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Donald Tillitt, PhD (Former Employee)

Science and Products

Frozen Lake Trout eggs being weighed for a thiaminase assay

Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
By
Ecosystems Mission Area, Species Management Research Program, Columbia Environmental Research Center
Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
Learn More
Filter Total Items: 14
No Result Found
Filter Total Items: 217

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Anticoagulant rodenticides (ARs) areused in rateradication efforts on island wildlife refuges. ARbait pellets can get into coralreefareasduring broadcasting and leadto exposure ofnon-target organisms, such as marine fishes. The objective of this study was to determine the sensitivity of representative saltwater fishes, Red-toothed triggerfish (Odonus niger) and Black triggerfish (Melichthys...
Authors
Rachelle Riegerix, Mike Tanner, Robert W. Gale, Donald E. Tillitt
By
Ecosystems Mission Area, Columbia Environmental Research Center

Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol

Fetal/neonatal environmental estrogen exposures alter developmental programing of the prostate gland causing onset of diseases later in life. We have previously shown in vitro that exposures to 17β-estradiol (E2) and the endocrine disrupting chemical bisphenol A, at concentrations relevant to human exposure, cause an elevation of estrogen receptor α (Esr1) mRNA in primary cultures of...
Authors
Ramji K. Bhandari, Julia A. Taylor, Jennifer Sommerfeld-Sager, Donald E. Tillitt, William A. Ricke, Frederick S. vom Saal
By
Ecosystems Mission Area, Columbia Environmental Research Center

Atrazine induced transgenerational reproductive effects in medaka (Oryzias latipes) Atrazine induced transgenerational reproductive effects in medaka (Oryzias latipes)

Atrazine is presently one of the most abundantly used herbicides in the United States, and a common contaminant of natural water bodies and drinking waters in high-use areas. Dysregulation of reproductive processes has been demonstrated in atrazine exposed fish, including alteration of key endocrine pathways on hypothalamic-pituitary-gonadal (HPG) axis. However, the potential for...
Authors
Jacob A. Cleary, Donald E. Tillitt, Fredrick S. vom Saal, Diane Nicks, Rachel Claunch, Ramji K. Bhandari
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Bisphenol A and 17 alpha-ethinylestradiol-induced transgenerational differences in expression of osmoregulatory genes in the gill of medaka (Oryzias latipes) Bisphenol A and 17 alpha-ethinylestradiol-induced transgenerational differences in expression of osmoregulatory genes in the gill of medaka (Oryzias latipes)

Embryonic bisphenol A (BPA) and 17α-ethinylestradiol (EE2) exposure can have far reaching health effects in fish, including adult onset transgenerational reproductive abnormalities, anxiety, and cardiac disorders. It is unknown whether these two environmental estrogens can induce transgenerational abnormalities in the gill. The present study examined transgenerational effects of BPA or...
Authors
Xuegeng Wang, Diamond Hill, Donald E. Tillitt, Ramji K. Bhandari
By
Ecosystems Mission Area, Columbia Environmental Research Center

Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish

Polychlorinated biphenyls (PCBs) have left a legacy of environmental contamination. Even though they were banned from production and active use in the 1970s, they persist in the environment and still have the potential to impact aquatic life. Our objective was to identify data from controlled laboratory studies of PCB‐related adverse effects in fish and to conduct a meta‐analysis on...
Authors
Jason P. Berninger, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Thiamine deficiency in fishes: Causes, consequences, and potential solutions Thiamine deficiency in fishes: Causes, consequences, and potential solutions

Thiamine deficiency complex (TDC) is a disorder resulting from the inability to acquire or retain thiamine (vitamin B1) and has been documented in organisms in aquatic ecosystems ranging from the Baltic Sea to the Laurentian Great Lakes. The biological mechanisms leading to TDC emergence may vary among systems, but in fishes, one common outcome is high mortality among early life stages...
Authors
Avril M. Harder, William R. Ardren, Allison N. Evans, Matthew H. Futia, Clifford E. Kraft, J. Ellen Marsden, Catherine A. Richter, Jacques Rinchard, Donald E. Tillitt, Mark R. Christie
By
Ecosystems Mission Area, Columbia Environmental Research Center

