Jon Amberg, PhD
Jon Amberg is the Center Director for the Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin.
Science and Products
Filter Total Items: 14
Flocks of a feather dock together: Using Docker and HTCondor to link high-throughput computing across the USGS
USGS scientists often face computationally intensive tasks that require high-throughput computing capabilities. Several USGS facilities use HTCondor to run their computational pools but are not necessarily connected to the larger USGS pool. This project demonstrated how to connect HTCondor pools by flocking, or coordinating, within the USGS. In addition to flocking the Upper Midwest...
Increasing Efficiency and Throughput of eDNA
The results from this study will provide protocols for the collection, processing and analysis of environmental deoxyribonucleic acids (eDNA) samples for monitoring aquatic invasive species like Asian carp. These protocols have the potential to be used by fishery management agencies to monitor aquatic invasive species throughout the United States.
Use of antimycin-microparticles and corn feed to control Common Carp Cyprinus carpio
Attempts to control carp, Cyprinus carpio, populations in Minnesota (and elsewhere) date back to early 1900s (Moyle et al. 1950, Moyle and Kuehn 1964). Early efforts focused on removing large numbers of adult carp, often by targeting their winter aggregations using seine nets. Such was the strategy in Minnesota in 1930s and 40s, where carp were systematically removed with nets in dozens of lakes...
Correlating seasonal trends and occupancy of bigheaded carp eDNA to land use and stream characteristics
The results of this study will advance our understanding of environmental DNA (eDNA) and how eDNA signatures change as fish behavior changes over the course of the open water season. This will further develop eDNA as an easy sampling method to collect information about invading populations or cryptic species that are difficult to capture so that resource managers can be given better information to...
Relationship between fish presence and abundance in Arizona standing waters measured through standard techniques and environmental DNA
The unpredictability of DNA degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River, near...
Assessing the properties of RNA degradation in complex environmental water samples
The unpredictability of nucleic acid degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River...
Filter Total Items: 53
Tests in a semi-natural environment suggest that bait and switch strategy could be used to control invasive Common Carp Tests in a semi-natural environment suggest that bait and switch strategy could be used to control invasive Common Carp
Common Carp (Cyprinus carpio Linnaeus, 1758), is a highly invasive species that has had profound effects on biodiversity and ecosystem services. Many Carp management methods have been applied including physical removal, pesticide treatments of whole lakes, and water drawdowns. Herein, we tested key elements of a potential “bait and switch” approach in which corn could be used to induce...
Authors
Peter J Hundt, Jon Amberg, Blake W. Sauey, Kristen Vacura, Przemyslaw G. Bajer
Carbon dioxide-induced mortality of four species of North American fishes Carbon dioxide-induced mortality of four species of North American fishes
Fisheries managers have a growing interest in the use of carbon dioxide (CO2) as a tool for controlling invasive fishes. However, limited published data exist on susceptibility of many commonly encountered species to elevated CO2 concentrations. Our objective was to estimate the 24-h 50% lethal concentration (LC50) and 95% lethal concentration (LC95) of CO2 for four fishes (Rainbow Trout
Authors
Hilary B. Treanor, Andrew M. Ray, Jon Amberg, Mark P. Gaikowski, Jason E. Ilgen, Robert Gresswell, Leslie Gains-Germain, Molly A H Webb
A round-robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels A round-robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels
Resource managers may be hesitant to make decisions based on environmental (e)DNA results alone since eDNA is an indirect method of species detection. One way to reduce the uncertainty of eDNA is to identify laboratory‐based protocols that ensure repeatable and reproducible results. We conducted a double‐blind round‐robin analysis of probe‐based assays for DNA of dreissenid (Dreissena...
