Nathan Johnson is a Research Biologist at the USGS Wetland and Aquatic Research Center.
EDUCATION
Ph.D., Fisheries and Aquatic Sciences, University of Florida
M.S., Fisheries and Wildlife Sciences, Virginia Tech University
B.S., Fisheries Science, Virginia Tech University
RESEARCH
Nathan Johnson's research focuses on investigating species boundaries, distributions, and conservation priorities of freshwater mussels in the Southeast. Raised in eastern Virginia, Nate received both his bachelor’s and master’s degrees from the Fisheries and Wildlife Department at Virginia Tech and earned his Ph.D. at the University of Florida. His undergraduate research focused on conservation genetics and propagation of endangered freshwater mussels and fishes. For his master’s, Nate looked at the genetics of disease resistance in rainbow trout at the USDA-National Center for Cool and Coldwater Aquaculture in Leetown, WV. By developing new molecular markers to trace pedigrees and populations structure, he identified associations between major histocompatibility genes and disease resistance. His dissertation research resolved long-standing uncertainties about the systematics of freshwater mussels that will aid in the development of more effective conservation management strategies. This includes documenting mussel diversity in rivers of the North American Coastal Plain using DNA barcoding and evaluating the processes responsible for their current geographic distribution (phylogeography). He is active in a variety of professional societies including the American Fisheries Society and the Freshwater Mollusk Conservation Society and currently partnered with the Florida Museum, the Smithsonian Institute, Florida Fish and Wildlife Conservation Commission, and the US Fish and Wildlife Service. Outside of professional interests, he enjoys traveling, mountain biking, fishing, and surfing.
Science and Products
Development and Implementation of Environmental DNA (eDNA) Tools to Aid Listing and Recovery Efforts for Imperiled and Common Freshwater Mussels
Using Molecular Tools to Recalibrate Freshwater Mussel Taxonomy with a Focus on Imperiled Species
Life History Characterization and Host Fish Identification for Federally Listed and Imperiled Freshwater Mussel Species in the Suwannee River Basin in Georgia and Florida
Evaluation of Stream Reaches for Mussel Reintroduction in the Upper Coosa Watershed, NW Georgia
Molecular, morphological, and distributional data supporting the recognition of an undescribed freshwater mussel endemic to the Edwards Plateau in the Colorado River basin
Molecular resources for investigating relationships among freshwater mussels (Bivalvia: Unionoida) with a focus on the genus Alasmidonta
Red Lionfish DNA data collected from Florida, USA and around the invasive distribution from 2007 to 2016
Novel genetic resources for Clubshell freshwater mussels (Pleurobema clava, P. oviforme) for enhanced conservation
Molecular data to investigate phylogeographic patterns, species boundaries, and demographic history of a North American freshwater mussel species complex (Bivalvia: Unionidae)
Novel genetic resources to facilitate future molecular studies in freshwater mussels (Bivalvia: Unionidae)
Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli
Anchored hybrid enrichment in Potamilus mussels
Molecular, morphometric, and distribution information for the freshwater mussel genus Potamilus and newly described species, Potamilus streckersoni sp. nov.
Salinity tolerance among three freshwater mussels (Bivalvia: Unionidae) from Gulf Coastal Plain drainages
Molecular and morphological data on two species complexes in the freshwater mussel genus Cyclonaias
A novel assembly pipeline and functional annotations for targeted sequencing: A case study on the globally threatened Margaritiferidae (Bivalvia: Unionida)
Hidden in the hills: Phylogeny of the freshwater mussel genus Alasmidonta (Bivalvia: Unionidae) and description of a new species
Genetic and morphological characterization of the freshwater mussel clubshell species complex (Pleurobema clava and Pleurobema oviforme) to inform conservation planning
Biogeography of freshwater mussels (Bivalvia: Unionida) in Texas and implications on conservation biology
Using a multi-model ensemble approach to determine biodiversity hotspots with limited occurrence data in understudied areas: An example using freshwater mussels in México
Genetic analysis of red lionfish Pterois volitans from Florida, USA, leads to alternative North Atlantic introduction scenarios
Establishing conservation units to promote recovery of two threatened freshwater mussel species (Bivalvia: Unionida: Potamilus)
Rediscovery and genetic confirmation of the Threeridge Mussel, Amblema plicata (Say, 1817) (Bivalvia, Unionidae), in the Choctawhatchee River, Florida, USA
Gulf Coast vicariance shapes phylogeographic history of a North American freshwater mussel species complex
Resolving species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli (Bivalvia: Unionidae)
Novel molecular resources to facilitate future genetics research on freshwater mussels (Bivalvia: Unionidae)
