This data release contains phytoplankton classification and enumeration results from two moving boat surveys conducted on July 15 - 18, 2019 and August 19 - 22, 2019. These data were collected from the Caloosahatchee River and estuary, the St. Lucie River and estuary, and Lake Okeechobee with funding provided by the USGS Priority Ecosystems Greater Everglades Program.