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W. Bane Schill (Former Employee)

Science and Products

Water samples thawing in preparation for eDNA analysis

Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Early detection systems for aquatic nuisance species have been improved by the advancement of polymerase chain reactions that amplify DNA sequences for decoding.
By
Eastern Ecological Science Center
Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Early detection systems for aquatic nuisance species have been improved by the advancement of polymerase chain reactions that amplify DNA sequences for decoding.
Learn More
Comparing eDNA sampling methods

Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Dwarf wedgemussel is a federally endangered freshwater mussel with a historic range spanning the Atlantic coast. However, populations have dramatically declines over the last 30 years. Therefore, knowledge of current population distribution and abundance is critical to resource managers in order to monitor the species over time and to guide recovery actions. Manual survey efforts (snorkel or scuba...
By
Ecosystems Mission Area, Eastern Ecological Science Center
Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Dwarf wedgemussel is a federally endangered freshwater mussel with a historic range spanning the Atlantic coast. However, populations have dramatically declines over the last 30 years. Therefore, knowledge of current population distribution and abundance is critical to resource managers in order to monitor the species over time and to guide recovery actions. Manual survey efforts (snorkel or scuba...
Learn More
Multispectral sensors

Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring
By
Eastern Ecological Science Center
Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring
Learn More
Genetics technician running a next generation sequencer

Molecular tools and methodologies

Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
By
Eastern Ecological Science Center
Molecular tools and methodologies

Molecular tools and methodologies

Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
Learn More

Cyanobacteria, cyanotoxins and associated characteristics across the North Atlantic Appalachian Region Cyanobacteria, cyanotoxins and associated characteristics across the North Atlantic Appalachian Region

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers...
By
New York Water Science Center

SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021

Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
By
Eastern Ecological Science Center

Matrix inhibition PCR and Microtox 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Matrix inhibition PCR and Microtox 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015

Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geological Survey has developed the...
By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.
Day 1 Sample Processing - Logging Samples
Day 1 Sample Processing - Logging Samples
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing - Logging Samples

Day 1 Sample Processing - Logging Samples

Deb Iwanowicz and Christine Densmore Logging in first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing - Logging Samples

Day 1 Sample Processing - Logging Samples

Deb Iwanowicz and Christine Densmore Logging in first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.
Day 1 Sample Processing
Day 1 Sample Processing
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing

Day 1 Sample Processing

Deb Iwanowicz and Christine Densmore processing first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center, Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing

Day 1 Sample Processing

Deb Iwanowicz and Christine Densmore processing first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center, Eastern Ecological Science Center
Filter Total Items: 24

Exploring the stony coral tissue loss disease bacterial pathobiome Exploring the stony coral tissue loss disease bacterial pathobiome

A devastating novel coral disease outbreak, referred to as Stony Coral Tissue Loss Disease (SCTLD), was first described in 2014. It is thought to have originated offshore of Miami-Dade County, FL, but has persisted and spread, affecting new reefs along the Florida Reef Tract and reefs of at least 8 other Caribbean jurisdictions. We investigated the microbial communities of clinically...
Authors
Deborah Iwanowicz, W. Bane Schill, C.M. Woodley, Andy Bruckner, K. Neely, Kay M. Briggs
By
Eastern Ecological Science Center

A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies

Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria...
Authors
Stephanie M. Rosales, Lindsay K. Huebner, James S. Evans, Amy Apprill, Andew C. Baker, Anthony J. Bellantuono, Marilyn E. Brandt, Abigail S. Clark, Javier del Campo, Caroline E. Dennison, Katherine R. Eaton, Naomi E. Huntley, Christina A. Kellogg, Monica Medina, Julie L. Meyer, Erinn M. Muller, Mauricio Rodriguez-Lanetty, Jennifer L. Salerno, W. Bane Schill, Erin N. Shilling, Julia Marie Stewart, Joshua D. Voss
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Eastern Ecological Science Center, St. Petersburg Coastal and Marine Science Center

Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus) Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus)

Indigenous freshwater mussels (Unionidae) are integral to riverine ecosystems, playing a pivotal role in aquatic food webs and providing ecological services. With populations on the decline worldwide, freshwater mussels are of conservation concern. In this study, we explore the propensity of the invasive Round Goby (Neogobius melanostomus) fish to prey upon indigenous freshwater mussels...
Authors
Kyle Clark, Deborah D. Iwanowicz, Luke R. Iwanowicz, Sara Mueller, Joshua Wisor, Casey Bradshaw-Wilson, W. Bane Schill, Jay R. Stauffer, Elizabeth W. Boyer
By
Eastern Ecological Science Center

