Tonie Rocke
Tonie Rocke is a Research Epidemiologist at the National Wildlife Health Center.
Science and Products
Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination
Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
White-nose Syndrome Vaccine Updates
Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
Field trials for testing of white-nose syndrome vaccine candidates
White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
Development of SARS-CoV-2 vaccine to support black-footed ferret conservation
The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela lutreola and Neovison vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics
Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
Vaccines
The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
Sylvatic Plague
Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
White-Nose Syndrome
White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
Vector-Borne Diseases
Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
Filter Total Items: 16
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Filter Total Items: 135
Epizootiologic studies of avian vacuolar myelinopathy in waterbirds Epizootiologic studies of avian vacuolar myelinopathy in waterbirds
Epizootic avian vacuolar myelinopathy (AVM) was first recognized as a neurologic disease in bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana) in Arkansas, USA in 1994 and 1996, respectively, but attempts to identify the etiology of the disease have been unsuccessful to date. Between 1998 and 2001, wing clipped sentinel birds (wild American coots and game farm...
Authors
Tonie E. Rocke, N. J. Thomas, T. Augspurger, Kimberli Miller
Clinical features of avian vacuolar myelinopathy in American coots Clinical features of avian vacuolar myelinopathy in American coots
Objective—To characterize clinical features of avian vacuolar myelinopathy (AVM) in American coots. Design—Case-control study. Animals—26 AVM-affected American coots and 12 unaffected coots. Procedures—Complete physical, neurologic, hematologic, and plasma biochemical evaluations were performed. Affected coots received supportive care. All coots died or were euthanatized, and AVM status...
Authors
R. S. Larsen, Felicia B. Nutter, T. Augspurger, Tonie E. Rocke, L. Tomlinson, Nancy J. Thomas, M. K. Stoskopf
Avian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds Avian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds
Wildlife biologists and health specialists have been frustrated by a long list of negative findings in their AVM investigations, however studies continue to provide pieces of information to aid the determination of the cause and its source. Available data indicated that AVM may have been present since at least 1990, occurs in at least five states, has been documented during October...
Authors
John R. Fischer, L.A. Lewis, T. Augspurger, Tonie E. Rocke
Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February–August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955–1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from...
Authors
Kristen M. Reifel, M. P. McCoy, M. A. Tiffany, Tonie E. Rocke, Charles Trees, S. B. Barlow, D. J. Faulkner, S. H. Hurlbert
Desert bighorn sheep mortality due to presumptive type C botulism in California Desert bighorn sheep mortality due to presumptive type C botulism in California
During a routine telemetry flight of the Mojave Desert (California, USA) in August 1995, mortality signals were detected from two of 12 radio-collared female desert bighorn sheep (Ovis canadensis) in the vicinity of Old Dad Peak in San Bernardino County (California). A series of field investigations determined that at least 45 bighorn sheep had died near two artificial water catchments...
Authors
P. K. Swift, J. D. Wehausen, H. B. Ernest, R. S. Singer, A. M. Pauli, H. Kinde, Tonie E. Rocke, V. C. Bleich
Efficacy of a type C botulism vaccine in green-winged teal Efficacy of a type C botulism vaccine in green-winged teal
We tested the efficacy of a single dose of Botumink toxoid for protecting wild green-winged teal (Anas crecca) during botulism epizootics caused by Clostridium botulinum type C. We challenged control and immunized ducks with four different doses of type C botulinum toxin to determine the LD50 for this species and to evaluate vaccine protection. Fewer immunized ducks were affected with...
Authors
Tonie E. Rocke, M.D. Samuel, P. K. Swift, G.S. Yarris
Type C botulism in dairy cattle from feed contaminated with a dead cat Type C botulism in dairy cattle from feed contaminated with a dead cat
Four hundred twenty-seven of 441 adult Holstein dairy cattle from a 1,200-cow dairy died over a 1-week period during early spring 1998. Affected animals were from 4 late lactation pens, one of which included the bull string. Signs included weakness, recumbency, watery diarrhea, and death. Eighty animals from the 4 pens were dead approximately 8 hours after the first ill cows were noted...
Authors
F.D. Galey, R. Terra, R. Walker, J. Adaska, M.A. Etchebarne, B. Puschener, R.H. Whitlock, Tonie E. Rocke, D. Willoughby, E. Tor
Water and sediment characteristics associated with avian botulism outbreaks in wetlands Water and sediment characteristics associated with avian botulism outbreaks in wetlands
Avian botulism kills thousands of waterbirds annually throughout North America, but management efforts to reduce its effects have been hindered because environmental conditions that promote outbreaks are poorly understood. We measured sediment and water variables in 32 pairs of wetlands with and without a current outbreak of avian botulism. Wetlands with botulism outbreaks had greater...
Authors
Tonie E. Rocke, Michael D. Samuel
Avian botulism Avian botulism
Avian botulism is a paralytic, often fatal, disease of birds that results when they ingest toxin produced by the bacterium, Clostridium botulinum. Seven distinct types of toxin designated by the letters A to G have been identified (Table 38.1). Waterfowl die-offs due to botulism are usually caused by type C toxin; sporadic die-offs among fish-eating birds, such as common loons and gulls...