Authors
Adam J. Sepulveda, Patrick R. Hutchins, Craig Jackson, Carl O. Ostberg, Matthew Laramie, Jon Amberg, Timothy D. Counihan, Andrew B. Hoegh, David S. Pilliod
Adding invasive species bio-surveillance to the U.S. Geological Survey streamgage network Adding invasive species bio-surveillance to the U.S. Geological Survey streamgage network
The costs of invasive species in the United States alone are estimated to exceed US$100 billion per year so a critical tactic in minimizing the costs of invasive species is the development of effective, early-detection systems. To this end, we evaluated the efficacy of adding environmental (e)DNA surveillance to the U.S. Geological Survey (USGS) streamgage network, which consists of > 8...
Authors
Adam J. Sepulveda, Christian Schmidt, Jon Amberg, Patrick R. Hutchins, Christian Stratton, Christopher A. Mebane, Matthew Laramie, David S. Pilliod
Refinement of eDNA as an early monitoring tool at the landscape-level: Study design considerations Refinement of eDNA as an early monitoring tool at the landscape-level: Study design considerations
Natural resource managers use data on the spatial range of species to guide management decisions. These data come from survey or monitoring efforts that use a wide variety of tools. Environmental DNA (eDNA) is a surveillance tool that uses genetic markers for detecting species and holds potential as a tool for large-scale monitoring programs. Two challenges of eDNA-based studies are
Authors
Erica L. Mize, Richard A. Erickson, Christopher M. Merkes, N. Berndt, K.D. Bockrath, J. Credico, N. Grueneis, J. Merry, Kyle Mosel, M.T. Tuttle-Lau, K. Von Ruden, Jon Amberg, K. Baerwaldt, S.T. Finney, E.M. Monroe
Using environmental DNA to extend the window of early detection for dreissenid mussels Using environmental DNA to extend the window of early detection for dreissenid mussels
Tools that bolster early detection of invasive species are needed to prevent their spread. In this study, we assessed if environmental DNA (eDNA) can extend the seasonal window for dreissenid mussel early detection beyond that of plankton tows, which are limited to warmer seasons when mussel larvae are present. We focused eDNA sampling efforts at multiple sites in Tiber Reservoir...
Authors
Adam J. Sepulveda, Jon Amberg, Erik Hanson
Science and Products
Filter Total Items: 14
Flocks of a feather dock together: Using Docker and HTCondor to link high-throughput computing across the USGS
USGS scientists often face computationally intensive tasks that require high-throughput computing capabilities. Several USGS facilities use HTCondor to run their computational pools but are not necessarily connected to the larger USGS pool. This project demonstrated how to connect HTCondor pools by flocking, or coordinating, within the USGS. In addition to flocking the Upper Midwest...
Increasing Efficiency and Throughput of eDNA
The results from this study will provide protocols for the collection, processing and analysis of environmental deoxyribonucleic acids (eDNA) samples for monitoring aquatic invasive species like Asian carp. These protocols have the potential to be used by fishery management agencies to monitor aquatic invasive species throughout the United States.
Use of antimycin-microparticles and corn feed to control Common Carp Cyprinus carpio
Attempts to control carp, Cyprinus carpio, populations in Minnesota (and elsewhere) date back to early 1900s (Moyle et al. 1950, Moyle and Kuehn 1964). Early efforts focused on removing large numbers of adult carp, often by targeting their winter aggregations using seine nets. Such was the strategy in Minnesota in 1930s and 40s, where carp were systematically removed with nets in dozens of lakes...
Correlating seasonal trends and occupancy of bigheaded carp eDNA to land use and stream characteristics
The results of this study will advance our understanding of environmental DNA (eDNA) and how eDNA signatures change as fish behavior changes over the course of the open water season. This will further develop eDNA as an easy sampling method to collect information about invading populations or cryptic species that are difficult to capture so that resource managers can be given better information to...
Relationship between fish presence and abundance in Arizona standing waters measured through standard techniques and environmental DNA
The unpredictability of DNA degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River, near...
Assessing the properties of RNA degradation in complex environmental water samples
The unpredictability of nucleic acid degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River...
