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Tonie Rocke

Tonie Rocke is a Research Epidemiologist at the National Wildlife Health Center.

Science and Products

A small brown bat sits on the side of a tree

Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
Learn More
Gloved hands holding a bat white glove scientists administers vaccine orally.

White-nose Syndrome Vaccine Updates

Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
White-nose Syndrome Vaccine Updates

White-nose Syndrome Vaccine Updates

Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
Learn More
Cluster of bats on a cave wall.

Field trials for testing of white-nose syndrome vaccine candidates

White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Field trials for testing of white-nose syndrome vaccine candidates

Field trials for testing of white-nose syndrome vaccine candidates

White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
Learn More
Image: Black-Footed Ferret

Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela  lutreola and  Neovison  vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela  lutreola and  Neovison  vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
Learn More
Little brown bat (Myotis lucifugus) in the Shipp Room, Hellhole cave

Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
By
Biological Threats and Invasive Species Research Program, Species Management Research Program, National Wildlife Health Center, Upper Midwest Environmental Sciences Center
Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
Learn More
Image: Plague Vaccine Bait

Vaccines

The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Vaccines

Vaccines

The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
Learn More
Prairie dog and SPV bait

Sylvatic Plague

Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Sylvatic Plague

Sylvatic Plague

Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
Learn More
Image: Bat with White-nose Syndrome

White-Nose Syndrome

White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
White-Nose Syndrome

White-Nose Syndrome

White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
Learn More
Mosquito, species not verified

Vector-Borne Diseases

Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Vector-Borne Diseases

Vector-Borne Diseases

Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
Learn More
Filter Total Items: 16
No Result Found
No results found.
Filter Total Items: 135

Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction

We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently...
Authors
P. Nol, J.L. Williamson, Tonie E. Rocke, Thomas M. Yuill
By
Ecosystems Mission Area, National Wildlife Health Center, Salton Sea

Recombinant raccoon pox vaccine protects mice against lethal plague Recombinant raccoon pox vaccine protects mice against lethal plague

Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the...
Authors
J.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, Tonie E. Rocke, D.T. Stinchcomb
By
Ecosystems Mission Area, National Wildlife Health Center

Determination of the median toxic dose of type C botulism in lactating dairy cows Determination of the median toxic dose of type C botulism in lactating dairy cows

Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose...
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, F.D. Galey, J.S. Cullor, A.A. Ardans
By
Ecosystems Mission Area, National Wildlife Health Center

New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos

An American white pelican (P. erythrorhyncos) was captured in August 1999 in California, USA. The bird was unable to fly away or evade the boat, and showed considerable aggression when captured. Numerous multifocal, 1 mm diameter, yellow masses were observed throughout the lungs and air sacs. The liver was yellow. A 6 cm diameter, firm spherical mass was present in the abdominal cavity...
Authors
P. Nol, R.E. Brannian, B.M. Berlowski, M. J. Wolcott, Tonie E. Rocke
By
Ecosystems Mission Area, National Wildlife Health Center

Failure to transmit avian vacuolar myelinopathy to mallard ducks Failure to transmit avian vacuolar myelinopathy to mallard ducks

Avian vacuolar myelinopathy (AVM) is a neurologic disease that has been diagnosed in free-ranging birds in the southeastern United States. Bald eagles (Haliaeetus leuocephalus), American coots (Fulica americana), and mallards (Anas platyrhynchos) have been affected. Previous investigations have not determined the etiology of this disease. In November and December 2002, we attempted to...
Authors
R. S. Larsen, Felicia B. Nutter, Tom Augspurger, Tonie E. Rocke, Nancy J. Thomas, Michael K. Stoskopf
By
Ecosystems Mission Area, National Wildlife Health Center

Vacuolar myelinopathy in waterfowl from a North Carolina impoundment Vacuolar myelinopathy in waterfowl from a North Carolina impoundment

Vacuolar myelinopathy was confirmed by light and electron microscopic examination of mallards (Anas platyrhynchos), ring-necked ducks (Aythya collaris), and buffleheads (Bucephala albeola) collected during an epizootic at Lake Surf in central North Carolina (USA) between November 1998 and February 1999. Clinical signs of affected birds were consistent with central nervous system...
Authors
T. Augspurger, John R. Fischer, Nancy Thomas, L. Sileo, Roger E. Brannian, Kimberli Miller, Tonie E. Rocke
By
Ecosystems Mission Area, National Wildlife Health Center

A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1 A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1

A serotype-specific polymerase chain reaction (PCR) assay was developed for detection and identification of Pasteurella multocida serotype 1, the causative agent of avian cholera in wild waterfowl. Arbitrarily primed PCR was used to detect DNA fragments that distinguish serotype 1 from the other 15 serotypes of P. multocida (with the exception of serotype 14). Oligonucleotide primers...
Authors
Tonie E. Rocke, S.R. Smith, A. Miyamoto, D.J. Shadduck
By
Ecosystems Mission Area, National Wildlife Health Center

Wildlife disease in the Colorado Delta as an indicator of ecosystem health Wildlife disease in the Colorado Delta as an indicator of ecosystem health

