Publications
USGS divers in the Elwha River
WFRC zebrafish laboratory
Processing a Lost River sucker
Below is a list of available WFRC peer reviewed and published science.
Filter Total Items: 2525
Ballast water research at the WFRC Ballast water research at the WFRC
Invasive aquatic species are considered to be one of the greatest threats to marine biodiversity, coastal economies, and even human health. Ballast water is a primary vector for these bioinvasions. One method of reducing risk of ballast water introductions is mid-ocean exchange of marine ballast water. However, it is widely recognized that this practice has many limitations, including...
Authors
Lyman Thorsteinson
Introduction of translation stop condons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity Introduction of translation stop condons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity
A highly efficacious DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was mutated to introduce two stop codons to prevent glycoprotein translation while maintaining the plasmid DNA integrity and RNA transcription ability. The mutated plasmid vaccine, denoted pIHNw-G2stop, when injected intramuscularly into fish at high doses, lacked detectable...
Authors
Kyle A. Garver, Carla M. Conway, Gael Kurath
Effects of hydropower operations on spawning habitat, rearing habitat, and stranding/entrapment mortality of Fall Chinook Salmon in the Hanford Reach of the Columbia River Effects of hydropower operations on spawning habitat, rearing habitat, and stranding/entrapment mortality of Fall Chinook Salmon in the Hanford Reach of the Columbia River
No abstract available.
Authors
Donald R. Anglin, Steven L. Haeseker, Joseph J. Skalicky, Howard Schaller, Kenneth F. Tiffan, James R. Hatten, Paul Hoffarth, John Nugent, David Benner, Marv Yoshinaka
Behavior of White Sturgeon near hydroprojects and fishways Behavior of White Sturgeon near hydroprojects and fishways
During March 2004 through November 2005, white sturgeon movements were monitored at The Dalles Dam to characterize their distribution and movements in the immediate vicinity of the dam and to determine timing and routes of passage. A combination of radio and acoustic telemetry technologies were used to detect tagged fish within fishways and at strategic locations along the dam, the...
Authors
M.J. Parsley, C.D. Wright, B. K. Van Der Leeuw, E.E. Kofoot, C. A. Perry, M.L. Moser
Survival estimates of migrant juvenile salmonids through Bonneville Dam using radio telemetry, 2005 Survival estimates of migrant juvenile salmonids through Bonneville Dam using radio telemetry, 2005
During 2005, we evaluated the survival of radio-tagged yearling and subyearling Chinook salmon and steelhead trout through the Bonneville Dam spillway, powerhouses 1 and 2, the corner collector and juvenile bypass system (JBS) at powerhouse 2, and through all routes collectively using the route-specific survival model. Radio-tagged fish were released at The Dalles Dam and in the tailrace...
Authors
Timothy D. Counihan, Jill M. Hardiman, Chris Walker, Amy Puls, Glen Holmberg
Conservation of Toll-like receptor signaling pathways in teleost fish Conservation of Toll-like receptor signaling pathways in teleost fish
In mammals, toll-like receptors (TLR) recognize ligands, including pathogen-associated molecular patterns (PAMPs), and respond with ligand-specific induction of genes. In this study, we establish evolutionary conservation in teleost fish of key components of the TLR-signaling pathway that act as switches for differential gene induction, including MYD88, TIRAP, TRIF, TRAF6, IRF3, and IRF7...
Authors
M. K. Purcell, K.D. Smith, A. Aderem, L. Hood, J. R. Winton, J.C. Roach
White sturgeon mitigation and restoration in the Columbia and Snake rivers upstream from Bonneville Dam, Annual Progress Report April 2004 - March 2005. Report C White sturgeon mitigation and restoration in the Columbia and Snake rivers upstream from Bonneville Dam, Annual Progress Report April 2004 - March 2005. Report C
River discharge and water temperatures that occurred during April through July 2004 provided conditions suitable for spawning by white sturgeon downstream from Bonneville, The Dalles, John Day, and McNary dams. Optimal spawning temperatures in the four tailraces occurred for 3-4 weeks and coincided with the peak of the river hydrograph. However, the peak of the hydrograph was relatively...
Authors
M.J. Parsley, P. Kofoot
Effects of summer flow augmentation on the migratory behavior and survival of juvenile Snake River fall Chinook salmon. Annual report 2005 Effects of summer flow augmentation on the migratory behavior and survival of juvenile Snake River fall Chinook salmon. Annual report 2005
This report summarizes results of research activities conducted in 2004 and years previous to aid in the management and recovery of fall Chinook salmon Oncorhynchus tshawytscha in the Columbia River basin. For detailed summaries, we refer the reader to the abstracts given on the second page of each chapter. The Annual Reporting section includes information provided to fishery managers in...
Authors
Kenneth F. Tiffan, William P. Connor
Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, Infectious hematopoietic necrosis virus Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, Infectious hematopoietic necrosis virus
The fish rhabdovirus, Infectious hematopoietic necrosis virus (IHNV), is an important pathogen of salmonids. Cell culture assays have traditionally been used to quantify levels of IHNV in samples; however, real-time or quantitative RT-PCR assays have been proposed as a rapid alternative. For viruses having a single-stranded, negative-sense RNA genome, standard qRT-PCR assays do not...
Authors
Maureen K. Purcell, S. Alexandra Hart, Gael Kurath, James R. Winton
Potential use of offshore marine structures in rebuilding an overfished rockfish species, bocaccio (Sebastes paucispinis) Potential use of offshore marine structures in rebuilding an overfished rockfish species, bocaccio (Sebastes paucispinis)
Although bocaccio (Sebastes paucispinis) was an economically important rockfish species along the west coast of North America, overfishing has reduced the stock to about 7.4% of its former unfished population. In 2003, using a manned research submersible, we conducted fish surveys around eight oil and gas platforms off southern California as part of an assessment of the potential value...
Authors
M.S. Love, D.M. Schroeder, W. Lenarz, A. MacCall, A.S. Bull, L. Thorsteinson
Costimulatory receptors in a teleost fish: Typical CD28, elusive CTLA4 Costimulatory receptors in a teleost fish: Typical CD28, elusive CTLA4
T cell activation requires both specific recognition of the peptide-MHC complex by the TCR and additional signals delivered by costimulatory receptors. We have identified rainbow trout sequences similar to CD28 (rbtCD28) and CTLA4 (rbtCTLA4). rbtCD28 and rbtCTLA4 are composed of an extracellular Ig-superfamily V domain, a transmembrane region, and a cytoplasmic tail. The presence of a...
Authors
D. Bernard, B. Riteau, J.D. Hansen, R.B. Phillips, F. Michel, P. Boudinot, A. Benmansour
Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis
Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum...
Authors
D.M. Chase, D.G. Elliott, R.J. Pascho