Rapid implementation of high-frequency wastewater surveillance of SARS-CoV-2 Rapid implementation of high-frequency wastewater surveillance of SARS-CoV-2

There have been over 507 million cases of COVID-19, the disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulting in 6 million deaths globally. Wastewater surveillance has emerged as a valuable tool in understanding SARS-CoV-2 burden in communities. The National Wastewater Surveillance System (NWSS) partnered with the United States Geological Survey...
Authors
Meghan M. Holst, John Person, Wiley Jennings, Rory M. Welsh, Michael J. Focazio, Paul M. Bradley, W. Bane Schill, Amy E. Kirby, Zachary A. Marsh
By
Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, South Atlantic Water Science Center (SAWSC)

Novel microbiome dominated by Arcobacter during anoxic excurrent flow from an ocean blue hole in Andros Island, The Bahamas Novel microbiome dominated by Arcobacter during anoxic excurrent flow from an ocean blue hole in Andros Island, The Bahamas

Andros Island, The Bahamas, composed of porous carbonate rock, has about 175 inland blue holes and over 50 known submerged ocean caves along its eastern barrier reef. These ocean blue holes can have both vertical and horizontal zones that penetrate under the island. Tidal forces drive water flow in and out of these caves. King Kong Cavern has a vertical collapse zone and a deep...
Authors
Deborah D. Iwanowicz, Robert B Jonas, William B. Schill, Kay Marano-Briggs
By
Ecosystems Mission Area, Eastern Ecological Science Center

Capture of environmental DNA (eDNA) from water samples by flocculation Capture of environmental DNA (eDNA) from water samples by flocculation

The analysis of environmental DNA (eDNA) has become a widely used approach to problem solving in species management. The detection of cryptic species including invasive and (or) species at risk is the goal, typically accomplished by testing water and sediment for the presence of characteristic DNA signatures. Reliable and efficient procedures for the capture of eDNA are required...
Authors
W. Bane Schill
By
Ecosystems Mission Area, Eastern Ecological Science Center

Molecular identification of fecal contamination in the Elks Run Watershed, Jefferson County, West Virginia, 2016–17 Molecular identification of fecal contamination in the Elks Run Watershed, Jefferson County, West Virginia, 2016–17

The U.S. Geological Survey conducted a study using modern methods of molecular analysis aimed at attempting to identify the source(s) of fecal contamination that had been identified in previous studies conducted by the West Virginia Conservation Agency in the Elk Run watershed, Jefferson County, West Virginia. Water samples from multiple sites showing elevated fecal coliform counts were...
Authors
W. Bane Schill, Deborah D. Iwanowicz
By
Ecosystems Mission Area, Eastern Ecological Science Center

Detecting the undetectable: Characterization, optimization, and validation of an eDNA detection assay for the federally endangered dwarf wedgemussel, Alasmidonta heterodon (Bivalvia: Unionoida) Detecting the undetectable: Characterization, optimization, and validation of an eDNA detection assay for the federally endangered dwarf wedgemussel, Alasmidonta heterodon (Bivalvia: Unionoida)

Environmental (e)DNA assays are valuable tools for monitoring presence and distribution of cryptic species. Like many freshwater mussels, the dwarf wedgemussel, Alasmidonta heterodon numbers have dwindled and its range has diminished. As of its listing in 1993, only 10 to 20 locations were known to persist of the 70 Atlantic slope locations known historically. A qPCR assay to detect the...
Authors
W. Bane Schill, Heather S. Galbraith
By
Ecosystems Mission Area, Eastern Ecological Science Center

Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus) Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus)

Understanding the diet of invasive species helps researchers to more accurately assess the health, survivorship, growth, and stability of an invasive fish species, as well as their effects on native populations. Techniques capable of identifying multiple prey species from fish stomach contents have been developed. In this study, a multi-locus metabarcoding approach was used to identify...
Authors
Deborah D. Iwanowicz, W. Bane Schill, Lakyn R. Sanders, Tim Groves, Mary C. Groves
By
Ecosystems Mission Area, Eastern Ecological Science Center, Chesapeake Bay Activities

