Hon Ip (Former Employee)
Science and Products
Human Influenza Virus Infects Sea Otters
U.S. Geological Survey (USGS) scientists, in collaboration with the Centers for Disease Control and Prevention, have discovered evidence of the same influenza virus (H1N1) in sea otters living off the coast of Washington State that caused the 2009 "swine flu" pandemic in humans. During a sea otter health monitoring projectconducted in 2011, researchers discovered antibodies for the pandemic 2009...
Filter Total Items: 13
Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate
This data set provides biological information from of a captive study of transgenic mice that received either passive serum transfers from black-footed ferrets (BFFs) that were vaccinated against SARS-CoV-2 or BFFs that were unvaccinated, or a monoclonal antibody against SARS-CoV-2 or lastly a placebo. After these treatments mice were challenged with SARS-CoV-2. The data included here...
Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America” Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America”
In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, raccoons, and skunks, 261 (72%) of the animals...
Data set on reticuloendotheliosis in Hawaiian birds Data set on reticuloendotheliosis in Hawaiian birds
Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR...
Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2
We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and...
Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs
Rabbit hemorrhagic disease, a notifiable foreign animal disease in the USA, was reported for the first time in wild native North American rabbits and hares in April 2020 in the southwestern USA. Affected species included the Desert Cottontail (Sylvilagus audubonii), Mountain Cottontail (S. nutallii), Black-tailed Jackrabbit (Lepus californicus), and Antelope Jackrabbit (L. alleni)...
Avian Influenza Virus Test Results from Active Surveillance of North American Wild Birds Collected by Department of Interior from 2006-2011 Avian Influenza Virus Test Results from Active Surveillance of North American Wild Birds Collected by Department of Interior from 2006-2011
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and counter measure development...
Filter Total Items: 103
Successful detection of Delta and Omicron Variants of SARS-CoV-2 by veterinary diagnostic laboratory participants in an interlaboratory comparison exercise Successful detection of Delta and Omicron Variants of SARS-CoV-2 by veterinary diagnostic laboratory participants in an interlaboratory comparison exercise
Background Throughout the COVID-19 pandemic, veterinary diagnostic laboratories have tested diagnostic samples for SARS-CoV-2 both in animals and over 6 million human samples. An evaluation of the performance of those laboratories is needed using blinded test samples to ensure that laboratories report reliable data to the public. This interlaboratory comparison exercise (ILC3) builds on...
Authors
Kaiping Deng, Sarah M. Nemser, Kirstin Frost, Laura B. Goodman, Hon S. Ip, Mary Lea Killian, Jodie Ulaszek, Shannon Kiener, Matthew Kmet, Steffen Uhlig, Karina Hettwer, Bertrand Colson, Kapil Nichani, Anja Schlierf, Andriy Tkachenko, Megan R. Miller, Ravinder Reddy, Gregory H. Tyson
Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction...
Authors
Erik K. Hofmeister, Kathryn M. Griffin, Hon S. Ip
An evaluation of avian influenza virus whole-genome sequencing approaches using nanopore technology An evaluation of avian influenza virus whole-genome sequencing approaches using nanopore technology
As exemplified by the global response to the SARS-CoV-2 pandemic, whole-genome sequencing played an important role in monitoring the evolution of novel viral variants and provided guidance on potential antiviral treatments. The recent rapid and extensive introduction and spread of highly pathogenic avian influenza virus in Europe, North America, and elsewhere raises the need for...
Authors
Hon S. Ip, Sarah Uhm, Mary Lea Killian, Mia K. Torchetti
Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus is thought to have originated in wild bats from Asia, and as the resulting pandemic continues into its third year, concerns have been raised that the virus will expand its host range and infect North American wildlife species, including bats. Mexican free-tailed bats (Tadarida brasiliensis) live in large colonies in...
Authors
Jeffrey S. Hall, Erik K. Hofmeister, Hon S. Ip, Sean Nashold, Ariel Elizabeth Leon, Carly Marie Malave, Elizabeth Falendysz, Tonie E. Rocke, M. Carossino, U. Balasuriya, Susan Knowles
Immunogenicity, safety, and anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate in captive black-footed ferrets (Mustela nigripes) and their susceptibility to viral challenge Immunogenicity, safety, and anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate in captive black-footed ferrets (Mustela nigripes) and their susceptibility to viral challenge
A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum...
Authors
Ariel Elizabeth Leon, Della Garelle, Airn Hartwig, Elizabeth Falendysz, Hon S. Ip, Julia S. Lankton, Tyler Tretten, Terry Spraker, Richard Bowen, Tonie E. Rocke
Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary...
Authors
Kaiping Deng, Steffen Uhlig, Laura B. Goodman, Hon S. Ip, Mary Lea Killiam, Sarah Nemser, Jodie Ulaszek, Shannon Kiener, Matthew Kmet, Kirstin Frost, Karina Hettwer, Bertrand Colson, Kapil Nichani, Anja Schlierf, Andriy Tkachenko, Mothomang Mlalazi Oyinloye, Scott Andrew, Ravinder Reddy, Gregory H. Tyson
Science and Products
Human Influenza Virus Infects Sea Otters
U.S. Geological Survey (USGS) scientists, in collaboration with the Centers for Disease Control and Prevention, have discovered evidence of the same influenza virus (H1N1) in sea otters living off the coast of Washington State that caused the 2009 "swine flu" pandemic in humans. During a sea otter health monitoring projectconducted in 2011, researchers discovered antibodies for the pandemic 2009...
