James Luoma
Science and Products
Avoidance behavior of cold-, cool-, and warmwater fish exposed to Zequanox in a two-choice preference chamber
Zebra (Dreissenia polymorpha, Pallas 1771) and quagga (D. bugensis, Andrusov 1897) mussels, collectively referred to as dreissenid mussels, are invasive bivalves native to the Ponto-Caspian region of Eurasia (Stepien et al. 2013; Benson 2018a). High fecundity and a free-swimming planktonic life stage allow for easy and rapid dispersal of dreissenid mussels (Mackie 1991; Marsden et al. 2013)...
Assessment of Open Water Zequanox Applications for Controlling Dreissenid Mussels within an Inland Lake
Invasion of dreissenid mussels (zebra and quagga mussels, Dreissena polymorpha and D. rostriformis bugensis, respectively) into the Great Lakes and Mississippi River Basins has resulted in estimated economic impacts as high as $1 billion annually for maintenance and repair of biofouled water conveyance systems and other infrastructures (Pimentel et al. 2005).
Invasive Mussel Control Science: Management Tools for Assessing the Risks and Control of Invasive Dreissenid Species
Invasive zebra and quagga mussels (Dreissena polymorpha and D. rostriformis bugensis, respectively) are causing significant ecological and economic impacts and the scope of these impacts increases as they continue to spread across North America. The USGS conducts science to inform management actions for controlling and mitigating the impacts of invasive mussels. Studies include evaluation and...
Use of electrified fields to control dreissenid mussels
Zebra and quagga mussels were first introduced into the Great Lakes in the 1980’s and they have since expanded to over 750 inland lakes in addition to the 5 Great Lakes (http://fl.biology.usgs.gov/Nonindigenous_Species/Zebra_mussel_distribution/zebra_mussel_distribution.html, accessed 8/5/2015). A 2009 study conducted by the Idaho Aquatic Nuisance Species Taskforce estimated the annual economic...
Exposure-Related Effects of Zequanox on Lake Sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush) Survival and Condition
A dead-cell, spray-dried powder formulation of Pseudomonas fluorescens, strain CL145A was recently approved by the US Environmental Protection Agency (EPA) for control of dreissenid mussels (zebra mussel, Dreissena polymorpha and quagga mussel, Dreissena rostriformis bugensis) in open-water environments. The EPA approved product, Zequanox® (registration number 84059-15) is manufactured by Marrone...
Temperature-dependent toxicity of molluscicides to zebra mussels
Zebra mussels (Dreissenia polymorpha) are native to the Black, Caspian, and Aral Seas of eastern Europe (Gollasch and Leppäkoski 1999) and they were likely introduced into Lake Erie as veliger larvae in the summer or fall of 1985 (Hebert et al. 1989). Their high reproductive capacity and planktonic larval stage enable zebra mussels to rapidly disperse (Birnbaum 2011). Less than 10 years after...
Field Evaluation of an Improved Solid TFM Formulation for Use in Treating Small Tributary Streams, data
This study builds upon work conducted under the Great Lakes Fishery Commission (GLFC) Technical Assistance Program that was funded to prepare and evaluate an alternative solid formulation of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) in a laboratory flume. The project aligns within the lampricide theme in the Sea Lamprey Research Program by improving lampricide performance, efficacy, and
Determination of the seasonality effect on sea lamprey and TFM efficacy - Year One
Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with of 4-Nitro-3-(trifluoromethyl)phenol (TFM, a lampricide) is essential to reducing the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of treating streams with lampricide is ensuring that the proper amount of TFM is applied, effectively co
Avoidance behavior of cold-, cool-, and warmwater fish exposed to Zequanox in a two-choice preference chamber, data release
Zequanox® is an EPA-registered biopesticide for controlling populations of dreissenid mussels
(zebra and quagga mussels). Zequanox has demonstrated selective toxicity toward dreissenid mussels; however, a recent laboratory study indicated the potential for Zequanox to impact a non-target salmonid species. We assessed the potential exposure risk by observing the avoidance behavior of two re
Assessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality, data release
This dataset captures the Zebra Mussel dose-response to four independent toxicants and each binary mixture of those toxicants. Toxicants included EarthTec QZ, Clam-Trol CT-2 (syn. Spectrus CT1300), niclosamide, and potassium chloride. Each dose of toxicant was verified with either ICP-OES or UHPLC, depending on analyte, and comparison to a standard curve. Mortality was tabulated for each observati
Assessment of uncontained Zequanox applications in a Midwestern lake data
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included
Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush) Data
The environmental fate, persistence, and point-source discharge of traditional molluscicidal compounds led to the development of an alternative biomolluscicide, Zequanox. Previous studies evaluated the efficacy and non-target animal safety of Zequanox in laboratory, mesocosm, and field enclosure studies. One study indicated sensitivity of salmonid species and lake sturgeon (Acipenser fulvescens Ra
