Science and Products
Screening toxic compounds using cellular assays
This study is designed to be a high-throughput alternative to traditional methods of using whole fish assays to screen compounds for toxicity. This approach involves an initial pre-screening of a chemical databank to select molecules possessing characteristics identified as predictive criteria for potential toxicity to the fathead minnow, followed by cytotoxicity screening of selected compounds...
Determination of the Acute Toxicity of Model-based Candidate Chemical Toxicants to Native and Nonnative Fish Species in Static Exposures
Invasive fishes are of considerable concern to aquatic resource managers. For example, the common carp (Cyprinus carpio), originally brought over from Eurasia sometime in the 1800s are now ubiquitous in U.S. waters. Although they have been around for over 100 years and have become part of U.S. culture, they are still highly detrimental to aquatic ecosystems and a method to eradicate and control of...
A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Cli
Freshwater mussels show elevated viral richness and intensity during a mortality event
Freshwater mussels (Unionida) are among the world’s most imperiled taxa, but the relationship between freshwater mussel mortality events and infectious disease is largely unstudied. We surveyed viromes of a widespread and abundant species (mucket, Actinonaias ligamentina; syn: Ortmanniana ligamentina) experiencing a mortality event of unknown etiology in the Huron River, Michigan, in 2019–2020 and
Quantitative method development to determine feed consumption using a dye
Although there are many methods to determine ingestion and absorption of aquafeeds, none exist that are simple, cost-effective, and quantitative and that can mark fish with a long-lasting, visible indicator. In addition to aquafeed development, selective baits are needed that can be used for aquatic invasive species removal efforts, including for Grass Carp Ctenopharyngodon idella. Bait incorporat
A novel gonadotropic microsporidian parasite (Microsporidium clinchi n. sp.) infecting a declining population of pheasantshell mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of their population decline are often enigmatic, with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in t
Mussel mass mortality and the microbiome: Evidence for shifts in the bacterial microbiome of a declining freshwater bivalve
Freshwater mussels (Unionida) are suffering mass mortality events worldwide, but the causes remain enigmatic. Here, we describe an analysis of bacterial loads, community structure, and inferred metabolic pathways in the hemolymph of pheasantshells (Actinonaias pectorosa) from the Clinch River, USA, during a multi-year mass mortality event. Bacterial loads were approximately 2 logs higher in moribu
Literature review for candidate chemical control agents for nonnative crayfish
Nonnative crayfish are an immediate and pervasive threat to aquatic environments and their biodiversity. Crayfish control can be achieved by physical methods, water chemistry modification, biological methods, biocidal application, and application of crayfish physiology modifiers. The purpose of this report is to identify suitable candidates for potential control of nonnative crayfish through a com
Mass mortality in freshwater mussels (Actinonaias pectorosa) in the Clinch River, USA, linked to a novel densovirus
Freshwater mussels (order Unionida) are among the world’s most biodiverse but imperiled taxa. Recent unionid mass mortality events around the world threaten ecosystem services such as water filtration, nutrient cycling, habitat stabilization, and food web enhancement, but causes have remained elusive. To examine potential infectious causes of these declines, we studied mussels in Clinch River, Vir
Building a response network to investigate potential pathogens associated with unionid mortality events
Unexplained mortality events have confounded the mussel conservation community for over 30 years. While the effects of chemical pollutants and environmental factors have been examined, few investigations have focused on the identification of potential mussel pathogens. Consequently, very little is known regarding the impact that pathogens have on freshwater mussel health, especially as they pertai
Using silver and bighead carp cell lines for the identification of a unique metabolite fingerprint from thiram-specific chemical exposure
Conservation biology often requires the control of invasive species. One method is the development and use of biocides. Identifying new chemicals as part of the biocide registration approval process can require screening millions of compounds. Traditionally, screening new chemicals has been done in vivo using test organisms. Using in vitro (e.g., cell lines) and in silico (e.g., computer models) m
Science and Products