Ecosystem health and management are relatively new paradigms for environmental management (Costanza et al., 1992) and will be continually redefined and reevaluated relative to their purpose and utility in serving human society (Costanza et al., 1992; Sutter, 1993; Grumbine, 1994; Lackey, 1998, 1999). The stimulus for ecosystem management is often associated with sustaining or restoring...
Authors
Tonie E. Rocke, M. Friend
By
Ecosystems Mission Area, National Wildlife Health Center

PCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in PCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in

Botulism is one of the most important bird diseases world-wide and is caused by the intoxication with Botulinum-Neurotoxin-C1 (BoNt-C1), which is produced by toxigenic clostridia under appropriate conditions. Avian botulism leads regularly to large losses among the migrating bird populations breeding and resting at the saltwater pools of the Austrian national park Neusiedler See...
Authors
T.C. Zechmeister, A.H. Farnleitner, Tonie E. Rocke, F. Pittner, R. Rosengarten, R.L. Mach, A. Herzig, A.K.T. Kirschner
By
Ecosystems Mission Area, National Wildlife Health Center

Possible importance of algal toxins in the Salton Sea, California Possible importance of algal toxins in the Salton Sea, California

In response to wildlife mortality including unexplained eared grebe (Podiceps nigricollis) die-off events in 1992 and 1994 and other mortality events including large fish kills, a survey was conducted for the presence of algal toxins in the Salton Sea. Goals of this survey were to determine if and when algal toxins are present in the Salton Sea and to describe the phytoplankton...
Authors
Kristen M. Reifel, M. P. McCoy, Tonie E. Rocke, M. A. Tiffany, S. H. Hurlbert, D. J. Faulkner
By
Ecosystems Mission Area, National Wildlife Health Center, Salton Sea

Joining forces to improve our world Joining forces to improve our world

No abstract available.
Authors
W.B. Karesh, S.A. Osofsky, Tonie E. Rocke, P.L. Barrows
By
Ecosystems Mission Area, National Wildlife Health Center

A baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs A baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs

Laboratory and field studies were conducted between July and October 1999 to identify bait preference, biomarker efficacy, and bait acceptance rates for delivering an oral plague vaccine to black-tailed prairie dogs (Cynomys ludovicianus). Twenty juvenile captive prairie dogs were offered alfalfa baits containing either alfalfa, alfalfa and 5% molasses, or alfalfa, 5% molasses and 4%...
Authors
Terry E. Creekmore, Tonie E. Rocke, J. Hurley
By
Ecosystems Mission Area, National Wildlife Health Center

Science and Products

A small brown bat sits on the side of a tree

Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination

Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
Learn More
Gloved hands holding a bat white glove scientists administers vaccine orally.

White-nose Syndrome Vaccine Updates

Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
White-nose Syndrome Vaccine Updates

White-nose Syndrome Vaccine Updates

Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
Learn More
Cluster of bats on a cave wall.

Field trials for testing of white-nose syndrome vaccine candidates

White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Field trials for testing of white-nose syndrome vaccine candidates

Field trials for testing of white-nose syndrome vaccine candidates

White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
Learn More
Image: Black-Footed Ferret

Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela  lutreola and  Neovison  vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

Development of SARS-CoV-2 vaccine to support black-footed ferret conservation

The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela  lutreola and  Neovison  vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
Learn More
Little brown bat (Myotis lucifugus) in the Shipp Room, Hellhole cave

Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
By
Biological Threats and Invasive Species Research Program, Species Management Research Program, National Wildlife Health Center, Upper Midwest Environmental Sciences Center
Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics

Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
Learn More
Image: Plague Vaccine Bait

Vaccines

The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Vaccines

Vaccines

The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
Learn More
Prairie dog and SPV bait

Sylvatic Plague

Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Sylvatic Plague

Sylvatic Plague

Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
Learn More
Image: Bat with White-nose Syndrome

White-Nose Syndrome

White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
White-Nose Syndrome

White-Nose Syndrome

White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
Learn More
Mosquito, species not verified

Vector-Borne Diseases

Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center
Vector-Borne Diseases

Vector-Borne Diseases

Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
Learn More
Filter Total Items: 16
No Result Found
No results found.
Filter Total Items: 135

Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction

We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently...
Authors
P. Nol, J.L. Williamson, Tonie E. Rocke, Thomas M. Yuill
By
Ecosystems Mission Area, National Wildlife Health Center, Salton Sea

Recombinant raccoon pox vaccine protects mice against lethal plague Recombinant raccoon pox vaccine protects mice against lethal plague

Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the...
Authors
J.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, Tonie E. Rocke, D.T. Stinchcomb
By
Ecosystems Mission Area, National Wildlife Health Center

Determination of the median toxic dose of type C botulism in lactating dairy cows Determination of the median toxic dose of type C botulism in lactating dairy cows

Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose...
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, F.D. Galey, J.S. Cullor, A.A. Ardans
By
Ecosystems Mission Area, National Wildlife Health Center

