Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
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Mycobacterial infection in Northern snakehead (Channa argus) from the Potomac River catchment
The Northern snakehead, Channa argus (Cantor), is a non-native predatory fish that has become established regionally in some temperate freshwater habitats within the United States. Over the past decade, Northern snakehead populations have developed within aquatic ecosystems throughout the eastern USA, including the Potomac River system within Virginia, Maryland and Washington, D.C. Since this spec
Authors
Christine L. Densmore, Luke R. Iwanowicz, Anne Henderson, Deborah D. Iwanowicz, J.S. Odenkirk
Characterization of a novel hepadnavirus in the white sucker (Catostomus commersonii) from the Great Lakes Region of the USA
The white sucker Catostomus commersonii is a freshwater teleost often utilized as a resident sentinel. Here, we sequenced the full genome of a hepatitis B-like virus that infects white suckers from the Great Lakes Region of the USA. Dideoxysequencing confirmed the white sucker hepatitis B virus (WSHBV) has a circular genome (3542 bp) with the prototypical codon organization of hepadnaviruses. Elec
Authors
Cassidy M. Hahn, Luke R. Iwanowicz, Robert S. Cornman, Carla M. Conway, James R. Winton, Vicki S. Blazer
Estuarine bed-sediment-quality data collected in New Jersey and New York after Hurricane Sandy, 2013
This report describes a reconnaissance study of estuarine bed-sediment quality conducted June–October 2013 in New Jersey and New York after Hurricane Sandy in October 2012 to assess the extent of contamination and the potential long-term human and ecological impacts of the storm. The study, funded through the Disaster Relief Appropriations Act of 2013 (PL 113-2), was conducted by the U.S. Geologic
Authors
Jeffrey M. Fischer, Patrick J. Phillips, Timothy J. Reilly, Michael J. Focazio, Keith A. Loftin, William Benzel, Daniel Jones, Kelly L. Smalling, Shawn C. Fisher, Irene J. Fisher, Luke R. Iwanowicz, Kristin M. Romanok, Darkus E. Jenkins, Luke Bowers, Adam Boehlke, William T. Foreman, Anna C. Deetz, Lisa G. Carper, Thomas E. Imbrigiotta, Justin E. Birdwell
Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)
Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures were made on
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
A landscape-based reconnaissance survey of estrogenic activity in streams of the upper Potomac, upper James,and Shenandoah Rivers, USA
Endocrine-disrupting compounds (EDCs) are becoming of increasing concern in waterways of the USA and worldwide. What remains poorly understood, however, is how prevalent these emerging contaminants are in the environment and what methods are best able to determine landscape sources of EDCs. We describe the development of a spatially structured sampling design and a reconnaissance survey of estroge
Authors
John A. Young, Luke R. Iwanowicz, Adam J. Sperry, Vicki Blazer
Reproductive health indicators of fishes from Pennsylvania watersheds: association with chemicals of emerging concern
Fishes were collected at 16 sites within the three major river drainages (Delaware, Susquehanna, and Ohio) of Pennsylvania. Three species were evaluated for biomarkers of estrogenic/antiandrogenic exposure, including plasma vitellogenin and testicular oocytes in male fishes. Smallmouth bass Micropterus dolomieu, white sucker Catostomus commersonii, and redhorse sucker Moxostoma species were collec
Authors
V. S. Blazer, D.D. Iwanowicz, H.L. Walsh, A.J. Sperry, L. R. Iwanowicz, D.A. Alvarez, R.A. Brightbill, G. Smith, W.T. Foreman, R. Manning
Comparative responses to endocrine disrupting compounds in early life stages of Atlantic salmon, Salmo salar
Atlantic salmon (Salmo salar) are endangered anadromous fish that may be exposed to feminizing endocrine disrupting compounds (EDCs) during early development, potentially altering physiological capacities, survival and fitness. To assess differential life stage sensitivity to common EDCs, we carried out short-term (four day) exposures using three doses each of 17α-ethinylestradiol (EE2), 17β-estra
Authors
Tara A. Duffy, Luke R. Iwanowicz, Stephen D. McCormick
Assessment of the fish tumor beneficial use impairment in brown bullhead (Ameiurus nebulosus) at selected Great Lakes Areas of Concern
A total of 878 adult Brown Bullhead were collected at 11 sites within the Lake Erie and Lake Ontario drainages from 2011 to 2013. The sites included seven Areas of Concern (AOC; 670 individuals), one delisted AOC (50 individuals) and three non-AOC sites (158 individuals) used as reference sites. These fish were used to assess the “fish tumor or other deformities” beneficial use impairment. Fish we
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan P. Braham, Cassidy M. Hahn, Heather L. Walsh, Adam J. Sperry
Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens
Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that channel ca
Authors
Luke R. Iwanowicz, James L. Stafford, Reynaldo Patiño, Eva Bengten, Norman W. Miller, Vicki Blazer
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune function
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse affects from e
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish reporter was s
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 13