Filter Total Items: 25
Concentration addition and independent action assessments of the binary mixtures of four toxicants on zebra mussel (Dreissena polymorpha) mortality
Researchers most often focus on individual toxicants when identifying effective chemical control agents for aquatic invasive species; however, toxicant mixtures may elicit synergistic effects. Synergistic effects may decrease required concentrations and shorten exposure durations for treatments. We investigated four toxicants (EarthTec QZ, Clam-Trol CT-2, niclosamide, and potassium chloride) that
Field evaluation of an improved solid TFM formulation for use in treating small tributary streams
A solid lampricide formulation containing 23% 3-trifluoromethyl-4-nitrophenol (TFM) as the active ingredient was developed in the mid-1980s for use in small tributaries of dendritic streams during routine treatments to kill larval sea lamprey. This TFM bar formulation was designed to use a matrix of commercially prepared surfactants that would dissolve and slowly release their TFM payload over an
Avoidance of cold-, cool-, and warm-water fishes to Zequanox® exposure
Zequanox® is a biopesticide registered by the U.S. Environmental Protection Agency (USEPA) and the Canadian Pest Management Regulatory Agency for controlling dreissenid mussels with demonstrated selective toxicity. However, some research has indicated that Zequanox may impact the body condition and survival of some non-target species. We assessed avoidance behaviors of two species of cold-, cool-,
Development and evaluation of an improved TFM formulation for use in feeder stream treatments
The binational Great Lakes Fishery Commission sponsored Sea Lamprey Control Program effectively utilizes a variety of lampricide tools to keep populations of parasitic sea lampreys in the Great Lakes at levels that do not cause undue economic or ecological damage. The most widely used toxicant used in lampricide formulations is 3-trifluoromethyl-4-nitrophenol (TFM). In typical treatments, a liquid
Assessment of uncontained Zequanox applications for zebra mussel control in a Midwestern lake
Zebra mussels (Dreissena polymorpha) are invasive bivalves that have perturbed aquatic ecosystems within North America since their introduction in the mid-1980s. Control of zebra mussels has largely been restricted to raw water conveyance systems and associated infrastructures because few control products are registered for application in surface waters. The biopesticide Zequanox was registered in
Optimum electrofishing waveforms and parameters to induce a capture-prone response in juvenile Grass Carp
Grass Carp (Ctenopharyngodon idella) are a non-native species to North America that were first introduced for vegetation control in the 1960s. However, wild-reproducing Grass Carp can negatively impact aquatic habitats and aquatic communities by consuming substantial amounts of aquatic vegetation and increasing turbidity. Numerous fisheries techniques have been used in an attempt to control or e
Effects of temperature and exposure duration on four potential rapid-response tools for zebra mussel (Dreissena polymorpha) eradication
Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways by early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous er
A bioassay assessment of a zebra mussel (Dreissena polymorpha) eradication treatment
Zebra mussels (Dreissena polymorpha, Pallas, 1771) are an aquatic invasive species in theUnited States, and new infestations of zebra mussels can rapidly expand into dense colonies. Zebramussels were first reported in Marion Lake, Dakota County, Minnesota, in September 2017, andsurveys indicated the infestation was likely isolated near a public boat access. A 2.4-hectare areacontaining the known z
Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)
The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha, and quagga, D. bugensis, mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens, following non-la
Use of alternating and pulsed direct current electrified fields for zebra mussel control
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated
Effects of the biopesticide Zequanox® on reproduction and early development of the fathead minnow (Pimephales promelas)
The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems in the United States. Previous toxicity trials with nontarget organisms, including several young-of-the-year fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenid mussels, but data are lacking on the treatment-related effects on reproduction and early life stage development
Safety of the molluscicide Zequanox (R) to nontarget macroinvertebrates Gammarus lacustris (Amphipoda: Gammaridae) and Hexagenia spp. (Ephemeroptera: Ephemeridae)
Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A), that was developed to control dreissenid mussels. In 2014, Zequanox became the second product registered by the United States Environmental Protection Agency (USEPA) for use in open water environments as a molluscicide. Previous nontarget studies demonstrated the safety and selectivity of P. flu
Science and Products
- Science