Embryonic effects of an environmentally relevant PCB mixture in the domestic chicken Embryonic effects of an environmentally relevant PCB mixture in the domestic chicken

Studies were conducted to develop methods to assess the effects of a complex mixture of polychlorinated biphenyls (PCBs) in the domestic chicken (Gallus domesticus). Treatments were administered by egg injection to compare embryonic effects of an environmentally relevant PCB congener mixture in the domestic chicken over a range of doses. Chicken eggs were injected with the PCB mixture...
Authors
Mary Ann Ottinger, Emma T. Lavoie, Mary E. B. Bohannon, Allegra M. Marcel, Anna E. Tschiffely, Kara B. Duffy, Moira A. McKernan, Nichola Thompson, H. Kasen Whitehouse, Kimya Davani, Marci Strauss, Donald E. Tillitt, Joshua Lipton, Karen M. Dean
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Mapping elemental contamination on Palmyra Atoll National Wildlife Refuge Mapping elemental contamination on Palmyra Atoll National Wildlife Refuge

Palmyra Atoll, once a WWII U.S. Navy air station, is now a U.S. National Wildlife Refuge with nearly 50 km2 of coral reef and 275 ha of emergent lands with forests of Pisonia grandistrees and colonies of several bird species. Due to the known elemental and organic contamination from chemicals associated with aviation, power generation and transmission, waste management, and other air...
Authors
Matthew A. Struckhoff, Carl E. Orazio, Donald E. Tillitt, David K. Shaver, Diana M. Papoulias
By
Ecosystems Mission Area, Columbia Environmental Research Center

Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances

There is a need to adapt cell bioassays to 384-well and 1536-well formats instead of the traditional 96-well format as high-throughput screening (HTS) demands increase. However, the sensitivity and performance of the bioassay must be re-verified in these higher micro-well plates, and verification of cell health must also be HT (high-throughput). We have adapted two commonly used human...
Authors
Jennifer Brennan, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Grand challenges for integrated USGS science — A workshop report Grand challenges for integrated USGS science — A workshop report

Authors
Karen E. Jenni, Martin B. Goldhaber, Julio L. Betancourt, Jill S. Baron, Sky Bristol, Mary Cantrill, Paul E. Exter, Michael J. Focazio, John W. Haines, Lauren E. Hay, Leslie Hsu, Victor F. Labson, Kevin D. Lafferty, K. A. Ludwig, Paul C. D. Milly, Toni L. Morelli, Suzette A. Morman, Nedal T. Nassar, Timothy R. Newman, Andrea C. Ostroff, Jordan S. Read, Sasha C. Reed, Carl D. Shapiro, Richard A. Smith, Ward E. Sanford, Terry L. Sohl, Edward G. Stets, Adam J. Terando, Donald E. Tillitt, Michael A. Tischler, Patricia L. Toccalino, David J. Wald, Mark P. Waldrop, Anne Wein, Jake F. Weltzin, Christian E. Zimmerman
By
Science Synthesis, Analysis, and Research Program, Science Analytics and Synthesis (SAS), Science and Decisions Center, Earth Resources Observation and Science (EROS) Center, Land Use Land Cover Modeling

Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl

The aquatic food web of the Great Lakes has been contaminated with polychlorinated biphenyls (PCBs) since the mid-20th century. Threats of PCB exposures to long-lived species of fish, such as lake sturgeon (Acipenser fulvescens), have been uncertain because of a lack of information on the relative sensitivity of the species. The objective of the present study was to evaluate the...
Authors
Donald E. Tillitt, Justin A. Buckler, Diane Nicks, James S. Candrl, Rachel Claunch, Robert W. Gale, Holly J. Puglis, Edward E. Little, Tiffany L. Linbo, Mary Baker
By
Ecosystems Mission Area, Columbia Environmental Research Center

Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)

Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression...
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Columbia Environmental Research Center

