Publications
USGS divers in the Elwha River
WFRC zebrafish laboratory
Processing a Lost River sucker
Below is a list of available WFRC peer reviewed and published science.
Filter Total Items: 2517
Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis
Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum...
Authors
D.M. Chase, D.G. Elliott, R.J. Pascho
Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs
Three short fragments of recombinant subunit Pasteurella multocida toxin (rsPMT) were constructed for evaluation as candidate vaccines against progressive atrophic rhinitis (PAR) of swine. PMT-specific antibody secreting cells and evidence of cellular immunity were detected in rsPMT-immunized pigs following authentic PMT challenge or homologous antigen booster. Piglets immunized with...
Authors
Chih-Ming Liao, Chienjin Huang, Shih-Ling Hsuan, Zeng-Weng Chen, Wei-Cheng Lee, Cheng-I Liu, James R. Winton, Maw-Sheng Chien
An examination of environmental factors associated with Myxobolus cerebralis infection of wild trout in Pennsylvania An examination of environmental factors associated with Myxobolus cerebralis infection of wild trout in Pennsylvania
Salmonid whirling disease, caused by the myxosporean parasite Myxobolus cerebralis, was first observed in the United States in 1956 in central Pennsylvania. The parasite was subsequently discovered at several culture facilities throughout the state, and widespread distribution of this parasite via the stocking of subclinically infected brook trout Salvelinus fontinalis, rainbow trout...
Authors
Adam J. Kaeser, Charlotte Rasmussen, William E. Sharpe
Interaction Assessment: A modeling tool for predicting population dynamics from field data Interaction Assessment: A modeling tool for predicting population dynamics from field data
Interaction Assessment (INTASS) is a field and analytic methodology for constructing population dynamics models. Because data collected in generating a model for one species comprise much of the information needed for other species, a small increase in effort can result in simultaneous expressions for the dynamics of multiple species. These expressions can be used to simulate whole...
Authors
John M. Emlen, Jeffrey J. Duda, Matt D. Kirchhoff, D. Carl Freeman
Behavior and passage of juvenile salmonids during evaluation of a new fish screen at Cowlitz Falls Dam, 2006 Behavior and passage of juvenile salmonids during evaluation of a new fish screen at Cowlitz Falls Dam, 2006
In spring of 2006, Tacoma Power installed a new fish screen at the Cowlitz Falls Project (CFP) with the purpose of improving the collection of anadromous juvenile fish for downstream transport. The new fish screen was placed between the upstream baffle panels and the fish gates and flume system that lead to the Cowlitz Falls Fish Facility. We initiated a radio telemetry evaluation of the...
Authors
T.J. Kock, M.A Kritter, T.L. Liedtke, D.W. Rondorf
Migration depths of juvenile Chinook salmon and steelhead relative to total dissolved gas supersaturation in a Columbia River reservoir Migration depths of juvenile Chinook salmon and steelhead relative to total dissolved gas supersaturation in a Columbia River reservoir
The in situ depths of juvenile salmonids Oncorhynchus spp. were studied to determine whether hydrostatic compensation was sufficient to protect them from gas bubble disease (GBD) during exposure to total dissolved gas (TDG) supersaturation from a regional program of spill at dams meant to improve salmonid passage survival. Yearling Chinook salmon O. tshawytscha and juvenile steelhead O...
Authors
J.W. Beeman, A.G. Maule
Myxobolus cerebralis in native cutthroat trout of the Yellowstone Lake ecosystem Myxobolus cerebralis in native cutthroat trout of the Yellowstone Lake ecosystem
The exotic parasite Myxobolus cerebralis was first detected in native adult Yellowstone cutthroat trout Oncorhynchus clarkii bouvierii from Yellowstone Lake in 1998, seriously threatening the ecological integrity of this pristine, naturally functioning ecosystem. We immediately began to assess the prevalence and spatial extent of M. cerebralis infection in Yellowstone cutthroat trout...
Authors
T.M. Koel, D.L. Mahony, K. L. Kinnan, C. Rasmussen, C. J. Hudson, S. Murcia, B.L. Kerans
Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype
Viral haemorrhagic septicaemia virus (VHSV) was isolated from muskellunge, Esox masquinongy (Mitchill), caught from the NW portion of Lake St Clair, Michigan, USA in 2003. Affected fish exhibited congestion of internal organs; the inner wall of the swim bladder was thickened and contained numerous budding, fluid-filled vesicles. A virus was isolated using fish cell lines inoculated with...
Authors
E. Elsayed, M. Faisal, M. Thomas, G. Whelan, W. Batts, J. Winton
Introduction of translation stop codons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity Introduction of translation stop codons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity
A highly efficacious DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was mutated to introduce two stop codons to prevent glycoprotein translation while maintaining the plasmid DNA integrity and RNA transcription ability. The mutated plasmid vaccine, denoted pIHNw-G2stop, when injected intramuscularly into fish at high doses, lacked detectable...
Authors
K.A. Garver, C. M. Conway, Gael Kurath
Passage behavior of radio-tagged subyearling Chinook salmon at Bonneville Dam, 2005 Passage behavior of radio-tagged subyearling Chinook salmon at Bonneville Dam, 2005
No abstract available
Authors
R.E. Reagan, M.J. Farley, S.D. Evans, L.S Wright, N.S. Adams, D.W. Rondorf
Survival estimates of migrant juvenile salmonids through The Dalles Dam using radio telemetry, 2005 Survival estimates of migrant juvenile salmonids through The Dalles Dam using radio telemetry, 2005
No abstract available
Authors
T. Counihan, A. Puls, C. Walker, G. Holmberg