Donald Tillitt, PhD (Former Employee)
Science and Products
Filter Total Items: 215
Quantitative polymerase chain reaction (PCR) assays for a bacterial thiaminase I gene and the thiaminase-producing bacterium Paenibacillus thiaminolyticus. Quantitative polymerase chain reaction (PCR) assays for a bacterial thiaminase I gene and the thiaminase-producing bacterium Paenibacillus thiaminolyticus.
The thiaminase I enzyme produced by the gram-positive bacterium Paenibacillus thiaminolyticus isolated from the viscera of Lake Michigan alewives Alosa pseudoharengus is currently the only defined source of the thiaminase activity linked to thiamine (vitamin B1) deficiency in early mortality syndrome (EMS) in the larvae of Great Lakes salmonines. Diets of alewife or isolated strains of P
Authors
C.A. Richter, Maureen K. Wright-Osment, J.L. Zajicek, D. C. Honeyfield, D. E. Tillitt
Variations of thiaminase I activity pH dependencies among typical Great Lakes forage fish and Paenibacillus thiaminolyticus. Variations of thiaminase I activity pH dependencies among typical Great Lakes forage fish and Paenibacillus thiaminolyticus.
The source of thiaminase in the Great Lakes food web remains unknown. Biochemical characterization of the thiaminase I activities observed in forage fish was undertaken to provide insights into potential thiaminase sources and to optimize catalytic assay conditions. We measured the thiaminase I activities of crude extracts from five forage fish species and one strain of Paenibacillus
Authors
J.L. Zajicek, L. Brown, S.B. Brown, D. C. Honeyfield, J.D. Fitzsimons, D. E. Tillitt
Dreissenid mussels from the Great Lakes contain elevated thiaminase activity Dreissenid mussels from the Great Lakes contain elevated thiaminase activity
We examined thiaminase activity in dreissenid mussels collected at different depths and seasons, and from various locations in Lakes Michigan, Ontario, and Huron. Here we present evidence that two dreissenid mussel species (Dreissena bugensis and D. polymorpha) contain thiaminase activity that is 5-100 fold greater than observed in Great Lakes fishes. Thiaminase activity in zebra mussels...
Authors
D. E. Tillitt, S.C. Riley, A.N. Evans, S. J. Nichols, J.L. Zajicek, J. Rinchard, C.A. Richter, C.C. Krueger
The genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute exposure to the androgen, 17β-trenbolone The genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute exposure to the androgen, 17β-trenbolone
We investigated the genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute (4 days) exposure to 0.1 or 1.0 ??g/L of 17??-trenbolone (TB), the active metabolite of an anabolic androgenic steroid used as a growth promoter in cattle and a contaminant of concern in aquatic systems. Our objectives were to investigate the gene expression profile induced by...
Authors
Jennifer Dorts, Cathy A. Richter, Maureen K. Wright-Osment, Mark R. Ellersieck, Barbara J. Carter, Donald E. Tillitt
Thiamine deficiency effects on the vision and foraging ability of lake trout fry Thiamine deficiency effects on the vision and foraging ability of lake trout fry
The exact causes of the historical recruitment failures of Great Lakes lake trout Salvelinus namaycush are unknown. Thiamine deficiency has been associated with neurological abnormalities in lake trout that lead to early mortality syndrome (EMS) in salmonine swim-up fry, and EMS-related mortality at the swim-up stage is a factor that contributes to the reproductive failure of lake trout
Authors
Donald E. Tillitt, James L. Zajicek, Rachel Claunch, Dale C. Honeyfield, John D. Fitzsimons, Scott B. Brown
Reproductive impairment of Great Lakes lake trout by dioxin-like chemicals: Chapter 21 Reproductive impairment of Great Lakes lake trout by dioxin-like chemicals: Chapter 21
No abstract available.