New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos

An American white pelican (P. erythrorhyncos) was captured in August 1999 in California, USA. The bird was unable to fly away or evade the boat, and showed considerable aggression when captured. Numerous multifocal, 1 mm diameter, yellow masses were observed throughout the lungs and air sacs. The liver was yellow. A 6 cm diameter, firm spherical mass was present in the abdominal cavity...
Authors
P. Nol, R.E. Brannian, B.M. Berlowski, M. J. Wolcott, Tonie E. Rocke
By
Ecosystems Mission Area, National Wildlife Health Center

Failure to transmit avian vacuolar myelinopathy to mallard ducks Failure to transmit avian vacuolar myelinopathy to mallard ducks

Avian vacuolar myelinopathy (AVM) is a neurologic disease that has been diagnosed in free-ranging birds in the southeastern United States. Bald eagles (Haliaeetus leuocephalus), American coots (Fulica americana), and mallards (Anas platyrhynchos) have been affected. Previous investigations have not determined the etiology of this disease. In November and December 2002, we attempted to...
Authors
R. S. Larsen, Felicia B. Nutter, Tom Augspurger, Tonie E. Rocke, Nancy J. Thomas, Michael K. Stoskopf
By
Ecosystems Mission Area, National Wildlife Health Center

Vacuolar myelinopathy in waterfowl from a North Carolina impoundment Vacuolar myelinopathy in waterfowl from a North Carolina impoundment

Vacuolar myelinopathy was confirmed by light and electron microscopic examination of mallards (Anas platyrhynchos), ring-necked ducks (Aythya collaris), and buffleheads (Bucephala albeola) collected during an epizootic at Lake Surf in central North Carolina (USA) between November 1998 and February 1999. Clinical signs of affected birds were consistent with central nervous system...
Authors
T. Augspurger, John R. Fischer, Nancy Thomas, L. Sileo, Roger E. Brannian, Kimberli Miller, Tonie E. Rocke
By
Ecosystems Mission Area, National Wildlife Health Center

A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1 A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1

A serotype-specific polymerase chain reaction (PCR) assay was developed for detection and identification of Pasteurella multocida serotype 1, the causative agent of avian cholera in wild waterfowl. Arbitrarily primed PCR was used to detect DNA fragments that distinguish serotype 1 from the other 15 serotypes of P. multocida (with the exception of serotype 14). Oligonucleotide primers...
Authors
Tonie E. Rocke, S.R. Smith, A. Miyamoto, D.J. Shadduck
By
Ecosystems Mission Area, National Wildlife Health Center

Wildlife disease in the Colorado Delta as an indicator of ecosystem health Wildlife disease in the Colorado Delta as an indicator of ecosystem health

Ecosystem health and management are relatively new paradigms for environmental management (Costanza et al., 1992) and will be continually redefined and reevaluated relative to their purpose and utility in serving human society (Costanza et al., 1992; Sutter, 1993; Grumbine, 1994; Lackey, 1998, 1999). The stimulus for ecosystem management is often associated with sustaining or restoring...
Authors
Tonie E. Rocke, M. Friend
By
Ecosystems Mission Area, National Wildlife Health Center

PCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in PCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in

Botulism is one of the most important bird diseases world-wide and is caused by the intoxication with Botulinum-Neurotoxin-C1 (BoNt-C1), which is produced by toxigenic clostridia under appropriate conditions. Avian botulism leads regularly to large losses among the migrating bird populations breeding and resting at the saltwater pools of the Austrian national park Neusiedler See...
Authors
T.C. Zechmeister, A.H. Farnleitner, Tonie E. Rocke, F. Pittner, R. Rosengarten, R.L. Mach, A. Herzig, A.K.T. Kirschner
By
Ecosystems Mission Area, National Wildlife Health Center

Possible importance of algal toxins in the Salton Sea, California Possible importance of algal toxins in the Salton Sea, California

In response to wildlife mortality including unexplained eared grebe (Podiceps nigricollis) die-off events in 1992 and 1994 and other mortality events including large fish kills, a survey was conducted for the presence of algal toxins in the Salton Sea. Goals of this survey were to determine if and when algal toxins are present in the Salton Sea and to describe the phytoplankton...
Authors
Kristen M. Reifel, M. P. McCoy, Tonie E. Rocke, M. A. Tiffany, S. H. Hurlbert, D. J. Faulkner
By
Ecosystems Mission Area, National Wildlife Health Center, Salton Sea

Joining forces to improve our world Joining forces to improve our world

No abstract available.
Authors
W.B. Karesh, S.A. Osofsky, Tonie E. Rocke, P.L. Barrows
By
Ecosystems Mission Area, National Wildlife Health Center

A baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs A baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs

Laboratory and field studies were conducted between July and October 1999 to identify bait preference, biomarker efficacy, and bait acceptance rates for delivering an oral plague vaccine to black-tailed prairie dogs (Cynomys ludovicianus). Twenty juvenile captive prairie dogs were offered alfalfa baits containing either alfalfa, alfalfa and 5% molasses, or alfalfa, 5% molasses and 4%...
Authors
Terry E. Creekmore, Tonie E. Rocke, J. Hurley
By
Ecosystems Mission Area, National Wildlife Health Center
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