The utility of zooarchaeological data to guide listing efforts for an imperiled mussel species (Bivalvia: Unionidae: Pleurobema riddellii)
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
- Science
Development and Implementation of Environmental DNA (eDNA) Tools to Aid Listing and Recovery Efforts for Imperiled and Common Freshwater Mussels
Researchers will develop and optimize an eDNA assay to delineate the current distribution of P. inflatus. The assay will then be used to provide up-to-date distributional information and detection rates for P. inflatus in the Pearl River basin.Using Molecular Tools to Recalibrate Freshwater Mussel Taxonomy with a Focus on Imperiled Species
Freshwater mussels of the family Unionidae, also known as naiads, pearly mussels, freshwater clams, or unionids, are a diverse group of bivalve mollusks that are distributed on every continent except Antarctica. Approximately 300 species are known from the United States, with most of this diversity residing in rivers of the Southeast where many endemic taxa have evolved.Life History Characterization and Host Fish Identification for Federally Listed and Imperiled Freshwater Mussel Species in the Suwannee River Basin in Georgia and Florida
Freshwater mussels are considered the most imperiled group of animals in the United States. These animals provide valuable ecological services by filtering water, sequestering nutrients, and providing forage for migratory birds, small mammals, and turtles. They also have a unique and complex life cycle that makes them especially vulnerable to human disturbances. It includes a parasitic larval...Evaluation of Stream Reaches for Mussel Reintroduction in the Upper Coosa Watershed, NW Georgia
The Conasauga River in northwest Georgia and southeast Tennessee harbors the majority of mussel diversity still found in the Georgia portion of the Upper Coosa Basin. While the Conasauga historically supported at least 44 mussel species, only about 20 species remain. - Data
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Molecular, morphological, and distributional data supporting the recognition of an undescribed freshwater mussel endemic to the Edwards Plateau in the Colorado River basin
The widespread distribution of Strophitus undulatus, as well as high intraspecific morphological variation, has led previous authors to doubt the taxon is representative of a single species. Here we provide detailed information for all the freshwater mussels and associated DNA sequence data utilized to test species boundaries in S. undulatus. These molecular data, in combination with morphologicalMolecular resources for investigating relationships among freshwater mussels (Bivalvia: Unionoida) with a focus on the genus Alasmidonta
Longstanding taxonomic uncertainties have limited conservation efforts for species currently assigned to the freshwater mussel genus Alasmidonta. Here, we present mitochondrial and nuclear DNA sequence data needed to assess the genus- and species-level taxonomy of Alasmidonta. These molecular data allowed us to test whether cryptic diversity exists within Alasmidonta and whether A. triangulata andRed Lionfish DNA data collected from Florida, USA and around the invasive distribution from 2007 to 2016
Red lionfish (Pterois volitans) have become a successful invasive predator across the Northwestern Atlantic, Caribbean, and Gulf of Mexico (GoM). Previous investigations have identified the southeast coast of Florida as the original site of introduction, but no region-wide genetic study has directly addressed the question of introduction location(s). This dataset includes previously unpublished reNovel genetic resources for Clubshell freshwater mussels (Pleurobema clava, P. oviforme) for enhanced conservation
Here we provide public access to two mitochondrial DNA sequence alignments, multi-locus microsatellite DNA genotypes, morphometric measurements and details on all specimens utilized in Morrison et al., 2021 (In press).Molecular data to investigate phylogeographic patterns, species boundaries, and demographic history of a North American freshwater mussel species complex (Bivalvia: Unionidae)
Here we provide public access to three DNA sequence alignments (COI, ND1, ITS1) and details on all specimens utilized in Keogh et al. (in review).Novel genetic resources to facilitate future molecular studies in freshwater mussels (Bivalvia: Unionidae)
Here we provide public access to six DNA sequence alignments and details on all specimens utilized in Smith and Johnson (2020).Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum heighAnchored hybrid enrichment in Potamilus mussels
Freshwater mussels are a species-rich group with biodiversity patterns shaped by life history strategies that include an obligate parasitic larval stage. Anchored hybrid enrichment (AHE) and ancestral character reconstruction (ACR) revealed convergence of life history strategies involving larval morphologies that reduce size to increase fecundity and require growth during encapsulation to increaseMolecular, morphometric, and distribution information for the freshwater mussel genus Potamilus and newly described species, Potamilus streckersoni sp. nov.