- Science
Screening toxic compounds using cellular assays
This study is designed to be a high-throughput alternative to traditional methods of using whole fish assays to screen compounds for toxicity. This approach involves an initial pre-screening of a chemical databank to select molecules possessing characteristics identified as predictive criteria for potential toxicity to the fathead minnow, followed by cytotoxicity screening of selected compounds...Determination of the Acute Toxicity of Model-based Candidate Chemical Toxicants to Native and Nonnative Fish Species in Static Exposures
Invasive fishes are of considerable concern to aquatic resource managers. For example, the common carp (Cyprinus carpio), originally brought over from Eurasia sometime in the 1800s are now ubiquitous in U.S. waters. Although they have been around for over 100 years and have become part of U.S. culture, they are still highly detrimental to aquatic ecosystems and a method to eradicate and control of... - Data
A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Cli - Publications
Freshwater mussels show elevated viral richness and intensity during a mortality event
Freshwater mussels (Unionida) are among the world’s most imperiled taxa, but the relationship between freshwater mussel mortality events and infectious disease is largely unstudied. We surveyed viromes of a widespread and abundant species (mucket, Actinonaias ligamentina; syn: Ortmanniana ligamentina) experiencing a mortality event of unknown etiology in the Huron River, Michigan, in 2019–2020 andQuantitative method development to determine feed consumption using a dye
Although there are many methods to determine ingestion and absorption of aquafeeds, none exist that are simple, cost-effective, and quantitative and that can mark fish with a long-lasting, visible indicator. In addition to aquafeed development, selective baits are needed that can be used for aquatic invasive species removal efforts, including for Grass Carp Ctenopharyngodon idella. Bait incorporatA novel gonadotropic microsporidian parasite (Microsporidium clinchi n. sp.) infecting a declining population of pheasantshell mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of their population decline are often enigmatic, with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in tMussel mass mortality and the microbiome: Evidence for shifts in the bacterial microbiome of a declining freshwater bivalve
Freshwater mussels (Unionida) are suffering mass mortality events worldwide, but the causes remain enigmatic. Here, we describe an analysis of bacterial loads, community structure, and inferred metabolic pathways in the hemolymph of pheasantshells (Actinonaias pectorosa) from the Clinch River, USA, during a multi-year mass mortality event. Bacterial loads were approximately 2 logs higher in moribuLiterature review for candidate chemical control agents for nonnative crayfish
Nonnative crayfish are an immediate and pervasive threat to aquatic environments and their biodiversity. Crayfish control can be achieved by physical methods, water chemistry modification, biological methods, biocidal application, and application of crayfish physiology modifiers. The purpose of this report is to identify suitable candidates for potential control of nonnative crayfish through a comMass mortality in freshwater mussels (Actinonaias pectorosa) in the Clinch River, USA, linked to a novel densovirus
Freshwater mussels (order Unionida) are among the world’s most biodiverse but imperiled taxa. Recent unionid mass mortality events around the world threaten ecosystem services such as water filtration, nutrient cycling, habitat stabilization, and food web enhancement, but causes have remained elusive. To examine potential infectious causes of these declines, we studied mussels in Clinch River, VirBuilding a response network to investigate potential pathogens associated with unionid mortality events
Unexplained mortality events have confounded the mussel conservation community for over 30 years. While the effects of chemical pollutants and environmental factors have been examined, few investigations have focused on the identification of potential mussel pathogens. Consequently, very little is known regarding the impact that pathogens have on freshwater mussel health, especially as they pertaiUsing silver and bighead carp cell lines for the identification of a unique metabolite fingerprint from thiram-specific chemical exposure
Conservation biology often requires the control of invasive species. One method is the development and use of biocides. Identifying new chemicals as part of the biocide registration approval process can require screening millions of compounds. Traditionally, screening new chemicals has been done in vivo using test organisms. Using in vitro (e.g., cell lines) and in silico (e.g., computer models) m