Rassa Draugelis-Dale, Ph.D. (Former Employee)
Science and Products
A Website Tool for Predicting Triploidy in Larval Fish Spawns
Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006)
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among sub-basins within LMNRA. To investigate whether environmental contaminant exposure is associated with alterations in male common carp...
Filter Total Items: 18
Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas
The Buffalo National River (BNR), on karst terrain in Arkansas, is considered an extraordinary water resource. Water collected in Spring 2017 along BNR was metagenomically analyzed using 16S rDNA, and for 17 months (5/2017–11/2018), bacterial responses were measured in relation to nutrients sampled along a stretch of BNR near a concentrated animal feed operation (CAFO) on Big Creek...
Authors
Jill Jenkins, Rassa Dale, Nina M. Hoffpauir, Brooke A Baudoin, Caroline Matkin, Lucas Driver, Shawn W Hodges, Bonnie L. Brown
Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin
Neonicotinoids (NEO) represent the main class of insecticides currently in use, with thiamethoxam (THX) and clothianidin (CLO) primarily applied agriculturally. With few comprehensive studies having been performed with non-target amphibians, the aim was to investigate potential biomarker responses along an adverse outcome pathway of NEO exposure, whereby data were collected on multiple...
Authors
Jill Jenkins, Katherine R. Hartop, Ghadeer Bukhari, Debra E. Howton, Kelly L. Smalling, Scott Mize, Michelle Hladik, Darren Johnson, Rassa Dale, Bonnie L. Brown
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp...
Authors
Jill A. Jenkins, Michael R. Rosen, Rassa O. Dale, Kathy R. Echols, Leticia Torres, Carla M. Wieser, Constance A. Kersten, S. Goodbred
An accurate method for measuring triploidy of larval fish spawns
A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to select treatments for efficient triploid...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Robert Glennon, Anita M. Kelly, Bonnie L. Brown, John Morrison
Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)
Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA
Persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (p,p′-DDE), and polybrominated diphenyl ethers (PBDEs) are stable, bioaccumulative, and widely found in the environment, wildlife, and the human population. To explore the hypothesis that reproduction in male fish is associated with environmental exposures in the lower Columbia...
Authors
Jill A. Jenkins, H.M. Olivier, R. O. Draugelis-Dale, B.E. Eilts, L. Torres, R. Patiño, Elena B. Nilsen, Steven L. Goodbred
Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay
Reduced recruitment of yellow perch has been noted for a number of years in certain urbanized watersheds (South and Severn Rivers) of the Chesapeake Bay. Other rapidly developing watersheds such as Mattawoman Creek are more recently showing evidence of reduced recruitment of anadromous fishes. In this study, we used a battery of biomarkers to better document the reproductive health of...
Authors
Vicki Blazer, Alfred E. Pinkney, Jill A. Jenkins, Luke R. Iwanowicz, Steven Minkkinen, Rassa O. Draugelis-Dale, James H. Uphoff
Sperm quality assessments for endangered razorback suckers Xyrauchen texanus
Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckers Xyrauchen texanus collected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167–343 mOsm/kg). We hypothesized that sperm quality of milt collected into...
Authors
Jill A. Jenkins, Bruce E. Eilts, Amy M. Guitreau, Chester R. Figiel, Rassa O. Draugelis-Dale, Terrence R. Tiersch
Davis Pond freshwater diversion biomonitoring: Prediversion and postdiversion freshwater fish data
No abstract available.
Authors
Jill A. Jenkins, Heather M. Olivier, Rassa Draugelis-Dale, Michael D. Kaller
Effects of herbivory and flooding on reforestation of baldcypress (Taxodium distichum [L.]) saplings planted in Caddo Lake, Texas
The effects of herbivory and flooding were examined on survival and growth of planted baldcypress (Taxodium distichum (L.) Rich.) saplings at three sites in Caddo Lake, TX, over a 4-yr period. There were two flood regimes (shallow periodic and deep continuous), where half of the saplings in each flood regime were protected by tree shelters to prevent herbivory. By the end of the first...
Authors
Bobby D. Keeland, Rassa O. Dale, Roy Darville, John W. McCoy
Tree growth and mortality during 20 years of managing a Green-Tree Reservoir in Arkansas, USA
Green-Tree Reservoirs (GTR) are bottomland hardwood forests that are flooded during late fall and winter to provide waterfowl habitat. Early reports suggested that increased moisture improved tree growth and mast production; however, recent reports showed reduced vigor and growth. This study examines the effects of 20 years of GTR management practices in the Felsenthal National Wildlife...
Authors
Bobby D. Keeland, Rassa O. Draugelis-Dale, John W. McCoy
A comparison of litter production in young and old baldcypress (Taxodium distichum L.) stands at Caddo Lake, Texas
Aboveground primary productivity for cypress forests was assessed from measurements of litter production in two age groups and in two hydrological regimes (standing water and free-flowing). Caddo Lake, located in northeast Texas on the Texas-Louisiana border, offered a unique study site since it is dominated by extensive stands composed entirely of Taxodium distichum (L.) Rich,...
