Donald Tillitt, PhD (Former Employee)
Science and Products
Baseline fish health metrics in at-risk habitats critical for pallid sturgeon (Scaphirhynchus albus) in the Upper Missouri River Basin and bull trout (Salvelinus confluentus) in the Flathead River, Montana, 2017-18 Baseline fish health metrics in at-risk habitats critical for pallid sturgeon (Scaphirhynchus albus) in the Upper Missouri River Basin and bull trout (Salvelinus confluentus) in the Flathead River, Montana, 2017-18
Baseline fish health information on pallid sturgeon and bull trout or appropriate surrogate species (shovelnose sturgeon, cutthroat trout, mountain whitefish) in critical habitats with elevated risks of oil spills. Shovelnose sturgeon (Scaphirhynchus platorynchus) were collected in three locations near the confluence of the Missouri and Yellowstone Rivers and cutthroat trout...
Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
USGS Great Lakes lake trout egg thiamine monitoring data release USGS Great Lakes lake trout egg thiamine monitoring data release
Monitoring of vitamin B1 (thiamine) in the eggs of lake trout (Salvelinus namaycush) collected from lakes Huron and Michigan has been conducted by the U.S. Geological Survey since the mid-1990?s. The results of this monitoring effort have been used by natural resource management agencies and native American tribes within the Great Lakes basin to evaluate the reproductive health of lake...
Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)
Acute toxicity and clotting times of anticoagulant rodenticides of four fish species
Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data
We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate...
Filter Total Items: 215
Thiamine supplementation improves survival and body condition of hatchery-reared steelhead (Oncorhynchus mykiss) in Oregon Thiamine supplementation improves survival and body condition of hatchery-reared steelhead (Oncorhynchus mykiss) in Oregon
Early rearing of steelhead (Oncorhynchus mykiss) in Oregon hatcheries is often problematic; fry can become emaciated and die during the period between hatch and first feed. Thiamine (vitamin B1) deficiency has caused early mortality in salmonids; however, the thiamine status of Oregon’s steelhead populations is unknown, to date. Of the 26 egg samples from three Oregon hatcheries in 2019...
Authors
Aimee N. Reed, Freya Elizabeth Rowland, Jennifer A. Krajcik, Donald E. Tillitt
Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio
Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality...
Authors
Cathy A. Richter, Allison N. Evans, Scott A. Heppell, James L. Zajicek, Donald E. Tillitt
Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples
Chemically activated luciferase expression (CALUX) cell bioassays are popular tools for assessing endocrine activity of chemicals such as certain environmental contaminants. Although activity equivalents can be obtained from CALUX analysis, directly comparing these equivalents to those obtained from analytical chemistry methods can be problematic because of the complexity of endocrine...
Authors
Jennifer C. Brennan, Abigail Henke, Robert Gale, Diane K. Nicks, Donald Tillitt
Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds
Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha
Authors
Jessica Kristin Leet, Cathy A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Kristin Leet, Justin Greer, Cathy A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John D. Hansen
Response to Gard et al.'s (2021) Comments on the Critical Review “Polychlorinated Biphenyl Tissue-Concentration Thresholds for Survival, Growth, and Reproduction in Fish” Response to Gard et al.'s (2021) Comments on the Critical Review “Polychlorinated Biphenyl Tissue-Concentration Thresholds for Survival, Growth, and Reproduction in Fish”
This response is offered to the critique by Gard et al. (2021) of our meta-analysis of polychlorinated biphenyl (PCB)-induced toxicity data in fish (Berninger and Tillitt 2019). Gard et al. (2021) offered numerous comments, the most substantive suggesting that 1) we should have added no-observable–adverse effect residue (NOAER) data from additional studies and all data points from...
Authors
Jason P. Berninger, Donald E. Tillitt
Science and Products
Baseline fish health metrics in at-risk habitats critical for pallid sturgeon (Scaphirhynchus albus) in the Upper Missouri River Basin and bull trout (Salvelinus confluentus) in the Flathead River, Montana, 2017-18 Baseline fish health metrics in at-risk habitats critical for pallid sturgeon (Scaphirhynchus albus) in the Upper Missouri River Basin and bull trout (Salvelinus confluentus) in the Flathead River, Montana, 2017-18
Baseline fish health information on pallid sturgeon and bull trout or appropriate surrogate species (shovelnose sturgeon, cutthroat trout, mountain whitefish) in critical habitats with elevated risks of oil spills. Shovelnose sturgeon (Scaphirhynchus platorynchus) were collected in three locations near the confluence of the Missouri and Yellowstone Rivers and cutthroat trout...
Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20 Thiamine concentrations in lake trout eggs collected from the Great Lakes in 2019-20
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
USGS Great Lakes lake trout egg thiamine monitoring data release USGS Great Lakes lake trout egg thiamine monitoring data release
Monitoring of vitamin B1 (thiamine) in the eggs of lake trout (Salvelinus namaycush) collected from lakes Huron and Michigan has been conducted by the U.S. Geological Survey since the mid-1990?s. The results of this monitoring effort have been used by natural resource management agencies and native American tribes within the Great Lakes basin to evaluate the reproductive health of lake...
Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)
Acute toxicity and clotting times of anticoagulant rodenticides of four fish species
Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data
We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate...
Filter Total Items: 215
Thiamine supplementation improves survival and body condition of hatchery-reared steelhead (Oncorhynchus mykiss) in Oregon Thiamine supplementation improves survival and body condition of hatchery-reared steelhead (Oncorhynchus mykiss) in Oregon
Early rearing of steelhead (Oncorhynchus mykiss) in Oregon hatcheries is often problematic; fry can become emaciated and die during the period between hatch and first feed. Thiamine (vitamin B1) deficiency has caused early mortality in salmonids; however, the thiamine status of Oregon’s steelhead populations is unknown, to date. Of the 26 egg samples from three Oregon hatcheries in 2019...
Authors
Aimee N. Reed, Freya Elizabeth Rowland, Jennifer A. Krajcik, Donald E. Tillitt
Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio
Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality...
Authors
Cathy A. Richter, Allison N. Evans, Scott A. Heppell, James L. Zajicek, Donald E. Tillitt
Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples
Chemically activated luciferase expression (CALUX) cell bioassays are popular tools for assessing endocrine activity of chemicals such as certain environmental contaminants. Although activity equivalents can be obtained from CALUX analysis, directly comparing these equivalents to those obtained from analytical chemistry methods can be problematic because of the complexity of endocrine...
Authors
Jennifer C. Brennan, Abigail Henke, Robert Gale, Diane K. Nicks, Donald Tillitt
Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds
Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha
Authors
Jessica Kristin Leet, Cathy A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Kristin Leet, Justin Greer, Cathy A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John D. Hansen
Response to Gard et al.'s (2021) Comments on the Critical Review “Polychlorinated Biphenyl Tissue-Concentration Thresholds for Survival, Growth, and Reproduction in Fish” Response to Gard et al.'s (2021) Comments on the Critical Review “Polychlorinated Biphenyl Tissue-Concentration Thresholds for Survival, Growth, and Reproduction in Fish”
This response is offered to the critique by Gard et al. (2021) of our meta-analysis of polychlorinated biphenyl (PCB)-induced toxicity data in fish (Berninger and Tillitt 2019). Gard et al. (2021) offered numerous comments, the most substantive suggesting that 1) we should have added no-observable–adverse effect residue (NOAER) data from additional studies and all data points from...
Authors
Jason P. Berninger, Donald E. Tillitt
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government