Authors
Donald E. Tillitt, Philip S. Cook, John P. Giesy
Relations between and among contaminant concentrations and biomarkers in black bass (Micropterus spp.) and common carp (Cyprinus carpio) from large U.S. rivers, 1995-2004 Relations between and among contaminant concentrations and biomarkers in black bass (Micropterus spp.) and common carp (Cyprinus carpio) from large U.S. rivers, 1995-2004
Environmental contaminant and biomarker monitoring data from major U.S. river basins were summarized for black bass (Micropterus spp.) and common carp (Cyprinus carpio) sampled over a nine year period. Cumulative frequency distributions revealed taxon differences for many organochlorine residue concentrations, elemental contaminant concentrations, and biomarkers, but few gender...
Authors
J.E. Hinck, C. J. Schmitt, Mark R. Ellersieck, D. E. Tillitt
Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa) Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa)
We examined the sensitivity of the wood duck (Aix sponsa) embryo to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by injecting the toxicant into their eggs. Six groups of wood duck eggs (n = 35 to 211 per trial) were injected with 0 to 4600 pg TCDD/g egg between 2003 and 2005. Injections were made into yolk prior to incubation, and eggs were subsequently incubated and assessed weekly for...
Authors
T.P. Augspurger, D. E. Tillitt, S.J. Bursian, S.D. Fitzgerald, D.E. Hinton, R.T. Di Giulio
Accumulation of environmental contaminants in wood duck (Aix sponsa) eggs, with emphasis on polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans Accumulation of environmental contaminants in wood duck (Aix sponsa) eggs, with emphasis on polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans
We measured polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), organochlorine pesticides, and mercury in wood duck (Aix sponsa) eggs collected near a North Carolina (USA) bleached kraft paper mill. Samples were taken a decade after the mill stopped using molecular chlorine. Using avian toxic equivalency factors, 2,3,7,8
Authors
T.P. Augspurger, K. R. Echols, P.H. Peterman, T.W. May, C.E. Orazio, D. E. Tillitt, R.T. Di Giulio
Chemical contaminants, health indicators, and reproductive biomarker responses in fish from rivers in the Southeastern United States Chemical contaminants, health indicators, and reproductive biomarker responses in fish from rivers in the Southeastern United States
Largemouth bass (Micropterus salmoides) and common carp (Cyprinus carpio) were collected from 13 sites located in the Mobile (MRB), Apalachicola–Flint–Chattahoochee (ARB), Savannah (SRB), and Pee Dee (PRB) River Basins to document spatial trends in accumulative chemical contaminants, health indicators, and reproductive biomarkers. Organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p...
Authors
J.E. Hinck, V. S. Blazer, N. D. Denslow, K. R. Echols, R.W. Gale, C. Wieser, T.W. May, M. Ellersieck, J.J. Coyle, D. E. Tillitt
Use of a 15 k gene microarray to determine gene expression changes in response to acute and chronic methylmercury exposure in the fathead minnow Pimephales promelas Rafinesque Use of a 15 k gene microarray to determine gene expression changes in response to acute and chronic methylmercury exposure in the fathead minnow Pimephales promelas Rafinesque
This study describes the use of a 15 000 gene microarray developed for the toxicological model species, Pimephales promelas, in investigating the impact of acute and chronic methylmercury exposures in male gonad and liver tissues. The results show significant differences in the individual genes that were differentially expressed in response to each treatment. In liver, a total of 650...
Authors
R. Klaper, Barbara J. Carter, C.A. Richter, P.E. Drevnick, M.B. Sandheinrich, D. E. Tillitt
Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI
Fry mortality which was first observed in the late 1960s in Great Lakes salmonines and in Baltic Sea salmon in 1974 has now been linked to thiamine deficiency (historically referred to as Early Mortality Syndrome, or EMS and M74, respectively). Over the past 14 years significant strides have been made in our understanding of this perplexing problem. It is now known that thiamine...
