Recent advancements in assessments using early life stages of test organisms (like the larval mussels shown) provide robust alternatives to full life-cycle chronic ecotoxicity tests. For more information on aquatic toxicology test organisms refer to the U.S.
Doug Hardesty
Doug is a Biological Science Lab Technician at the Columbia Environmental Research Center.
Education and Certifications
B.S. Fisheries and Wildlife, University of Missouri-Columbia, 1988
Science and Products
Filter Total Items: 18
Method development for a short-term 7-day toxicity test with unionid mussels
The US Environmental Protection Agency's short-term freshwater effluent test methods include a fish (Pimephales promelas), a cladoceran (Ceriodaphnia dubia), and a green alga (Raphidocelis subcapitata). There is a recognized need for additional taxa to accompany the three standard species for effluent testing. An appropriate additional taxon is unionid mussels because mussels are widely distribute
Authors
Ning Wang, James L. Kunz, Douglas K. Hardesty, Jeffery Steevens, Teresa J. Norberg-King, Edward J. Hammer, Candice R. Bauer, Tom Augspurger, Suzanne Dunn, David Martinez, M. Christopher Barnhart, Jordan Murray, Marcus Bowersox, John F. Roberts, Robert B. Bringolf, Robert Ratajczak, Serena Ciparis, W. Gregory Cope, Sean B. Buczek, Daniel Farrar, Lauren May, Mailee Garton, Patricia L. Gillis, James Bennett, Joseph Salerno, Brian Hester, Richard Lockwood, Christopher Tarr, Dennis McIntyre, Jonathan Wardell
Survival and growth of freshwater pulmonate and nonpulmonate snails in 28-day exposures to copper, ammonia, and pentachlorophenol
We performed toxicity tests with two species of pulmonate snails (Lymnaea stagnalis and Physa gyrina) and four taxa of nonpulmonate snails in the family Hydrobiidae (Pyrgulopsis robusta,Taylorconcha serpenticola, Fluminicola sp., and Fontigens aldrichi). Snails were maintained in static-renewal or recirculating culture systems with adults removed periodically to isolate cohorts of offspring for to
Authors
John M. Besser, Rebecca A. Dorman, Douglas K. Hardesty, Christopher G. Ingersoll
Acute and chronic toxicity of sodium sulfate to four freshwater organisms in water-only exposures
The acute and chronic toxicity of sulfate (tested as sodium sulfate) was determined in diluted well water (hardness of 100 mg/L and pH 8.2) with a cladoceran (Ceriodaphnia dubia; 2-d and 7-d exposures), a midge (Chironomus dilutus; 4-d and 41-d exposures), a unionid mussel (pink mucket, Lampsilis abrupta; 4-d and 28-d exposures), and a fish (fathead minnow, Pimephales promelas; 4-d and 34-d exposu
Authors
Ning Wang, Rebecca A. Consbrock, Christopher G. Ingersoll, Douglas K. Hardesty, William G. Brumbaugh, Edward J. Hammer, Candice R. Bauer, David R. Mount
Chronic sensitivity of white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) to cadmium, copper, lead, or zinc in laboratory water-only exposures
Chronic toxicity of cadmium, copper, lead, or zinc to white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) was evaluated in water-only exposures started with newly hatched larvae or approximately 1-mo-old juveniles. The 20% effect concentration (EC20) for cadmium from the sturgeon tests was higher than the EC20 from the trout tests, whereas the EC20 for copper, lead, or
Authors
Ning Wang, Christopher G. Ingersoll, Rebecca A. Dorman, William G. Brumbaugh, Christopher A. Mebane, James L. Kunz, Douglas K. Hardesty
Use of lethal short-term chlorine exposures to limit release of non-native freshwater organisms
Fish hatcheries and other types of aquatic facilities are potential sources for the introduction of nonnative species
of fish or aquatic invertebrates into watersheds. Chlorine has been suggested for use to kill organisms that might be
released from the effluent of a facility. While acute LC50s (concentrations lethal to 50% of organisms exposed for
up to 96 h) for chlorine are available for some s
Authors
Christopher G. Ingersoll, Eric L. Brunson, Douglas K. Hardesty, Jamie P. Hughes, Brittany L. King, Catherine T. Phillips
Contaminants in stream sediments from seven United States metropolitan areas: part II—sediment toxicity to the amphipod Hyalella azteca and the midge Chironomus dilutus
Relationships between sediment toxicity and sediment chemistry were evaluated for 98 samples collected from seven metropolitan study areas across the United States. Sediment-toxicity tests were conducted with the
