Tonie Rocke
Tonie Rocke is a Research Epidemiologist at the National Wildlife Health Center.
Science and Products
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Recombinant raccoon pox vaccine protects mice against lethal plague
Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of anti
Authors
J.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, T.E. Rocke, D.T. Stinchcomb
PCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in
Botulism is one of the most important bird diseases world-wide and is caused by the intoxication with Botulinum-Neurotoxin-C1 (BoNt-C1), which is produced by toxigenic clostridia under appropriate conditions. Avian botulism leads regularly to large losses among the migrating bird populations breeding and resting at the saltwater pools of the Austrian national park Neusiedler See-Seewinkel. Despite
Authors
T.C. Zechmeister, A.H. Farnleitner, T.E. Rocke, F. Pittner, R. Rosengarten, R.L. Mach, A. Herzig, A.K.T. Kirschner
Wildlife disease as an indicator for ecosystem health
Ecosystem health and management are relatively new paradigms for environmental management (Costanza et al., 1992) and will be continually redefined and reevaluated relative to their purpose and utility in serving human society (Costanza et al., 1992; Sutter, 1993; Grumbine, 1994; Lackey, 1998, 1999). The stimulus for ecosystem management is often associated with sustaining or restoring ecosystem h
Authors
T.E. Rocke, M. Friend
Possible importance of algal toxins in the Salton Sea, California
In response to wildlife mortality including unexplained eared grebe (Podiceps nigricollis) die-off events in 1992 and 1994 and other mortality events including large fish kills, a survey was conducted for the presence of algal toxins in the Salton Sea. Goals of this survey were to determine if and when algal toxins are present in the Salton Sea and to describe the phytoplankton composition during
Authors
Kristen M. Reifel, M. P. McCoy, Tonie E. Rocke, M. A. Tiffany, S. H. Hurlbert, D. J. Faulkner
Joining forces to improve our world
No abstract available.
Authors
W.B. Karesh, S.A. Osofsky, T.E. Rocke, P.L. Barrows
A baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs
Laboratory and field studies were conducted between July and October 1999 to identify bait preference, biomarker efficacy, and bait acceptance rates for delivering an oral plague vaccine to black-tailed prairie dogs (Cynomys ludovicianus). Twenty juvenile captive prairie dogs were offered alfalfa baits containing either alfalfa, alfalfa and 5% molasses, or alfalfa, 5% molasses and 4% salt. Based o
Authors
Terry E. Creekmore, Tonie E. Rocke, J. Hurley
Epizootiologic studies of avian vacuolar myelinopathy in waterbirds
Epizootic avian vacuolar myelinopathy (AVM) was first recognized as a neurologic disease in bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana) in Arkansas, USA in 1994 and 1996, respectively, but attempts to identify the etiology of the disease have been unsuccessful to date. Between 1998 and 2001, wing clipped sentinel birds (wild American coots and game farm mallards [A
Authors
Tonie E. Rocke, N. J. Thomas, T. Augspurger, Kimberli J.G. Miller
Clinical features of avian vacuolar myelinopathy in American coots
Objective—To characterize clinical features of avian vacuolar myelinopathy (AVM) in American coots.Design—Case-control study.Animals—26 AVM-affected American coots and 12 unaffected coots.Procedures—Complete physical, neurologic, hematologic, and plasma biochemical evaluations were performed. Affected coots received supportive care. All coots died or were euthanatized, and AVM status was confirmed
Authors
R. S. Larsen, Felicia B. Nutter, T. Augspurger, Tonie E. Rocke, L. Tomlinson, Nancy J. Thomas, M. K. Stoskopf
Avian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds
Wildlife biologists and health specialists have been frustrated by a long list of negative findings in their AVM investigations, however studies continue to provide pieces of information to aid the determination of the cause and its source. Available data indicated that AVM may have been present since at least 1990, occurs in at least five states, has been documented during October through April a
Authors
John R. Fischer, L.A. Lewis, T. Augspurger, T.E. Rocke
A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1
A serotype-specific polymerase chain reaction (PCR) assay was developed for detection and identification of Pasteurella multocida serotype 1, the causative agent of avian cholera in wild waterfowl. Arbitrarily primed PCR was used to detect DNA fragments that distinguish serotype 1 from the other 15 serotypes of P. multocida (with the exception of serotype 14). Oligonucleotide primers were construc
Authors
T.E. Rocke, S.R. Smith, A. Miyamoto, D.J. Shadduck
Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February-August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955-1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from surface fil
Authors
K.M. Reifel, M. P. McCoy, M. A. Tiffany, T.E. Rocke, C.C. Trees, S.B. Barlow, D. J. Faulkner, S. H. Hurlbert
Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February–August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955–1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from surface fil
Authors
Kristen M. Reifel, M. P. McCoy, M. A. Tiffany, Tonie E. Rocke, Charles Trees, S. B. Barlow, D. J. Faulkner, S. H. Hurlbert