Inaccurate systematics confound our ability to determine evolutionary processes that have led to the diversification of many taxa. The North American freshwater mussel tribe Lampsilini, Ihering, 1901, is one of the more well-studied groups in Unionidae; however, many supraspecific relationships between lampsiline genera remain unresolved. Two genera that have been largely overlooked are Leptodea aSalinity tolerance among three freshwater mussels (Bivalvia: Unionidae) from Gulf Coastal Plain drainages
An important physiological constraint influencing distributions of coastal freshwater organisms is their tolerance for saline conditions. We experimentally evaluated salinity tolerance for three freshwater mussel species (Utterbackia imbecillis, Elliptio jayensis, and Glebula rotundata). Mussels were transferred abruptly from well water to one of five treatments (0 [control], 6, 12, 18 or 24 partsMolecular and morphological data on two species complexes in the freshwater mussel genus Cyclonaias
We examined species boundaries within an imperiled group of freshwater mussels in the genus Cyclonaias (Bivalvia: Unionidae) using morphometrics, molecular phylogenetics, and multispecies coalescent models to help guide pending conservation assessments and legislative decisions. Congruence across all lines of evidence indicated that current taxonomy overestimates diversity in the C. pustulosa spec - Publications
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A novel assembly pipeline and functional annotations for targeted sequencing: A case study on the globally threatened Margaritiferidae (Bivalvia: Unionida)
The proliferation of genomic sequencing approaches has significantly impacted the field of phylogenetics. Target capture approaches provide a cost-effective, fast and easily applied strategy for phylogenetic inference of non-model organisms. However, several existing target capture processing pipelines are incapable of incorporating whole genome sequencing (WGS). Here, we develop a new pipeline foAuthorsAndré Gomes-dos-Santos, Elsa Froufe, John M. Pfeiffer, Nathan Johnson, Chase H. Smith, André M. Machado, L. Filipe C. Castro, Van Tu Do, Akimasa Hattori, Nicole Garrison, Nathan V. Whelan, Ivan N. Bolotov, Ilya V. Vikhrev, Alexander V. Kondakov, Mohamed Ghamizi, Vincent Prié, Arthur E. Bogan, Manuel Lopes LimaHidden in the hills: Phylogeny of the freshwater mussel genus Alasmidonta (Bivalvia: Unionidae) and description of a new species
Inaccurate taxonomy can lead to species in need of conservation being overlooked, which makes revisionary systematics crucially important for imperilled groups. The freshwater mussel genus Alasmidonta is one such group in need of study. Here, we take a multilocus phylogenetic approach to assess species-level taxonomy of Alasmidonta and test monophyly of this genus. Phylogenetic inference resultedAuthorsNathan V. Whelan, Nathan Johnson, Ashantye' S. Williams, Michael A. Perkins, Caitlin E. Beaver, Jason W. MaysGenetic and morphological characterization of the freshwater mussel clubshell species complex (Pleurobema clava and Pleurobema oviforme) to inform conservation planning
The shell morphologies of the freshwater mussel species Pleurobema clava (federally endangered) and Pleurobema oviforme (species of concern) are similar, causing considerable taxonomic confusion between the two species over the last 100 years. While P. clava was historically widespread throughout the Ohio River basin and tributaries to the lower Laurentian Great Lakes, P. oviforme was confined toAuthorsCheryl Morrison, Nathan Johnson, Jess W Jones, Michael Eackles, Aaron Aunins, Daniel Bruce Fitzgerald, Eric M. Hallerman, Timothy L. KingBiogeography of freshwater mussels (Bivalvia: Unionida) in Texas and implications on conservation biology
AimBiogeography seeks to identify and explain the spatial distributions of species and has become an important tool used by conservationists to protect and manage aquatic organisms. Texas, located in the southwestern United States, is home to 52 species of freshwater mussels, 9 of which are endemic to Texas and 7 that are endemic to Texas and neighboring states or countries. There have been two maAuthorsMichael de Moulpied, Chase H. Smith, Clint R. Robertson, Nathan Johnson, Roel Lopez, Charles R. RandklevUsing a multi-model ensemble approach to determine biodiversity hotspots with limited occurrence data in understudied areas: An example using freshwater mussels in México
Species distribution models (SDMs) are an increasingly important tool for conservation particularly for difficult-to-study locations and with understudied fauna. Our aims were to (1) use SDMs and ensemble SDMs to predict the distribution of freshwater mussels in the Pánuco River Basin in Central México; (2) determine habitat factors shaping freshwater mussel occurrence; and (3) use predicted occupAuthorsAlexander H. Kiser, Kevin S. Cummings, Jeremy S. Tiemann, Chase H. Smith, Nathan Johnson, Roel R. Lopez, Charles R. RandklevGenetic analysis of red lionfish Pterois volitans from Florida, USA, leads to alternative North Atlantic introduction scenarios