The complete maternal mitochondrial genome sequences of two imperiled North American freshwater mussels: Alasmidonta heterodon and Alasmidonta varicosa (Bivalvia: Unionoida: Unionidae) The complete maternal mitochondrial genome sequences of two imperiled North American freshwater mussels: Alasmidonta heterodon and Alasmidonta varicosa (Bivalvia: Unionoida: Unionidae)

The freshwater mussels Alasmidonta heterodon and A. varicosa historically inhabited rivers along the North American Atlantic coast from the Carolinas, U.S.A., to New Brunswick, CA. However, many populations have been extirpated, and A. heterodon is now federally listed in the U.S.A. as endangered, and both A. heterodon and A. varicosa are listed as vulnerable on the IUCN Red List. To...
Authors
Aaron Aunins, Cheryl L. Morrison, Heather Galbraith, Michael S. Eackles, W. Bane Schill, Tim L. King
By
Ecosystems Mission Area, Eastern Ecological Science Center

Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey

Blue mussels, Mytilus edulis, Linnaeus 1758 from southern Barnegat Bay, New Jersey were examined to determine the make-up of the normal blue mussel microbiome. Sequencing of 16S ribosomal DNA amplicons from gill and intestinal content microbiomes using the Illumina® MiSeq platform yielded 1,276,161 paired end sequence reads from the gill libraries and 1,092,333 paired end sequence reads...
Authors
William B. Schill, Deborah D. Iwanowicz, Cynthia R. Adams
By
Ecosystems Mission Area, Eastern Ecological Science Center

Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has...
Authors
Deborah D. Iwanowicz, William B. Schill, Deanna H. Olson, M. J. Adams, Christine L. Densmore, Robert S. Cornman, Cynthia R. Adams, Chester Figiel, Chauncey W. Anderson, Andrew R Blaustein, Tara Chestnut
By
Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, Forest and Rangeland Ecosystem Science Center, Oregon Water Science Center

Science and Products

Water samples thawing in preparation for eDNA analysis

Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Early detection systems for aquatic nuisance species have been improved by the advancement of polymerase chain reactions that amplify DNA sequences for decoding.
By
Eastern Ecological Science Center
Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Novel approach using flocculation to concentrate edna to search for early invasions of specific aquatic invasive species

Early detection systems for aquatic nuisance species have been improved by the advancement of polymerase chain reactions that amplify DNA sequences for decoding.
Learn More
Comparing eDNA sampling methods

Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Dwarf wedgemussel is a federally endangered freshwater mussel with a historic range spanning the Atlantic coast. However, populations have dramatically declines over the last 30 years. Therefore, knowledge of current population distribution and abundance is critical to resource managers in order to monitor the species over time and to guide recovery actions. Manual survey efforts (snorkel or scuba...
By
Ecosystems Mission Area, Eastern Ecological Science Center
Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Developing and testing eDNA markers for the Federally endangered dwarf wedgemussel, Alasmidonta heterodon and other key freshwater mussel species

Dwarf wedgemussel is a federally endangered freshwater mussel with a historic range spanning the Atlantic coast. However, populations have dramatically declines over the last 30 years. Therefore, knowledge of current population distribution and abundance is critical to resource managers in order to monitor the species over time and to guide recovery actions. Manual survey efforts (snorkel or scuba...
Learn More
Multispectral sensors

Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring
By
Eastern Ecological Science Center
Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring

Development of Deployable Multispectral Sensors for Monitoring
Learn More
Genetics technician running a next generation sequencer

Molecular tools and methodologies

Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
By
Eastern Ecological Science Center
Molecular tools and methodologies

Molecular tools and methodologies

Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
Learn More

Cyanobacteria, cyanotoxins and associated characteristics across the North Atlantic Appalachian Region Cyanobacteria, cyanotoxins and associated characteristics across the North Atlantic Appalachian Region

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers...
By
New York Water Science Center

SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021

Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
By
Eastern Ecological Science Center

Matrix inhibition PCR and Microtox 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Matrix inhibition PCR and Microtox 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015

Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geological Survey has developed the...
By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.
Day 1 Sample Processing - Logging Samples
Day 1 Sample Processing - Logging Samples
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing - Logging Samples

Day 1 Sample Processing - Logging Samples

Deb Iwanowicz and Christine Densmore Logging in first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing - Logging Samples

Day 1 Sample Processing - Logging Samples

Deb Iwanowicz and Christine Densmore Logging in first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.
Day 1 Sample Processing
Day 1 Sample Processing
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing

Day 1 Sample Processing

Deb Iwanowicz and Christine Densmore processing first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center, Eastern Ecological Science Center
Two women in full protective gear work on samples in a lab.