Filter Total Items: 21
No Result Found
Filter Total Items: 100
Mycobacterial infection in Northern snakehead (Channa argus) from the Potomac River catchment
The Northern snakehead, Channa argus (Cantor), is a non-native predatory fish that has become established regionally in some temperate freshwater habitats within the United States. Over the past decade, Northern snakehead populations have developed within aquatic ecosystems throughout the eastern USA, including the Potomac River system within Virginia, Maryland and Washington, D.C. Since this spec
Authors
Christine L. Densmore, Luke R. Iwanowicz, Anne Henderson, Deborah D. Iwanowicz, J.S. Odenkirk
Characterization of a novel hepadnavirus in the white sucker (Catostomus commersonii) from the Great Lakes Region of the USA
The white sucker Catostomus commersonii is a freshwater teleost often utilized as a resident sentinel. Here, we sequenced the full genome of a hepatitis B-like virus that infects white suckers from the Great Lakes Region of the USA. Dideoxysequencing confirmed the white sucker hepatitis B virus (WSHBV) has a circular genome (3542 bp) with the prototypical codon organization of hepadnaviruses. Elec
Authors
Cassidy M. Hahn, Luke R. Iwanowicz, Robert S. Cornman, Carla M. Conway, James R. Winton, Vicki S. Blazer
Estuarine bed-sediment-quality data collected in New Jersey and New York after Hurricane Sandy, 2013
This report describes a reconnaissance study of estuarine bed-sediment quality conducted June–October 2013 in New Jersey and New York after Hurricane Sandy in October 2012 to assess the extent of contamination and the potential long-term human and ecological impacts of the storm. The study, funded through the Disaster Relief Appropriations Act of 2013 (PL 113-2), was conducted by the U.S. Geologic
Authors
Jeffrey M. Fischer, Patrick J. Phillips, Timothy J. Reilly, Michael J. Focazio, Keith A. Loftin, William Benzel, Daniel Jones, Kelly L. Smalling, Shawn C. Fisher, Irene J. Fisher, Luke R. Iwanowicz, Kristin M. Romanok, Darkus E. Jenkins, Luke Bowers, Adam Boehlke, William T. Foreman, Anna C. Deetz, Lisa G. Carper, Thomas E. Imbrigiotta, Justin E. Birdwell
Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)
Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures were made on
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
A landscape-based reconnaissance survey of estrogenic activity in streams of the upper Potomac, upper James,and Shenandoah Rivers, USA
Endocrine-disrupting compounds (EDCs) are becoming of increasing concern in waterways of the USA and worldwide. What remains poorly understood, however, is how prevalent these emerging contaminants are in the environment and what methods are best able to determine landscape sources of EDCs. We describe the development of a spatially structured sampling design and a reconnaissance survey of estroge
Authors
John A. Young, Luke R. Iwanowicz, Adam J. Sperry, Vicki Blazer
Reproductive health indicators of fishes from Pennsylvania watersheds: association with chemicals of emerging concern
Fishes were collected at 16 sites within the three major river drainages (Delaware, Susquehanna, and Ohio) of Pennsylvania. Three species were evaluated for biomarkers of estrogenic/antiandrogenic exposure, including plasma vitellogenin and testicular oocytes in male fishes. Smallmouth bass Micropterus dolomieu, white sucker Catostomus commersonii, and redhorse sucker Moxostoma species were collec
Authors
V. S. Blazer, D.D. Iwanowicz, H.L. Walsh, A.J. Sperry, L. R. Iwanowicz, D.A. Alvarez, R.A. Brightbill, G. Smith, W.T. Foreman, R. Manning
Comparative responses to endocrine disrupting compounds in early life stages of Atlantic salmon, Salmo salar
Atlantic salmon (Salmo salar) are endangered anadromous fish that may be exposed to feminizing endocrine disrupting compounds (EDCs) during early development, potentially altering physiological capacities, survival and fitness. To assess differential life stage sensitivity to common EDCs, we carried out short-term (four day) exposures using three doses each of 17α-ethinylestradiol (EE2), 17β-estra
Authors
Tara A. Duffy, Luke R. Iwanowicz, Stephen D. McCormick
Assessment of the fish tumor beneficial use impairment in brown bullhead (Ameiurus nebulosus) at selected Great Lakes Areas of Concern
A total of 878 adult Brown Bullhead were collected at 11 sites within the Lake Erie and Lake Ontario drainages from 2011 to 2013. The sites included seven Areas of Concern (AOC; 670 individuals), one delisted AOC (50 individuals) and three non-AOC sites (158 individuals) used as reference sites. These fish were used to assess the “fish tumor or other deformities” beneficial use impairment. Fish we
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan P. Braham, Cassidy M. Hahn, Heather L. Walsh, Adam J. Sperry
Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens
Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that channel ca
Authors
Luke R. Iwanowicz, James L. Stafford, Reynaldo Patiño, Eva Bengten, Norman W. Miller, Vicki Blazer
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune function
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse affects from e
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish reporter was s
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government