Authors
Dale C. Honeyfield, Donald E. Tillitt, Stephen C. Riley
Science and Products
Filter Total Items: 215
Quantitative polymerase chain reaction (PCR) assays for a bacterial thiaminase I gene and the thiaminase-producing bacterium Paenibacillus thiaminolyticus. Quantitative polymerase chain reaction (PCR) assays for a bacterial thiaminase I gene and the thiaminase-producing bacterium Paenibacillus thiaminolyticus.
The thiaminase I enzyme produced by the gram-positive bacterium Paenibacillus thiaminolyticus isolated from the viscera of Lake Michigan alewives Alosa pseudoharengus is currently the only defined source of the thiaminase activity linked to thiamine (vitamin B1) deficiency in early mortality syndrome (EMS) in the larvae of Great Lakes salmonines. Diets of alewife or isolated strains of P
Authors
C.A. Richter, Maureen K. Wright-Osment, J.L. Zajicek, D. C. Honeyfield, D. E. Tillitt
Variations of thiaminase I activity pH dependencies among typical Great Lakes forage fish and Paenibacillus thiaminolyticus. Variations of thiaminase I activity pH dependencies among typical Great Lakes forage fish and Paenibacillus thiaminolyticus.
The source of thiaminase in the Great Lakes food web remains unknown. Biochemical characterization of the thiaminase I activities observed in forage fish was undertaken to provide insights into potential thiaminase sources and to optimize catalytic assay conditions. We measured the thiaminase I activities of crude extracts from five forage fish species and one strain of Paenibacillus
Authors
J.L. Zajicek, L. Brown, S.B. Brown, D. C. Honeyfield, J.D. Fitzsimons, D. E. Tillitt
Dreissenid mussels from the Great Lakes contain elevated thiaminase activity Dreissenid mussels from the Great Lakes contain elevated thiaminase activity
We examined thiaminase activity in dreissenid mussels collected at different depths and seasons, and from various locations in Lakes Michigan, Ontario, and Huron. Here we present evidence that two dreissenid mussel species (Dreissena bugensis and D. polymorpha) contain thiaminase activity that is 5-100 fold greater than observed in Great Lakes fishes. Thiaminase activity in zebra mussels...
Authors
D. E. Tillitt, S.C. Riley, A.N. Evans, S. J. Nichols, J.L. Zajicek, J. Rinchard, C.A. Richter, C.C. Krueger
The genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute exposure to the androgen, 17β-trenbolone The genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute exposure to the androgen, 17β-trenbolone
We investigated the genomic transcriptional response of female fathead minnows (Pimephales promelas) to an acute (4 days) exposure to 0.1 or 1.0 ??g/L of 17??-trenbolone (TB), the active metabolite of an anabolic androgenic steroid used as a growth promoter in cattle and a contaminant of concern in aquatic systems. Our objectives were to investigate the gene expression profile induced by...
Authors
Jennifer Dorts, Cathy A. Richter, Maureen K. Wright-Osment, Mark R. Ellersieck, Barbara J. Carter, Donald E. Tillitt
Thiamine deficiency effects on the vision and foraging ability of lake trout fry Thiamine deficiency effects on the vision and foraging ability of lake trout fry
The exact causes of the historical recruitment failures of Great Lakes lake trout Salvelinus namaycush are unknown. Thiamine deficiency has been associated with neurological abnormalities in lake trout that lead to early mortality syndrome (EMS) in salmonine swim-up fry, and EMS-related mortality at the swim-up stage is a factor that contributes to the reproductive failure of lake trout
Authors
Donald E. Tillitt, James L. Zajicek, Rachel Claunch, Dale C. Honeyfield, John D. Fitzsimons, Scott B. Brown
Reproductive impairment of Great Lakes lake trout by dioxin-like chemicals: Chapter 21 Reproductive impairment of Great Lakes lake trout by dioxin-like chemicals: Chapter 21
No abstract available.