Filter Total Items: 53
Tests in a semi-natural environment suggest that bait and switch strategy could be used to control invasive Common Carp Tests in a semi-natural environment suggest that bait and switch strategy could be used to control invasive Common Carp
Common Carp (Cyprinus carpio Linnaeus, 1758), is a highly invasive species that has had profound effects on biodiversity and ecosystem services. Many Carp management methods have been applied including physical removal, pesticide treatments of whole lakes, and water drawdowns. Herein, we tested key elements of a potential “bait and switch” approach in which corn could be used to induce...
Authors
Peter J Hundt, Jon Amberg, Blake W. Sauey, Kristen Vacura, Przemyslaw G. Bajer
Carbon dioxide-induced mortality of four species of North American fishes Carbon dioxide-induced mortality of four species of North American fishes
Fisheries managers have a growing interest in the use of carbon dioxide (CO2) as a tool for controlling invasive fishes. However, limited published data exist on susceptibility of many commonly encountered species to elevated CO2 concentrations. Our objective was to estimate the 24-h 50% lethal concentration (LC50) and 95% lethal concentration (LC95) of CO2 for four fishes (Rainbow Trout
Authors
Hilary B. Treanor, Andrew M. Ray, Jon Amberg, Mark P. Gaikowski, Jason E. Ilgen, Robert Gresswell, Leslie Gains-Germain, Molly A H Webb
A round-robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels A round-robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels
Resource managers may be hesitant to make decisions based on environmental (e)DNA results alone since eDNA is an indirect method of species detection. One way to reduce the uncertainty of eDNA is to identify laboratory‐based protocols that ensure repeatable and reproducible results. We conducted a double‐blind round‐robin analysis of probe‐based assays for DNA of dreissenid (Dreissena...
Authors
Adam J. Sepulveda, Patrick R. Hutchins, Craig Jackson, Carl O. Ostberg, Matthew Laramie, Jon Amberg, Timothy D. Counihan, Andrew B. Hoegh, David S. Pilliod
Adding invasive species bio-surveillance to the U.S. Geological Survey streamgage network Adding invasive species bio-surveillance to the U.S. Geological Survey streamgage network
The costs of invasive species in the United States alone are estimated to exceed US$100 billion per year so a critical tactic in minimizing the costs of invasive species is the development of effective, early-detection systems. To this end, we evaluated the efficacy of adding environmental (e)DNA surveillance to the U.S. Geological Survey (USGS) streamgage network, which consists of > 8...
Authors
Adam J. Sepulveda, Christian Schmidt, Jon Amberg, Patrick R. Hutchins, Christian Stratton, Christopher A. Mebane, Matthew Laramie, David S. Pilliod
Refinement of eDNA as an early monitoring tool at the landscape-level: Study design considerations Refinement of eDNA as an early monitoring tool at the landscape-level: Study design considerations
Natural resource managers use data on the spatial range of species to guide management decisions. These data come from survey or monitoring efforts that use a wide variety of tools. Environmental DNA (eDNA) is a surveillance tool that uses genetic markers for detecting species and holds potential as a tool for large-scale monitoring programs. Two challenges of eDNA-based studies are
Authors
Erica L. Mize, Richard A. Erickson, Christopher M. Merkes, N. Berndt, K.D. Bockrath, J. Credico, N. Grueneis, J. Merry, Kyle Mosel, M.T. Tuttle-Lau, K. Von Ruden, Jon Amberg, K. Baerwaldt, S.T. Finney, E.M. Monroe
Using environmental DNA to extend the window of early detection for dreissenid mussels Using environmental DNA to extend the window of early detection for dreissenid mussels
Tools that bolster early detection of invasive species are needed to prevent their spread. In this study, we assessed if environmental DNA (eDNA) can extend the seasonal window for dreissenid mussel early detection beyond that of plankton tows, which are limited to warmer seasons when mussel larvae are present. We focused eDNA sampling efforts at multiple sites in Tiber Reservoir...
Authors
Adam J. Sepulveda, Jon Amberg, Erik Hanson