethyl-4-nitrophenol (TFM) in freshwater mussels (Bivalvia: Unionidae)
We compared the acute toxicity and initial accumulation of the piscicide TFM (3-trifluoromethyl-4-nitrophenol) in the freshwater unionacean mussels, Obliquaria reflexa and Fusconaia flava. Acute 48 h toxicity tests were conducted to determine the LC50 values for each species. The initial uptake clearances of TFM were measured by exposing the...
Waller, Diane L.; Rach, Jeffrey J.; Luoma, James A. Evaluation of freshwater mussel relocation as a conservation and management strategy
The relocation of unionacean mussels is commonly used as a conservation and management tool in large rivers and streams. Relocation has been used to recolonize areas where mussel populations have been eliminated by prior pollution events, to remove mussels from construction zones and to re-establish populations of endangered species. More recently...
Cope, W. Gregory; Waller, Diane L.