Day 1 Sample Processing

Day 1 Sample Processing

Deb Iwanowicz and Christine Densmore processing first wastewater samples in BSL3 Lab.

By
Eastern Ecological Science Center, Eastern Ecological Science Center
Filter Total Items: 24

Exploring the stony coral tissue loss disease bacterial pathobiome Exploring the stony coral tissue loss disease bacterial pathobiome

A devastating novel coral disease outbreak, referred to as Stony Coral Tissue Loss Disease (SCTLD), was first described in 2014. It is thought to have originated offshore of Miami-Dade County, FL, but has persisted and spread, affecting new reefs along the Florida Reef Tract and reefs of at least 8 other Caribbean jurisdictions. We investigated the microbial communities of clinically...
Authors
Deborah Iwanowicz, W. Bane Schill, C.M. Woodley, Andy Bruckner, K. Neely, Kay M. Briggs
By
Eastern Ecological Science Center

A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies

Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria...
Authors
Stephanie M. Rosales, Lindsay K. Huebner, James S. Evans, Amy Apprill, Andew C. Baker, Anthony J. Bellantuono, Marilyn E. Brandt, Abigail S. Clark, Javier del Campo, Caroline E. Dennison, Katherine R. Eaton, Naomi E. Huntley, Christina A. Kellogg, Monica Medina, Julie L. Meyer, Erinn M. Muller, Mauricio Rodriguez-Lanetty, Jennifer L. Salerno, W. Bane Schill, Erin N. Shilling, Julia Marie Stewart, Joshua D. Voss
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Eastern Ecological Science Center, St. Petersburg Coastal and Marine Science Center

Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus) Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus)

Indigenous freshwater mussels (Unionidae) are integral to riverine ecosystems, playing a pivotal role in aquatic food webs and providing ecological services. With populations on the decline worldwide, freshwater mussels are of conservation concern. In this study, we explore the propensity of the invasive Round Goby (Neogobius melanostomus) fish to prey upon indigenous freshwater mussels...
Authors
Kyle Clark, Deborah D. Iwanowicz, Luke R. Iwanowicz, Sara Mueller, Joshua Wisor, Casey Bradshaw-Wilson, W. Bane Schill, Jay R. Stauffer, Elizabeth W. Boyer
By
Eastern Ecological Science Center

Rapid implementation of high-frequency wastewater surveillance of SARS-CoV-2 Rapid implementation of high-frequency wastewater surveillance of SARS-CoV-2

There have been over 507 million cases of COVID-19, the disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulting in 6 million deaths globally. Wastewater surveillance has emerged as a valuable tool in understanding SARS-CoV-2 burden in communities. The National Wastewater Surveillance System (NWSS) partnered with the United States Geological Survey...
Authors
Meghan M. Holst, John Person, Wiley Jennings, Rory M. Welsh, Michael J. Focazio, Paul M. Bradley, W. Bane Schill, Amy E. Kirby, Zachary A. Marsh
By
Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, South Atlantic Water Science Center (SAWSC)

Novel microbiome dominated by Arcobacter during anoxic excurrent flow from an ocean blue hole in Andros Island, The Bahamas Novel microbiome dominated by Arcobacter during anoxic excurrent flow from an ocean blue hole in Andros Island, The Bahamas

Andros Island, The Bahamas, composed of porous carbonate rock, has about 175 inland blue holes and over 50 known submerged ocean caves along its eastern barrier reef. These ocean blue holes can have both vertical and horizontal zones that penetrate under the island. Tidal forces drive water flow in and out of these caves. King Kong Cavern has a vertical collapse zone and a deep...
Authors
Deborah D. Iwanowicz, Robert B Jonas, William B. Schill, Kay Marano-Briggs
By
Ecosystems Mission Area, Eastern Ecological Science Center

Capture of environmental DNA (eDNA) from water samples by flocculation Capture of environmental DNA (eDNA) from water samples by flocculation

The analysis of environmental DNA (eDNA) has become a widely used approach to problem solving in species management. The detection of cryptic species including invasive and (or) species at risk is the goal, typically accomplished by testing water and sediment for the presence of characteristic DNA signatures. Reliable and efficient procedures for the capture of eDNA are required...
Authors
W. Bane Schill
By
Ecosystems Mission Area, Eastern Ecological Science Center