Science and Products

Frozen Lake Trout eggs being weighed for a thiaminase assay

Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
By
Ecosystems Mission Area, Species Management Research Program, Columbia Environmental Research Center
Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

Thiamine (Vitamin B1) Deficiency in Fish and Wildlife

In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
Learn More
Filter Total Items: 14
No Result Found
Filter Total Items: 217

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides) Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Anticoagulant rodenticides (ARs) areused in rateradication efforts on island wildlife refuges. ARbait pellets can get into coralreefareasduring broadcasting and leadto exposure ofnon-target organisms, such as marine fishes. The objective of this study was to determine the sensitivity of representative saltwater fishes, Red-toothed triggerfish (Odonus niger) and Black triggerfish (Melichthys...
Authors
Rachelle Riegerix, Mike Tanner, Robert W. Gale, Donald E. Tillitt
By
Ecosystems Mission Area, Columbia Environmental Research Center

Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol

Fetal/neonatal environmental estrogen exposures alter developmental programing of the prostate gland causing onset of diseases later in life. We have previously shown in vitro that exposures to 17β-estradiol (E2) and the endocrine disrupting chemical bisphenol A, at concentrations relevant to human exposure, cause an elevation of estrogen receptor α (Esr1) mRNA in primary cultures of...
Authors
Ramji K. Bhandari, Julia A. Taylor, Jennifer Sommerfeld-Sager, Donald E. Tillitt, William A. Ricke, Frederick S. vom Saal
By
Ecosystems Mission Area, Columbia Environmental Research Center

Atrazine induced transgenerational reproductive effects in medaka (Oryzias latipes) Atrazine induced transgenerational reproductive effects in medaka (Oryzias latipes)

Atrazine is presently one of the most abundantly used herbicides in the United States, and a common contaminant of natural water bodies and drinking waters in high-use areas. Dysregulation of reproductive processes has been demonstrated in atrazine exposed fish, including alteration of key endocrine pathways on hypothalamic-pituitary-gonadal (HPG) axis. However, the potential for...
Authors
Jacob A. Cleary, Donald E. Tillitt, Fredrick S. vom Saal, Diane Nicks, Rachel Claunch, Ramji K. Bhandari
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Bisphenol A and 17 alpha-ethinylestradiol-induced transgenerational differences in expression of osmoregulatory genes in the gill of medaka (Oryzias latipes) Bisphenol A and 17 alpha-ethinylestradiol-induced transgenerational differences in expression of osmoregulatory genes in the gill of medaka (Oryzias latipes)

Embryonic bisphenol A (BPA) and 17α-ethinylestradiol (EE2) exposure can have far reaching health effects in fish, including adult onset transgenerational reproductive abnormalities, anxiety, and cardiac disorders. It is unknown whether these two environmental estrogens can induce transgenerational abnormalities in the gill. The present study examined transgenerational effects of BPA or...
Authors
Xuegeng Wang, Diamond Hill, Donald E. Tillitt, Ramji K. Bhandari
By
Ecosystems Mission Area, Columbia Environmental Research Center

Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish

Polychlorinated biphenyls (PCBs) have left a legacy of environmental contamination. Even though they were banned from production and active use in the 1970s, they persist in the environment and still have the potential to impact aquatic life. Our objective was to identify data from controlled laboratory studies of PCB‐related adverse effects in fish and to conduct a meta‐analysis on...
Authors
Jason P. Berninger, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Thiamine deficiency in fishes: Causes, consequences, and potential solutions Thiamine deficiency in fishes: Causes, consequences, and potential solutions

Thiamine deficiency complex (TDC) is a disorder resulting from the inability to acquire or retain thiamine (vitamin B1) and has been documented in organisms in aquatic ecosystems ranging from the Baltic Sea to the Laurentian Great Lakes. The biological mechanisms leading to TDC emergence may vary among systems, but in fishes, one common outcome is high mortality among early life stages...
Authors
Avril M. Harder, William R. Ardren, Allison N. Evans, Matthew H. Futia, Clifford E. Kraft, J. Ellen Marsden, Catherine A. Richter, Jacques Rinchard, Donald E. Tillitt, Mark R. Christie
By
Ecosystems Mission Area, Columbia Environmental Research Center