amphipod Hyalella azteca (28 day exposures) and with the midge Chironomus dilutus (10 day exposures). Overall, 33 % of the samples were toxic to amphipods and 12 % of the samples were to
Authors
Nile E. Kemble, Douglas K. Hardesty, Christopher G. Ingersoll, James L. Kunz, Paul K. Sibley, Daniel L. Calhoun, Robert J. Gilliom, Kathryn Kuivila, Lisa H. Nowell, Patrick W. Moran
Toxicity of carbon nanotubes to freshwater aquatic invertebrates
Carbon nanotubes (CNTs) are hydrophobic in nature and thus tend to accumulate in sediments if released into aquatic environments. As part of our overall effort to examine the toxicity of carbon-based nanomaterials to sediment-dwelling invertebrates, we have evaluated the toxicity of different types of CNTs in 14-d water-only exposures to an amphipod (Hyalella azteca), a midge (Chironomus dilutus),
Authors
Joseph N. Mwangi, Ning Wang, Christopher G. Ingersoll, Doug K. Hardesty, Eric L. Brunson, Hao Li, Baolin Deng
An evaluation of the influence of substrate on the response of juvenile freshwater mussels (fatmucket, Lampsilis siliquoidea) in acute water exposures to ammonia
Acute 96-h ammonia toxicity to three-month-old juvenile mussels (Lampsilis siliquoidea) was evaluated in four treatments (water-only, water-only with feeding, water and soil, and water and sand) using an exposure unit designed to maintain consistent pH and ammonia concentrations in overlying water and in pore water surrounding the substrates. Median effect concentrations (EC50s) for total ammonia
Authors
J. Miao, M.C. Barnhart, Eric L. Brunson, Douglas K. Hardesty, Christopher G. Ingersoll, Ning Wang
Sensitivity of early life stages of freshwater mussels (Unionidae) to acute and chronic toxicity of lead, cadmium, and zinc in water
Toxicity of lead, cadmium, or zinc to early life stages of freshwater mussels (fatmucket, Lampsilis siliquoidea; Neosho mucket, L. rafinesqueana) was evaluated in 48‐h exposures with mussel larvae (glochidia), in 96‐h exposures with newly transformed (5‐d‐old) and two‐ or six‐month‐old juvenile mussels, or in 28‐d exposures with two‐ or four‐month‐old mussels in reconstituted soft water. The 24‐h
Authors
Ning Wang, Christopher G. Ingersoll, Chris D. Ivey, Douglas K. Hardesty, Thomas W. May, T. Augspurger, A.D. Roberts, E. Van Genderen, M.C. Barnhart
Acute toxicity of copper, ammonia, and chlorine to glochidia and juveniles of freshwater mussels (Unionidae)
The objective of the present study was to determine acute toxicity of copper, ammonia, or chlorine to larval (glochidia) and juvenile mussels using the recently published American Society for Testing and Materials (ASTM) Standard guide for conducting laboratory toxicity tests with freshwater mussels. Toxicity tests were conducted with glochidia (24- to 48-h exposures) and juveniles (96-h exposures
Authors
N. Wang, C.G. Ingersoll, D.K. Hardesty, C.D. Ivey, J.L. Kunz, T.W. May, F.J. Dwyer, A.D. Roberts, T. Augspurger, C.M. Kane, R. J. Neves, M.C. Barnhart
Chronic toxicity of copper and ammonia to juvenile freshwater mussels (Unionidae)
The objectives of the present study were to develop methods for conducting chronic toxicity tests with juvenile mussels under flow-through conditions and to determine the chronic toxicity of copper and ammonia to juvenile mussels using these methods. In two feeding tests, two-month-old fatmucket (Lampsilis siliquoidea) and rainbow mussel (Villosa iris) were fed various live algae or nonviable alga
Authors
N. Wang, C.G. Ingersoll, I.E. Greer, D.K. Hardesty, C.D. Ivey, J.L. Kunz, W. G. Brumbaugh, F.J. Dwyer, A.D. Roberts, T. Augspurger, C.M. Kane, R. J. Neves, M.C. Barnhart
Assessing contaminant sensitivity of endangered and threatened aquatic species: Part III. Effluent toxicity tests
Toxicity tests using standard effluent test procedures described by the U.S. Environmental Protection Agency were conducted with Ceriodaphnia dubia, fathead minnows (Pimephales promelas), and seven threatened and endangered (listed) fish species from four families: (1) Acipenseridae: shortnose sturgeon (Acipenser brevirostrum); (2) Catostomidae; razorback sucker (Xyrauchen texanus); (3) Cyprinidae
Authors
F.J. Dwyer, D.K. Hardesty, C.E. Henke, C.G. Ingersoll, D.W. Whites, T. Augspurger, T.J. Canfield, D.R. Mount, F.L. Mayer
Larval Mussels
Recent advancements in assessments using early life stages of test organisms (like the larval mussels shown) provide robust alternatives to full life-cycle chronic ecotoxicity tests. For more information on aquatic toxicology test organisms refer to the U.S.