Authors
Donald E. Tillitt, Philip S. Cook, John P. Giesy
Relations between and among contaminant concentrations and biomarkers in black bass (Micropterus spp.) and common carp (Cyprinus carpio) from large U.S. rivers, 1995-2004 Relations between and among contaminant concentrations and biomarkers in black bass (Micropterus spp.) and common carp (Cyprinus carpio) from large U.S. rivers, 1995-2004
Environmental contaminant and biomarker monitoring data from major U.S. river basins were summarized for black bass (Micropterus spp.) and common carp (Cyprinus carpio) sampled over a nine year period. Cumulative frequency distributions revealed taxon differences for many organochlorine residue concentrations, elemental contaminant concentrations, and biomarkers, but few gender...
Authors
J.E. Hinck, C. J. Schmitt, Mark R. Ellersieck, D. E. Tillitt
Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa) Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa)
We examined the sensitivity of the wood duck (Aix sponsa) embryo to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by injecting the toxicant into their eggs. Six groups of wood duck eggs (n = 35 to 211 per trial) were injected with 0 to 4600 pg TCDD/g egg between 2003 and 2005. Injections were made into yolk prior to incubation, and eggs were subsequently incubated and assessed weekly for...
Authors
T.P. Augspurger, D. E. Tillitt, S.J. Bursian, S.D. Fitzgerald, D.E. Hinton, R.T. Di Giulio
Accumulation of environmental contaminants in wood duck (Aix sponsa) eggs, with emphasis on polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans Accumulation of environmental contaminants in wood duck (Aix sponsa) eggs, with emphasis on polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans
We measured polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), organochlorine pesticides, and mercury in wood duck (Aix sponsa) eggs collected near a North Carolina (USA) bleached kraft paper mill. Samples were taken a decade after the mill stopped using molecular chlorine. Using avian toxic equivalency factors, 2,3,7,8
Authors
T.P. Augspurger, K. R. Echols, P.H. Peterman, T.W. May, C.E. Orazio, D. E. Tillitt, R.T. Di Giulio
Chemical contaminants, health indicators, and reproductive biomarker responses in fish from rivers in the Southeastern United States Chemical contaminants, health indicators, and reproductive biomarker responses in fish from rivers in the Southeastern United States
Largemouth bass (Micropterus salmoides) and common carp (Cyprinus carpio) were collected from 13 sites located in the Mobile (MRB), Apalachicola–Flint–Chattahoochee (ARB), Savannah (SRB), and Pee Dee (PRB) River Basins to document spatial trends in accumulative chemical contaminants, health indicators, and reproductive biomarkers. Organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p...
Authors
J.E. Hinck, V. S. Blazer, N. D. Denslow, K. R. Echols, R.W. Gale, C. Wieser, T.W. May, M. Ellersieck, J.J. Coyle, D. E. Tillitt
Use of a 15 k gene microarray to determine gene expression changes in response to acute and chronic methylmercury exposure in the fathead minnow Pimephales promelas Rafinesque Use of a 15 k gene microarray to determine gene expression changes in response to acute and chronic methylmercury exposure in the fathead minnow Pimephales promelas Rafinesque
This study describes the use of a 15 000 gene microarray developed for the toxicological model species, Pimephales promelas, in investigating the impact of acute and chronic methylmercury exposures in male gonad and liver tissues. The results show significant differences in the individual genes that were differentially expressed in response to each treatment. In liver, a total of 650...
Authors
R. Klaper, Barbara J. Carter, C.A. Richter, P.E. Drevnick, M.B. Sandheinrich, D. E. Tillitt
Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI
Fry mortality which was first observed in the late 1960s in Great Lakes salmonines and in Baltic Sea salmon in 1974 has now been linked to thiamine deficiency (historically referred to as Early Mortality Syndrome, or EMS and M74, respectively). Over the past 14 years significant strides have been made in our understanding of this perplexing problem. It is now known that thiamine...
Authors
Dale C. Honeyfield, Donald E. Tillitt, Stephen C. Riley
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government