Embryonic effects of an environmentally relevant PCB mixture in the domestic chicken Embryonic effects of an environmentally relevant PCB mixture in the domestic chicken

Studies were conducted to develop methods to assess the effects of a complex mixture of polychlorinated biphenyls (PCBs) in the domestic chicken (Gallus domesticus). Treatments were administered by egg injection to compare embryonic effects of an environmentally relevant PCB congener mixture in the domestic chicken over a range of doses. Chicken eggs were injected with the PCB mixture...
Authors
Mary Ann Ottinger, Emma T. Lavoie, Mary E. B. Bohannon, Allegra M. Marcel, Anna E. Tschiffely, Kara B. Duffy, Moira A. McKernan, Nichola Thompson, H. Kasen Whitehouse, Kimya Davani, Marci Strauss, Donald E. Tillitt, Joshua Lipton, Karen M. Dean
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Mapping elemental contamination on Palmyra Atoll National Wildlife Refuge Mapping elemental contamination on Palmyra Atoll National Wildlife Refuge

Palmyra Atoll, once a WWII U.S. Navy air station, is now a U.S. National Wildlife Refuge with nearly 50 km2 of coral reef and 275 ha of emergent lands with forests of Pisonia grandistrees and colonies of several bird species. Due to the known elemental and organic contamination from chemicals associated with aviation, power generation and transmission, waste management, and other air...
Authors
Matthew A. Struckhoff, Carl E. Orazio, Donald E. Tillitt, David K. Shaver, Diana M. Papoulias
By
Ecosystems Mission Area, Columbia Environmental Research Center

Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances

There is a need to adapt cell bioassays to 384-well and 1536-well formats instead of the traditional 96-well format as high-throughput screening (HTS) demands increase. However, the sensitivity and performance of the bioassay must be re-verified in these higher micro-well plates, and verification of cell health must also be HT (high-throughput). We have adapted two commonly used human...
Authors
Jennifer Brennan, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

Grand challenges for integrated USGS science — A workshop report Grand challenges for integrated USGS science — A workshop report

Authors
Karen E. Jenni, Martin B. Goldhaber, Julio L. Betancourt, Jill S. Baron, Sky Bristol, Mary Cantrill, Paul E. Exter, Michael J. Focazio, John W. Haines, Lauren E. Hay, Leslie Hsu, Victor F. Labson, Kevin D. Lafferty, K. A. Ludwig, Paul C. D. Milly, Toni L. Morelli, Suzette A. Morman, Nedal T. Nassar, Timothy R. Newman, Andrea C. Ostroff, Jordan S. Read, Sasha C. Reed, Carl D. Shapiro, Richard A. Smith, Ward E. Sanford, Terry L. Sohl, Edward G. Stets, Adam J. Terando, Donald E. Tillitt, Michael A. Tischler, Patricia L. Toccalino, David J. Wald, Mark P. Waldrop, Anne Wein, Jake F. Weltzin, Christian E. Zimmerman
By
Science Synthesis, Analysis, and Research Program, Science Analytics and Synthesis (SAS), Science and Decisions Center, Earth Resources Observation and Science (EROS) Center, Land Use Land Cover Modeling

Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl

The aquatic food web of the Great Lakes has been contaminated with polychlorinated biphenyls (PCBs) since the mid-20th century. Threats of PCB exposures to long-lived species of fish, such as lake sturgeon (Acipenser fulvescens), have been uncertain because of a lack of information on the relative sensitivity of the species. The objective of the present study was to evaluate the...
Authors
Donald E. Tillitt, Justin A. Buckler, Diane Nicks, James S. Candrl, Rachel Claunch, Robert W. Gale, Holly J. Puglis, Edward E. Little, Tiffany L. Linbo, Mary Baker
By
Ecosystems Mission Area, Columbia Environmental Research Center

Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)

Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression...
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Columbia Environmental Research Center

*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government

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