Science and Products
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Filter Total Items: 18
Method development for a short-term 7-day toxicity test with unionid mussels
The US Environmental Protection Agency's short-term freshwater effluent test methods include a fish (Pimephales promelas), a cladoceran (Ceriodaphnia dubia), and a green alga (Raphidocelis subcapitata). There is a recognized need for additional taxa to accompany the three standard species for effluent testing. An appropriate additional taxon is unionid mussels because mussels are widely distributeAuthorsNing Wang, James L. Kunz, Douglas K. Hardesty, Jeffery Steevens, Teresa J. Norberg-King, Edward J. Hammer, Candice R. Bauer, Tom Augspurger, Suzanne Dunn, David Martinez, M. Christopher Barnhart, Jordan Murray, Marcus Bowersox, John F. Roberts, Robert B. Bringolf, Robert Ratajczak, Serena Ciparis, W. Gregory Cope, Sean B. Buczek, Daniel Farrar, Lauren May, Mailee Garton, Patricia L. Gillis, James Bennett, Joseph Salerno, Brian Hester, Richard Lockwood, Christopher Tarr, Dennis McIntyre, Jonathan WardellSurvival and growth of freshwater pulmonate and nonpulmonate snails in 28-day exposures to copper, ammonia, and pentachlorophenol
We performed toxicity tests with two species of pulmonate snails (Lymnaea stagnalis and Physa gyrina) and four taxa of nonpulmonate snails in the family Hydrobiidae (Pyrgulopsis robusta,Taylorconcha serpenticola, Fluminicola sp., and Fontigens aldrichi). Snails were maintained in static-renewal or recirculating culture systems with adults removed periodically to isolate cohorts of offspring for toAuthorsJohn M. Besser, Rebecca A. Dorman, Douglas K. Hardesty, Christopher G. IngersollAcute and chronic toxicity of sodium sulfate to four freshwater organisms in water-only exposures
The acute and chronic toxicity of sulfate (tested as sodium sulfate) was determined in diluted well water (hardness of 100 mg/L and pH 8.2) with a cladoceran (Ceriodaphnia dubia; 2-d and 7-d exposures), a midge (Chironomus dilutus; 4-d and 41-d exposures), a unionid mussel (pink mucket, Lampsilis abrupta; 4-d and 28-d exposures), and a fish (fathead minnow, Pimephales promelas; 4-d and 34-d exposuAuthorsNing Wang, Rebecca A. Consbrock, Christopher G. Ingersoll, Douglas K. Hardesty, William G. Brumbaugh, Edward J. Hammer, Candice R. Bauer, David R. MountChronic sensitivity of white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) to cadmium, copper, lead, or zinc in laboratory water-only exposures
Chronic toxicity of cadmium, copper, lead, or zinc to white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) was evaluated in water-only exposures started with newly hatched larvae or approximately 1-mo-old juveniles. The 20% effect concentration (EC20) for cadmium from the sturgeon tests was higher than the EC20 from the trout tests, whereas the EC20 for copper, lead, orAuthorsNing Wang, Christopher G. Ingersoll, Rebecca A. Dorman, William G. Brumbaugh, Christopher A. Mebane, James L. Kunz, Douglas K. HardestyUse of lethal short-term chlorine exposures to limit release of non-native freshwater organisms
Fish hatcheries and other types of aquatic facilities are potential sources for the introduction of nonnative species of fish or aquatic invertebrates into watersheds. Chlorine has been suggested for use to kill organisms that might be released from the effluent of a facility. While acute LC50s (concentrations lethal to 50% of organisms exposed for up to 96 h) for chlorine are available for some sAuthorsChristopher G. Ingersoll, Eric L. Brunson, Douglas K. Hardesty, Jamie P. Hughes, Brittany L. King, Catherine T. PhillipsContaminants in stream sediments from seven United States metropolitan areas: part II—sediment toxicity to the amphipod Hyalella azteca and the midge Chironomus dilutus
Relationships between sediment toxicity and sediment chemistry were evaluated for 98 samples collected from seven metropolitan study areas across the United States. Sediment-toxicity tests were conducted with the amphipod Hyalella azteca (28 day exposures) and with the midge Chironomus dilutus (10 day exposures). Overall, 33 % of the samples were toxic to amphipods and 12 % of the samples were toAuthorsNile E. Kemble, Douglas K. Hardesty, Christopher G. Ingersoll, James L. Kunz, Paul K. Sibley, Daniel L. Calhoun, Robert J. Gilliom, Kathryn Kuivila, Lisa H. Nowell, Patrick W. MoranToxicity of carbon nanotubes to freshwater aquatic invertebrates