The red lionfish Pterois volitans is a successful invasive predator across the western North Atlantic, Caribbean, and Gulf of Mexico. The southeast coast of Florida (USA) has been identified as the original introduction location, but genetic analyses including Florida lionfish have yet to investigate introduction scenarios. Here, we assessed the potential lionfish invasion pathways using 1795 sequAuthorsMargaret Hunter, Caitlin Beaver, Nathan Johnson, Eleanor K. Bors, Antonio A. Mignucci-Giannoni, Brian R. Silliman, Dayne Buddo, Linda Searle, Edgardo Díaz-FergusonEstablishing conservation units to promote recovery of two threatened freshwater mussel species (Bivalvia: Unionida: Potamilus)
Population genomics has significantly increased our ability to make inferences about microevolutionary processes and demographic histories, which have the potential to improve protection and recovery of imperiled species. Freshwater mussels (Bivalvia: Unionida) represent one of the most imperiled groups of organisms globally. Despite systemic decline of mussel abundance and diversity, studies evalAuthorsChase H. Smith, Nathan Johnson, Clinton R. Robertson, Robert D. Doyle, Charles R. RandklevRediscovery and genetic confirmation of the Threeridge Mussel, Amblema plicata (Say, 1817) (Bivalvia, Unionidae), in the Choctawhatchee River, Florida, USA
Recent freshwater mussel research has resulted in rediscovery of several species presumed extinct. We report the rediscovery of Amblema plicata (Say, 1817) in 2019 from the Choctawhatchee River, Florida, USA. Amblema plicata has not been reported in the Choctawhatchee river basin since 1958, more than 61 years ago. This species was collected during the long-term monitoring of freshwater mussels inAuthorsLauren N. Patterson, Susan R. Geda, Nathan JohnsonGulf Coast vicariance shapes phylogeographic history of a North American freshwater mussel species complex
AimFreshwater mussels share habitat and are parasites of freshwater fishes during the larval life stage. Therefore, models of fish biogeography may also explain the historical biogeography of freshwater mussels. We tested this assumption using predictions of three biogeographic models constructed for northern Gulf of Mexico drainages on a freshwater mussel species complex. Specifically, we testedAuthorsSean M. Keogh, Nathan Johnson, James D. Williams, Charles R. Randklev, Andrew SimonsResolving species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli (Bivalvia: Unionidae)
Species are a fundamental unit of biology, and defining accurate species boundaries is integral to effective conservation and management of imperiled taxa. Freshwater mussels (Bivalvia: Unionidae) are among the most imperiled groups of organisms in North America, yet species boundaries remain uncertain for many taxa. The False Spike, Fusconaia mitchelli (Simpson in Dall, 1895), is a freshwater musAuthorsChase H. Smith, Nathan Johnson, Kaitlyn Havlik, Robert D. Doyle, Charles R. RandklevNovel molecular resources to facilitate future genetics research on freshwater mussels (Bivalvia: Unionidae)
Molecular data have been an integral tool in the resolution of the evolutionary relationships and systematics of freshwater mussels, despite the limited number of nuclear markers available for Sanger sequencing. To facilitate future studies, we evaluated the phylogenetic informativeness of loci from the recently published anchored hybrid enrichment (AHE) probe set Unioverse and developed novel SanAuthorsNathan Johnson, Chase H. SmithThe utility of zooarchaeological data to guide listing efforts for an imperiled mussel species (Bivalvia: Unionidae: Pleurobema riddellii)
The status of species in freshwater systems shift over time due to natural and anthropogenic causes. Determining the magnitude and cause of these shifts requires a long-term perspective. This process is complicated when there are also questions about the taxonomic validity of a species. Addressing these issues is important because both can undermine conservation and management efforts if incorrectAuthorsCharles R. Randklev, Steve Wolverton, Nathan Johnson, Chase H. Smith, Traci DuBose, Clint Robertson, Julian ConleyNon-USGS Publications**
Grobler, P. J., Jones, J.W., Johnson, N.A., Neves, R.J., and E.M. Hallerman. 2011. Homogeneity at Nuclear Microsatellite Loci Masks Mitochondrial Haplotype Diversity in the Endangered Fanshell Pearlymussel (Cyprogenia stegaria). Journal of Heredity 102(2): 196-206. [Link]**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
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