Avoidance behavior of cold-, cool-, and warmwater fish exposed to Zequanox in a two-choice preference chamber
Zebra (Dreissenia polymorpha, Pallas 1771) and quagga (D. bugensis, Andrusov 1897) mussels, collectively referred to as dreissenid mussels, are invasive bivalves native to the Ponto-Caspian region of Eurasia (Stepien et al. 2013; Benson 2018a). High fecundity and a free-swimming planktonic life stage allow for easy and rapid dispersal of dreissenid mussels (Mackie 1991; Marsden et al. 2013)...Assessment of Open Water Zequanox Applications for Controlling Dreissenid Mussels within an Inland Lake
Invasion of dreissenid mussels (zebra and quagga mussels, Dreissena polymorpha and D. rostriformis bugensis, respectively) into the Great Lakes and Mississippi River Basins has resulted in estimated economic impacts as high as $1 billion annually for maintenance and repair of biofouled water conveyance systems and other infrastructures (Pimentel et al. 2005).Invasive Mussel Control Science: Management Tools for Assessing the Risks and Control of Invasive Dreissenid Species
Invasive zebra and quagga mussels (Dreissena polymorpha and D. rostriformis bugensis, respectively) are causing significant ecological and economic impacts and the scope of these impacts increases as they continue to spread across North America. The USGS conducts science to inform management actions for controlling and mitigating the impacts of invasive mussels. Studies include evaluation and...Use of electrified fields to control dreissenid mussels
Zebra and quagga mussels were first introduced into the Great Lakes in the 1980’s and they have since expanded to over 750 inland lakes in addition to the 5 Great Lakes (http://fl.biology.usgs.gov/Nonindigenous_Species/Zebra_mussel_distribution/zebra_mussel_distribution.html, accessed 8/5/2015). A 2009 study conducted by the Idaho Aquatic Nuisance Species Taskforce estimated the annual economic...Exposure-Related Effects of Zequanox on Lake Sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush) Survival and Condition
A dead-cell, spray-dried powder formulation of Pseudomonas fluorescens, strain CL145A was recently approved by the US Environmental Protection Agency (EPA) for control of dreissenid mussels (zebra mussel, Dreissena polymorpha and quagga mussel, Dreissena rostriformis bugensis) in open-water environments. The EPA approved product, Zequanox® (registration number 84059-15) is manufactured by Marrone...Temperature-dependent toxicity of molluscicides to zebra mussels
Zebra mussels (Dreissenia polymorpha) are native to the Black, Caspian, and Aral Seas of eastern Europe (Gollasch and Leppäkoski 1999) and they were likely introduced into Lake Erie as veliger larvae in the summer or fall of 1985 (Hebert et al. 1989). Their high reproductive capacity and planktonic larval stage enable zebra mussels to rapidly disperse (Birnbaum 2011). Less than 10 years after... - Data
Field Evaluation of an Improved Solid TFM Formulation for Use in Treating Small Tributary Streams, data
This study builds upon work conducted under the Great Lakes Fishery Commission (GLFC) Technical Assistance Program that was funded to prepare and evaluate an alternative solid formulation of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) in a laboratory flume. The project aligns within the lampricide theme in the Sea Lamprey Research Program by improving lampricide performance, efficacy, andDetermination of the seasonality effect on sea lamprey and TFM efficacy - Year One
Controlling larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries with of 4-Nitro-3-(trifluoromethyl)phenol (TFM, a lampricide) is essential to reducing the number of spawning-phase sea lamprey, an invasive species capable of collapsing Great Lakes fisheries. An important component of treating streams with lampricide is ensuring that the proper amount of TFM is applied, effectively coAvoidance behavior of cold-, cool-, and warmwater fish exposed to Zequanox in a two-choice preference chamber, data release
Zequanox® is an EPA-registered biopesticide for controlling populations of dreissenid mussels (zebra and quagga mussels). Zequanox has demonstrated selective toxicity toward dreissenid mussels; however, a recent laboratory study indicated the potential for Zequanox to impact a non-target salmonid species. We assessed the potential exposure risk by observing the avoidance behavior of two reAssessments of the binary mixtures of four toxicants on Zebra Mussel (Dreissena polymorpha) mortality, data release
This dataset captures the Zebra Mussel dose-response to four independent toxicants and each binary mixture of those toxicants. Toxicants included EarthTec QZ, Clam-Trol CT-2 (syn. Spectrus CT1300), niclosamide, and potassium chloride. Each dose of toxicant was verified with either ICP-OES or UHPLC, depending on analyte, and comparison to a standard curve. Mortality was tabulated for each observatiAssessment of uncontained Zequanox applications in a Midwestern lake data
We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets includedExposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush) Data