Carbon nanotubes (CNTs) are hydrophobic in nature and thus tend to accumulate in sediments if released into aquatic environments. As part of our overall effort to examine the toxicity of carbon-based nanomaterials to sediment-dwelling invertebrates, we have evaluated the toxicity of different types of CNTs in 14-d water-only exposures to an amphipod (Hyalella azteca), a midge (Chironomus dilutus),AuthorsJoseph N. Mwangi, Ning Wang, Christopher G. Ingersoll, Doug K. Hardesty, Eric L. Brunson, Hao Li, Baolin DengAn evaluation of the influence of substrate on the response of juvenile freshwater mussels (fatmucket, Lampsilis siliquoidea) in acute water exposures to ammonia
Acute 96-h ammonia toxicity to three-month-old juvenile mussels (Lampsilis siliquoidea) was evaluated in four treatments (water-only, water-only with feeding, water and soil, and water and sand) using an exposure unit designed to maintain consistent pH and ammonia concentrations in overlying water and in pore water surrounding the substrates. Median effect concentrations (EC50s) for total ammoniaAuthorsJ. Miao, M.C. Barnhart, Eric L. Brunson, Douglas K. Hardesty, Christopher G. Ingersoll, Ning WangSensitivity of early life stages of freshwater mussels (Unionidae) to acute and chronic toxicity of lead, cadmium, and zinc in water
Toxicity of lead, cadmium, or zinc to early life stages of freshwater mussels (fatmucket, Lampsilis siliquoidea; Neosho mucket, L. rafinesqueana) was evaluated in 48‐h exposures with mussel larvae (glochidia), in 96‐h exposures with newly transformed (5‐d‐old) and two‐ or six‐month‐old juvenile mussels, or in 28‐d exposures with two‐ or four‐month‐old mussels in reconstituted soft water. The 24‐hAuthorsNing Wang, Christopher G. Ingersoll, Chris D. Ivey, Douglas K. Hardesty, Thomas W. May, T. Augspurger, A.D. Roberts, E. Van Genderen, M.C. BarnhartAcute toxicity of copper, ammonia, and chlorine to glochidia and juveniles of freshwater mussels (Unionidae)
The objective of the present study was to determine acute toxicity of copper, ammonia, or chlorine to larval (glochidia) and juvenile mussels using the recently published American Society for Testing and Materials (ASTM) Standard guide for conducting laboratory toxicity tests with freshwater mussels. Toxicity tests were conducted with glochidia (24- to 48-h exposures) and juveniles (96-h exposuresAuthorsN. Wang, C.G. Ingersoll, D.K. Hardesty, C.D. Ivey, J.L. Kunz, T.W. May, F.J. Dwyer, A.D. Roberts, T. Augspurger, C.M. Kane, R. J. Neves, M.C. BarnhartChronic toxicity of copper and ammonia to juvenile freshwater mussels (Unionidae)
The objectives of the present study were to develop methods for conducting chronic toxicity tests with juvenile mussels under flow-through conditions and to determine the chronic toxicity of copper and ammonia to juvenile mussels using these methods. In two feeding tests, two-month-old fatmucket (Lampsilis siliquoidea) and rainbow mussel (Villosa iris) were fed various live algae or nonviable algaAuthorsN. Wang, C.G. Ingersoll, I.E. Greer, D.K. Hardesty, C.D. Ivey, J.L. Kunz, W. G. Brumbaugh, F.J. Dwyer, A.D. Roberts, T. Augspurger, C.M. Kane, R. J. Neves, M.C. BarnhartAssessing contaminant sensitivity of endangered and threatened aquatic species: Part III. Effluent toxicity tests
Toxicity tests using standard effluent test procedures described by the U.S. Environmental Protection Agency were conducted with Ceriodaphnia dubia, fathead minnows (Pimephales promelas), and seven threatened and endangered (listed) fish species from four families: (1) Acipenseridae: shortnose sturgeon (Acipenser brevirostrum); (2) Catostomidae; razorback sucker (Xyrauchen texanus); (3) CyprinidaeAuthorsF.J. Dwyer, D.K. Hardesty, C.E. Henke, C.G. Ingersoll, D.W. Whites, T. Augspurger, T.J. Canfield, D.R. Mount, F.L. Mayer - Multimedia
Larval Mussels
Recent advancements in assessments using early life stages of test organisms (like the larval mussels shown) provide robust alternatives to full life-cycle chronic ecotoxicity tests. For more information on aquatic toxicology test organisms refer to the U.S.
Recent advancements in assessments using early life stages of test organisms (like the larval mussels shown) provide robust alternatives to full life-cycle chronic ecotoxicity tests. For more information on aquatic toxicology test organisms refer to the U.S.