Filter Total Items: 13
Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate
This data set provides biological information from of a captive study of transgenic mice that received either passive serum transfers from black-footed ferrets (BFFs) that were vaccinated against SARS-CoV-2 or BFFs that were unvaccinated, or a monoclonal antibody against SARS-CoV-2 or lastly a placebo. After these treatments mice were challenged with SARS-CoV-2. The data included here...
Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America” Data from the article “An opportunistic survey reveals an unexpected coronavirus diversity hotspot in North America”
In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near affected mink farms and testing them for SARS-CoV-2 and non-SARS coronaviruses. Among the 365 animals sampled, including domestic cats, mink, rodents, raccoons, and skunks, 261 (72%) of the animals...
Data set on reticuloendotheliosis in Hawaiian birds Data set on reticuloendotheliosis in Hawaiian birds
Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR...
Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2
We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and...
Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs Data from pathology of Lagovirus europaeus GI.2/RHDV2/b (rabbit hemorrhagic disease virus 2) in native North American lagomorphs
Rabbit hemorrhagic disease, a notifiable foreign animal disease in the USA, was reported for the first time in wild native North American rabbits and hares in April 2020 in the southwestern USA. Affected species included the Desert Cottontail (Sylvilagus audubonii), Mountain Cottontail (S. nutallii), Black-tailed Jackrabbit (Lepus californicus), and Antelope Jackrabbit (L. alleni)...
Avian Influenza Virus Test Results from Active Surveillance of North American Wild Birds Collected by Department of Interior from 2006-2011 Avian Influenza Virus Test Results from Active Surveillance of North American Wild Birds Collected by Department of Interior from 2006-2011
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and counter measure development...
Filter Total Items: 103
Successful detection of Delta and Omicron Variants of SARS-CoV-2 by veterinary diagnostic laboratory participants in an interlaboratory comparison exercise Successful detection of Delta and Omicron Variants of SARS-CoV-2 by veterinary diagnostic laboratory participants in an interlaboratory comparison exercise
Background Throughout the COVID-19 pandemic, veterinary diagnostic laboratories have tested diagnostic samples for SARS-CoV-2 both in animals and over 6 million human samples. An evaluation of the performance of those laboratories is needed using blinded test samples to ensure that laboratories report reliable data to the public. This interlaboratory comparison exercise (ILC3) builds on...
Authors
Kaiping Deng, Sarah M. Nemser, Kirstin Frost, Laura B. Goodman, Hon S. Ip, Mary Lea Killian, Jodie Ulaszek, Shannon Kiener, Matthew Kmet, Steffen Uhlig, Karina Hettwer, Bertrand Colson, Kapil Nichani, Anja Schlierf, Andriy Tkachenko, Megan R. Miller, Ravinder Reddy, Gregory H. Tyson
Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction...
Authors
Erik K. Hofmeister, Kathryn M. Griffin, Hon S. Ip
An evaluation of avian influenza virus whole-genome sequencing approaches using nanopore technology An evaluation of avian influenza virus whole-genome sequencing approaches using nanopore technology
As exemplified by the global response to the SARS-CoV-2 pandemic, whole-genome sequencing played an important role in monitoring the evolution of novel viral variants and provided guidance on potential antiviral treatments. The recent rapid and extensive introduction and spread of highly pathogenic avian influenza virus in Europe, North America, and elsewhere raises the need for...
Authors
Hon S. Ip, Sarah Uhm, Mary Lea Killian, Mia K. Torchetti
Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2 Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus is thought to have originated in wild bats from Asia, and as the resulting pandemic continues into its third year, concerns have been raised that the virus will expand its host range and infect North American wildlife species, including bats. Mexican free-tailed bats (Tadarida brasiliensis) live in large colonies in...
Authors
Jeffrey S. Hall, Erik K. Hofmeister, Hon S. Ip, Sean Nashold, Ariel Elizabeth Leon, Carly Marie Malave, Elizabeth Falendysz, Tonie E. Rocke, M. Carossino, U. Balasuriya, Susan Knowles
Immunogenicity, safety, and anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate in captive black-footed ferrets (Mustela nigripes) and their susceptibility to viral challenge Immunogenicity, safety, and anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate in captive black-footed ferrets (Mustela nigripes) and their susceptibility to viral challenge
A preliminary vaccination trial against the emergent pathogen, SARS-CoV-2, was completed in captive black-footed ferrets (Mustela nigripes; BFF) to assess safety, immunogenicity, and anti-viral efficacy. Vaccination and boosting of 15 BFF with purified SARS-CoV-2 S1 subunit protein produced a nearly 150-fold increase in mean antibody titers compared to pre-vaccination titers. Serum...
Authors
Ariel Elizabeth Leon, Della Garelle, Airn Hartwig, Elizabeth Falendysz, Hon S. Ip, Julia S. Lankton, Tyler Tretten, Terry Spraker, Richard Bowen, Tonie E. Rocke
Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary...
Authors
Kaiping Deng, Steffen Uhlig, Laura B. Goodman, Hon S. Ip, Mary Lea Killiam, Sarah Nemser, Jodie Ulaszek, Shannon Kiener, Matthew Kmet, Kirstin Frost, Karina Hettwer, Bertrand Colson, Kapil Nichani, Anja Schlierf, Andriy Tkachenko, Mothomang Mlalazi Oyinloye, Scott Andrew, Ravinder Reddy, Gregory H. Tyson
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government