Authors
John W. McCoy, Rassa O. Draugelis-Dale, Bobby D. Keeland, Roy Darville
Science and Products
A Website Tool for Predicting Triploidy in Larval Fish Spawns
Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006)
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among sub-basins within LMNRA. To investigate whether environmental contaminant exposure is associated with alterations in male common carp...
Filter Total Items: 18
Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas
The Buffalo National River (BNR), on karst terrain in Arkansas, is considered an extraordinary water resource. Water collected in Spring 2017 along BNR was metagenomically analyzed using 16S rDNA, and for 17 months (5/2017–11/2018), bacterial responses were measured in relation to nutrients sampled along a stretch of BNR near a concentrated animal feed operation (CAFO) on Big Creek...
Authors
Jill Jenkins, Rassa Dale, Nina M. Hoffpauir, Brooke A Baudoin, Caroline Matkin, Lucas Driver, Shawn W Hodges, Bonnie L. Brown
Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin
Neonicotinoids (NEO) represent the main class of insecticides currently in use, with thiamethoxam (THX) and clothianidin (CLO) primarily applied agriculturally. With few comprehensive studies having been performed with non-target amphibians, the aim was to investigate potential biomarker responses along an adverse outcome pathway of NEO exposure, whereby data were collected on multiple...
Authors
Jill Jenkins, Katherine R. Hartop, Ghadeer Bukhari, Debra E. Howton, Kelly L. Smalling, Scott Mize, Michelle Hladik, Darren Johnson, Rassa Dale, Bonnie L. Brown
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp...
Authors
Jill A. Jenkins, Michael R. Rosen, Rassa O. Dale, Kathy R. Echols, Leticia Torres, Carla M. Wieser, Constance A. Kersten, S. Goodbred
An accurate method for measuring triploidy of larval fish spawns
A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to select treatments for efficient triploid...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Robert Glennon, Anita M. Kelly, Bonnie L. Brown, John Morrison
Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)
Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA
Persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (p,p′-DDE), and polybrominated diphenyl ethers (PBDEs) are stable, bioaccumulative, and widely found in the environment, wildlife, and the human population. To explore the hypothesis that reproduction in male fish is associated with environmental exposures in the lower Columbia...
Authors
Jill A. Jenkins, H.M. Olivier, R. O. Draugelis-Dale, B.E. Eilts, L. Torres, R. Patiño, Elena B. Nilsen, Steven L. Goodbred
Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay
Reduced recruitment of yellow perch has been noted for a number of years in certain urbanized watersheds (South and Severn Rivers) of the Chesapeake Bay. Other rapidly developing watersheds such as Mattawoman Creek are more recently showing evidence of reduced recruitment of anadromous fishes. In this study, we used a battery of biomarkers to better document the reproductive health of...
Authors
Vicki Blazer, Alfred E. Pinkney, Jill A. Jenkins, Luke R. Iwanowicz, Steven Minkkinen, Rassa O. Draugelis-Dale, James H. Uphoff
Sperm quality assessments for endangered razorback suckers Xyrauchen texanus
Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckers Xyrauchen texanus collected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167–343 mOsm/kg). We hypothesized that sperm quality of milt collected into...
Authors
Jill A. Jenkins, Bruce E. Eilts, Amy M. Guitreau, Chester R. Figiel, Rassa O. Draugelis-Dale, Terrence R. Tiersch
Davis Pond freshwater diversion biomonitoring: Prediversion and postdiversion freshwater fish data
No abstract available.
Authors
Jill A. Jenkins, Heather M. Olivier, Rassa Draugelis-Dale, Michael D. Kaller
Effects of herbivory and flooding on reforestation of baldcypress (Taxodium distichum [L.]) saplings planted in Caddo Lake, Texas
The effects of herbivory and flooding were examined on survival and growth of planted baldcypress (Taxodium distichum (L.) Rich.) saplings at three sites in Caddo Lake, TX, over a 4-yr period. There were two flood regimes (shallow periodic and deep continuous), where half of the saplings in each flood regime were protected by tree shelters to prevent herbivory. By the end of the first...
Authors
Bobby D. Keeland, Rassa O. Dale, Roy Darville, John W. McCoy
Tree growth and mortality during 20 years of managing a Green-Tree Reservoir in Arkansas, USA
Green-Tree Reservoirs (GTR) are bottomland hardwood forests that are flooded during late fall and winter to provide waterfowl habitat. Early reports suggested that increased moisture improved tree growth and mast production; however, recent reports showed reduced vigor and growth. This study examines the effects of 20 years of GTR management practices in the Felsenthal National Wildlife...
Authors
Bobby D. Keeland, Rassa O. Draugelis-Dale, John W. McCoy
A comparison of litter production in young and old baldcypress (Taxodium distichum L.) stands at Caddo Lake, Texas
Aboveground primary productivity for cypress forests was assessed from measurements of litter production in two age groups and in two hydrological regimes (standing water and free-flowing). Caddo Lake, located in northeast Texas on the Texas-Louisiana border, offered a unique study site since it is dominated by extensive stands composed entirely of Taxodium distichum (L.) Rich,...
Authors
John W. McCoy, Rassa O. Draugelis-Dale, Bobby D. Keeland, Roy Darville