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Recombinant raccoon pox vaccine protects mice against lethal plague
Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of antiAuthorsJ.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, T.E. Rocke, D.T. StinchcombPCR und ELISA - Alternativen zum Maustest für die Analyse des Botulismus-Neurotoxin-C1 Giftbildungspotentiales in Umweltproben? [PCR and ELISA - in vitro alternatives to the mouse-bioassay for assessing the botulinum-neurotoxin-C1 production potential in
Botulism is one of the most important bird diseases world-wide and is caused by the intoxication with Botulinum-Neurotoxin-C1 (BoNt-C1), which is produced by toxigenic clostridia under appropriate conditions. Avian botulism leads regularly to large losses among the migrating bird populations breeding and resting at the saltwater pools of the Austrian national park Neusiedler See-Seewinkel. DespiteAuthorsT.C. Zechmeister, A.H. Farnleitner, T.E. Rocke, F. Pittner, R. Rosengarten, R.L. Mach, A. Herzig, A.K.T. KirschnerWildlife disease as an indicator for ecosystem health
Ecosystem health and management are relatively new paradigms for environmental management (Costanza et al., 1992) and will be continually redefined and reevaluated relative to their purpose and utility in serving human society (Costanza et al., 1992; Sutter, 1993; Grumbine, 1994; Lackey, 1998, 1999). The stimulus for ecosystem management is often associated with sustaining or restoring ecosystem hAuthorsT.E. Rocke, M. FriendPossible importance of algal toxins in the Salton Sea, California
In response to wildlife mortality including unexplained eared grebe (Podiceps nigricollis) die-off events in 1992 and 1994 and other mortality events including large fish kills, a survey was conducted for the presence of algal toxins in the Salton Sea. Goals of this survey were to determine if and when algal toxins are present in the Salton Sea and to describe the phytoplankton composition duringAuthorsKristen M. Reifel, M. P. McCoy, Tonie E. Rocke, M. A. Tiffany, S. H. Hurlbert, D. J. FaulknerJoining forces to improve our world
No abstract available.AuthorsW.B. Karesh, S.A. Osofsky, T.E. Rocke, P.L. BarrowsA baiting system for delivery of an oral plague vaccine to black-tailed prairie dogs
Laboratory and field studies were conducted between July and October 1999 to identify bait preference, biomarker efficacy, and bait acceptance rates for delivering an oral plague vaccine to black-tailed prairie dogs (Cynomys ludovicianus). Twenty juvenile captive prairie dogs were offered alfalfa baits containing either alfalfa, alfalfa and 5% molasses, or alfalfa, 5% molasses and 4% salt. Based oAuthorsTerry E. Creekmore, Tonie E. Rocke, J. HurleyEpizootiologic studies of avian vacuolar myelinopathy in waterbirds
Epizootic avian vacuolar myelinopathy (AVM) was first recognized as a neurologic disease in bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana) in Arkansas, USA in 1994 and 1996, respectively, but attempts to identify the etiology of the disease have been unsuccessful to date. Between 1998 and 2001, wing clipped sentinel birds (wild American coots and game farm mallards [AAuthorsTonie E. Rocke, N. J. Thomas, T. Augspurger, Kimberli J.G. MillerClinical features of avian vacuolar myelinopathy in American coots
Objective—To characterize clinical features of avian vacuolar myelinopathy (AVM) in American coots.Design—Case-control study.Animals—26 AVM-affected American coots and 12 unaffected coots.Procedures—Complete physical, neurologic, hematologic, and plasma biochemical evaluations were performed. Affected coots received supportive care. All coots died or were euthanatized, and AVM status was confirmedAuthorsR. S. Larsen, Felicia B. Nutter, T. Augspurger, Tonie E. Rocke, L. Tomlinson, Nancy J. Thomas, M. K. StoskopfAvian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds
Wildlife biologists and health specialists have been frustrated by a long list of negative findings in their AVM investigations, however studies continue to provide pieces of information to aid the determination of the cause and its source. Available data indicated that AVM may have been present since at least 1990, occurs in at least five states, has been documented during October through April aAuthorsJohn R. Fischer, L.A. Lewis, T. Augspurger, T.E. RockeA serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1
A serotype-specific polymerase chain reaction (PCR) assay was developed for detection and identification of Pasteurella multocida serotype 1, the causative agent of avian cholera in wild waterfowl. Arbitrarily primed PCR was used to detect DNA fragments that distinguish serotype 1 from the other 15 serotypes of P. multocida (with the exception of serotype 14). Oligonucleotide primers were construcAuthorsT.E. Rocke, S.R. Smith, A. Miyamoto, D.J. ShadduckPleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February-August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955-1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from surface filAuthorsK.M. Reifel, M. P. McCoy, M. A. Tiffany, T.E. Rocke, C.C. Trees, S.B. Barlow, D. J. Faulkner, S. H. HurlbertPleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February–August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955–1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from surface filAuthorsKristen M. Reifel, M. P. McCoy, M. A. Tiffany, Tonie E. Rocke, Charles Trees, S. B. Barlow, D. J. Faulkner, S. H. Hurlbert - News