The environmental fate, persistence, and point-source discharge of traditional molluscicidal compounds led to the development of an alternative biomolluscicide, Zequanox. Previous studies evaluated the efficacy and non-target animal safety of Zequanox in laboratory, mesocosm, and field enclosure studies. One study indicated sensitivity of salmonid species and lake sturgeon (Acipenser fulvescens Ra - Publications
Filter Total Items: 25
Concentration addition and independent action assessments of the binary mixtures of four toxicants on zebra mussel (Dreissena polymorpha) mortality
Researchers most often focus on individual toxicants when identifying effective chemical control agents for aquatic invasive species; however, toxicant mixtures may elicit synergistic effects. Synergistic effects may decrease required concentrations and shorten exposure durations for treatments. We investigated four toxicants (EarthTec QZ, Clam-Trol CT-2, niclosamide, and potassium chloride) thatField evaluation of an improved solid TFM formulation for use in treating small tributary streams
A solid lampricide formulation containing 23% 3-trifluoromethyl-4-nitrophenol (TFM) as the active ingredient was developed in the mid-1980s for use in small tributaries of dendritic streams during routine treatments to kill larval sea lamprey. This TFM bar formulation was designed to use a matrix of commercially prepared surfactants that would dissolve and slowly release their TFM payload over anAvoidance of cold-, cool-, and warm-water fishes to Zequanox® exposure
Zequanox® is a biopesticide registered by the U.S. Environmental Protection Agency (USEPA) and the Canadian Pest Management Regulatory Agency for controlling dreissenid mussels with demonstrated selective toxicity. However, some research has indicated that Zequanox may impact the body condition and survival of some non-target species. We assessed avoidance behaviors of two species of cold-, cool-,Development and evaluation of an improved TFM formulation for use in feeder stream treatments
The binational Great Lakes Fishery Commission sponsored Sea Lamprey Control Program effectively utilizes a variety of lampricide tools to keep populations of parasitic sea lampreys in the Great Lakes at levels that do not cause undue economic or ecological damage. The most widely used toxicant used in lampricide formulations is 3-trifluoromethyl-4-nitrophenol (TFM). In typical treatments, a liquidAssessment of uncontained Zequanox applications for zebra mussel control in a Midwestern lake
Zebra mussels (Dreissena polymorpha) are invasive bivalves that have perturbed aquatic ecosystems within North America since their introduction in the mid-1980s. Control of zebra mussels has largely been restricted to raw water conveyance systems and associated infrastructures because few control products are registered for application in surface waters. The biopesticide Zequanox was registered inOptimum electrofishing waveforms and parameters to induce a capture-prone response in juvenile Grass Carp
Grass Carp (Ctenopharyngodon idella) are a non-native species to North America that were first introduced for vegetation control in the 1960s. However, wild-reproducing Grass Carp can negatively impact aquatic habitats and aquatic communities by consuming substantial amounts of aquatic vegetation and increasing turbidity. Numerous fisheries techniques have been used in an attempt to control or eEffects of temperature and exposure duration on four potential rapid-response tools for zebra mussel (Dreissena polymorpha) eradication
Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways by early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous erA bioassay assessment of a zebra mussel (Dreissena polymorpha) eradication treatment
Zebra mussels (Dreissena polymorpha, Pallas, 1771) are an aquatic invasive species in theUnited States, and new infestations of zebra mussels can rapidly expand into dense colonies. Zebramussels were first reported in Marion Lake, Dakota County, Minnesota, in September 2017, andsurveys indicated the infestation was likely isolated near a public boat access. A 2.4-hectare areacontaining the known zExposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)
The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha, and quagga, D. bugensis, mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens, following non-laUse of alternating and pulsed direct current electrified fields for zebra mussel control
Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluatedEffects of the biopesticide Zequanox® on reproduction and early development of the fathead minnow (Pimephales promelas)
The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems in the United States. Previous toxicity trials with nontarget organisms, including several young-of-the-year fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenid mussels, but data are lacking on the treatment-related effects on reproduction and early life stage developmentSafety of the molluscicide Zequanox (R) to nontarget macroinvertebrates Gammarus lacustris (Amphipoda: Gammaridae) and Hexagenia spp. (Ephemeroptera: Ephemeridae)
Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A), that was developed to control dreissenid mussels. In 2014, Zequanox became the second product registered by the United States Environmental Protection Agency (USEPA) for use in open water environments as a molluscicide. Previous nontarget studies demonstrated the safety and selectivity of P. flu