Molecular identification of fecal contamination in the Elks Run Watershed, Jefferson County, West Virginia, 2016–17 Molecular identification of fecal contamination in the Elks Run Watershed, Jefferson County, West Virginia, 2016–17

The U.S. Geological Survey conducted a study using modern methods of molecular analysis aimed at attempting to identify the source(s) of fecal contamination that had been identified in previous studies conducted by the West Virginia Conservation Agency in the Elk Run watershed, Jefferson County, West Virginia. Water samples from multiple sites showing elevated fecal coliform counts were...
Authors
W. Bane Schill, Deborah D. Iwanowicz
By
Ecosystems Mission Area, Eastern Ecological Science Center

Detecting the undetectable: Characterization, optimization, and validation of an eDNA detection assay for the federally endangered dwarf wedgemussel, Alasmidonta heterodon (Bivalvia: Unionoida) Detecting the undetectable: Characterization, optimization, and validation of an eDNA detection assay for the federally endangered dwarf wedgemussel, Alasmidonta heterodon (Bivalvia: Unionoida)

Environmental (e)DNA assays are valuable tools for monitoring presence and distribution of cryptic species. Like many freshwater mussels, the dwarf wedgemussel, Alasmidonta heterodon numbers have dwindled and its range has diminished. As of its listing in 1993, only 10 to 20 locations were known to persist of the 70 Atlantic slope locations known historically. A qPCR assay to detect the...
Authors
W. Bane Schill, Heather S. Galbraith
By
Ecosystems Mission Area, Eastern Ecological Science Center

Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus) Establishing molecular methods to quantitatively profile gastric diet items of fish—Application to the invasive blue catfish (ictalurus furcatus)

Understanding the diet of invasive species helps researchers to more accurately assess the health, survivorship, growth, and stability of an invasive fish species, as well as their effects on native populations. Techniques capable of identifying multiple prey species from fish stomach contents have been developed. In this study, a multi-locus metabarcoding approach was used to identify...
Authors
Deborah D. Iwanowicz, W. Bane Schill, Lakyn R. Sanders, Tim Groves, Mary C. Groves
By
Ecosystems Mission Area, Eastern Ecological Science Center, Chesapeake Bay Activities

The complete maternal mitochondrial genome sequences of two imperiled North American freshwater mussels: Alasmidonta heterodon and Alasmidonta varicosa (Bivalvia: Unionoida: Unionidae) The complete maternal mitochondrial genome sequences of two imperiled North American freshwater mussels: Alasmidonta heterodon and Alasmidonta varicosa (Bivalvia: Unionoida: Unionidae)

The freshwater mussels Alasmidonta heterodon and A. varicosa historically inhabited rivers along the North American Atlantic coast from the Carolinas, U.S.A., to New Brunswick, CA. However, many populations have been extirpated, and A. heterodon is now federally listed in the U.S.A. as endangered, and both A. heterodon and A. varicosa are listed as vulnerable on the IUCN Red List. To...
Authors
Aaron Aunins, Cheryl L. Morrison, Heather Galbraith, Michael S. Eackles, W. Bane Schill, Tim L. King
By
Ecosystems Mission Area, Eastern Ecological Science Center

Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey Endozoicomonas dominates the gill and intestinal content microbiomes of Mytilus edulis from Barnegat Bay, New Jersey

Blue mussels, Mytilus edulis, Linnaeus 1758 from southern Barnegat Bay, New Jersey were examined to determine the make-up of the normal blue mussel microbiome. Sequencing of 16S ribosomal DNA amplicons from gill and intestinal content microbiomes using the Illumina® MiSeq platform yielded 1,276,161 paired end sequence reads from the gill libraries and 1,092,333 paired end sequence reads...
Authors
William B. Schill, Deborah D. Iwanowicz, Cynthia R. Adams
By
Ecosystems Mission Area, Eastern Ecological Science Center

Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has...
Authors
Deborah D. Iwanowicz, William B. Schill, Deanna H. Olson, M. J. Adams, Christine L. Densmore, Robert S. Cornman, Cynthia R. Adams, Chester Figiel, Chauncey W. Anderson, Andrew R Blaustein, Tara Chestnut
By
Ecosystems Mission Area, Water Resources Mission Area, Eastern Ecological Science Center, Forest and Rangeland Ecosystem Science Center, Oregon Water Science Center

*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government

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