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Robert Gale, PhD (Former Employee)

Science and Products

Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More

Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure...
By
Columbia Environmental Research Center

Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture

Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
By
Columbia Environmental Research Center

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
By
Columbia Environmental Research Center, Western Fisheries Research Center, Fish Health Program (FHP)

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Acute toxicity and clotting times of anticoagulant rodenticides of four fish species
By
Columbia Environmental Research Center
Filter Total Items: 41

Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence

Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Kristin Leet, Catherine A. Richter, Rachel Claunch, Robert Gale, Donald E. Tillitt, Luke R. Iwanowicz
By
Ecosystems Mission Area, Columbia Environmental Research Center

Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples

Chemically activated luciferase expression (CALUX) cell bioassays are popular tools for assessing endocrine activity of chemicals such as certain environmental contaminants. Although activity equivalents can be obtained from CALUX analysis, directly comparing these equivalents to those obtained from analytical chemistry methods can be problematic because of the complexity of endocrine...
Authors
Jennifer C. Brennan, Abigail Henke, Robert Gale, Diane K. Nicks, Donald Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds

Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha
Authors
Jessica Kristin Leet, Cathy A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins
By
Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection

Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Kristin Leet, Justin Greer, Cathy A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John D. Hansen
By
Ecosystems Mission Area, Columbia Environmental Research Center, Eastern Ecological Science Center, Western Fisheries Research Center, Fish Health Program (FHP)

Factors affecting sampling strategies for design of an effects‐directed analysis for endocrine‐active chemicals Factors affecting sampling strategies for design of an effects‐directed analysis for endocrine‐active chemicals

Effects‐directed analysis (EDA) is an important tool for identifying unknown bioactive components in a complex mixture. Such an analysis of endocrine‐active chemicals (EACs) from water sources has promising regulatory implications but also unique logistical challenges. We propose a conceptual EDA (framework) based on a critical review of EDA literature and concentrations of common EACs...
Authors
Jennifer Brennan, Robert W. Gale, David A. Alvarez, Jason P. Berninger, Jessica Kristin Leet, Yan Li, Tyler Wagner, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Anticoagulant rodenticides (ARs) areused in rateradication efforts on island wildlife refuges. ARbait pellets can get into coralreefareasduring broadcasting and leadto exposure ofnon-target organisms, such as marine fishes. The objective of this study was to determine the sensitivity of representative saltwater fishes, Red-toothed triggerfish (Odonus niger) and Black triggerfish (Melichthys...
Authors
Rachelle Riegerix, Mike Tanner, Robert W. Gale, Donald E. Tillitt
By
Ecosystems Mission Area, Columbia Environmental Research Center

Science and Products

Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More

Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure...
By
Columbia Environmental Research Center

Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture

Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
By
Columbia Environmental Research Center

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
By
Columbia Environmental Research Center, Western Fisheries Research Center, Fish Health Program (FHP)

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Acute toxicity and clotting times of anticoagulant rodenticides of four fish species
By
Columbia Environmental Research Center
Filter Total Items: 41

Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence

Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Kristin Leet, Catherine A. Richter, Rachel Claunch, Robert Gale, Donald E. Tillitt, Luke R. Iwanowicz
By
Ecosystems Mission Area, Columbia Environmental Research Center

Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples

Chemically activated luciferase expression (CALUX) cell bioassays are popular tools for assessing endocrine activity of chemicals such as certain environmental contaminants. Although activity equivalents can be obtained from CALUX analysis, directly comparing these equivalents to those obtained from analytical chemistry methods can be problematic because of the complexity of endocrine...
Authors
Jennifer C. Brennan, Abigail Henke, Robert Gale, Diane K. Nicks, Donald Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds

Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha
Authors
Jessica Kristin Leet, Cathy A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins
By
Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection

Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Kristin Leet, Justin Greer, Cathy A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John D. Hansen
By
Ecosystems Mission Area, Columbia Environmental Research Center, Eastern Ecological Science Center, Western Fisheries Research Center, Fish Health Program (FHP)

Factors affecting sampling strategies for design of an effects‐directed analysis for endocrine‐active chemicals Factors affecting sampling strategies for design of an effects‐directed analysis for endocrine‐active chemicals

Effects‐directed analysis (EDA) is an important tool for identifying unknown bioactive components in a complex mixture. Such an analysis of endocrine‐active chemicals (EACs) from water sources has promising regulatory implications but also unique logistical challenges. We propose a conceptual EDA (framework) based on a critical review of EDA literature and concentrations of common EACs...
Authors
Jennifer Brennan, Robert W. Gale, David A. Alvarez, Jason P. Berninger, Jessica Kristin Leet, Yan Li, Tyler Wagner, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Anticoagulant rodenticides (ARs) areused in rateradication efforts on island wildlife refuges. ARbait pellets can get into coralreefareasduring broadcasting and leadto exposure ofnon-target organisms, such as marine fishes. The objective of this study was to determine the sensitivity of representative saltwater fishes, Red-toothed triggerfish (Odonus niger) and Black triggerfish (Melichthys...
Authors
Rachelle Riegerix, Mike Tanner, Robert W. Gale, Donald E. Tillitt
By
Ecosystems Mission Area, Columbia Environmental Research Center
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