Authors
Tonie E. Rocke, M. Friend
Oil Oil
Each year, an average of 14 million gallons of oil from more than 10,000 accidental spills flow into fresh and saltwater environments in and around the United States. Most accidental oil spills occur when oil is transported by tankers or barges, but oil is also spilled during highway, rail, and pipeline transport, and by nontransportation-related facilities, such as refinery, bulk...
Authors
Tonie E. Rocke
In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.
Authors
Judy L. Williamson, Tonie E. Rocke, Judd M. Aiken
Environmental characteristics associated with the occurrence of avian botulism in wetlands of a northern California refuge Environmental characteristics associated with the occurrence of avian botulism in wetlands of a northern California refuge
Avian botulism is an important disease affecting many species of waterbirds in North America, but the environmental conditions that initiate outbreaks are poorly understood. To determine wetland attributes associated with outbreaks of avian botulism in waterbirds at the Sacramento National Wildlife Refuge (SNWR), California, we compared environmental characteristics between wetlands...
Authors
Tonie E. Rocke, Ned H. Euliss, Michael D. Samuel
Science and Products
Dynamics of Rabies Transmission in Vampire Bats and Potential for Control Through Vaccination
Vampire bats are the primary spreader of rabies, a lethal disease that harms livestock and people across Latin America. Growth of the livestock industry and environmental changes in this region are leading to the expansion of vampire bats’ habitat range, and it is considered likely this species will move into the southern United States.
White-nose Syndrome Vaccine Updates
Since its emergence in 2006, white-nose syndrome (WNS) continues to spread in North America and has substantially impacted hibernating bat populations, contributing to declines of over 90% in northern long-eared ( Myotis septentrionalis), little brown ( Myotis lucifugus), and tri-colored bat ( Perimyotis subflavus) populations. We are developing tools and management strategies to protect bat...
Field trials for testing of white-nose syndrome vaccine candidates
White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), continues to decimate bat populations in North America, and efforts to develop treatment options have intensified. One potential method for controlling WNS is vaccination of bats with specific antigens to elicit a protective immune response.
Development of SARS-CoV-2 vaccine to support black-footed ferret conservation
The SARS-CoV-2 virus is known to infect and cause severe disease, such as respiratory distress and death, in mustelids, including farm-raised mink ( Mustela lutreola and Neovison vison) and domestic ferrets ( M. putorius). This raised concern that the virus may also pose a threat to endangered black-footed ferrets ( Mustela nigripes) managed in captivity for breeding and recovery purposes.
Modeling the response of cave hibernating Myotis species to white-nose syndrome mitigation tactics
Bat Research Research collaboration: Robin Russell (NWHC), Tonie Rocke (NWHC), Wayne Thogmartin (UMESC), Evan Grant (EESC) White-nose syndrome is a fungal disease devastating cave-hibernating bat species ( Myotis spp.) in the eastern United States. Several mitigation tactics have been proposed to alleviate the effects of white-nose syndrome on bats including probiotics and vaccination. Questions...
Vaccines
The USGS National Wildlife Health Center (NWHC) works on developing various disease management tools, including the development of vaccines. Our current work focuses on vaccines for sylvatic plague, white-nose syndrome, and rabies as disease control strategies.
Sylvatic Plague
Sylvatic plague, caused by Yersinia pestis, is a bacterial disease transmitted by fleas that afflicts many mammalian species, including humans.
White-Nose Syndrome
White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
Vector-Borne Diseases
Vector-borne diseases are transmitted from one animal to another by vectors, including insects, such as mosquitoes or fleas, and arachnids, such as ticks. The USGS National Wildlife Health Center investigates wildlife diseases, including vector-borne diseases, such as West Nile virus and sylvatic plague.
Filter Total Items: 16
No Result Found
Filter Total Items: 135
Epizootiologic studies of avian vacuolar myelinopathy in waterbirds Epizootiologic studies of avian vacuolar myelinopathy in waterbirds
Epizootic avian vacuolar myelinopathy (AVM) was first recognized as a neurologic disease in bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana) in Arkansas, USA in 1994 and 1996, respectively, but attempts to identify the etiology of the disease have been unsuccessful to date. Between 1998 and 2001, wing clipped sentinel birds (wild American coots and game farm...
Authors
Tonie E. Rocke, N. J. Thomas, T. Augspurger, Kimberli Miller
Clinical features of avian vacuolar myelinopathy in American coots Clinical features of avian vacuolar myelinopathy in American coots
Objective—To characterize clinical features of avian vacuolar myelinopathy (AVM) in American coots. Design—Case-control study. Animals—26 AVM-affected American coots and 12 unaffected coots. Procedures—Complete physical, neurologic, hematologic, and plasma biochemical evaluations were performed. Affected coots received supportive care. All coots died or were euthanatized, and AVM status...
Authors
R. S. Larsen, Felicia B. Nutter, T. Augspurger, Tonie E. Rocke, L. Tomlinson, Nancy J. Thomas, M. K. Stoskopf
Avian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds Avian vacuolar myelinopathy: a newly recognized fatal neurologic disease of eagles, waterfowl, and other birds
Wildlife biologists and health specialists have been frustrated by a long list of negative findings in their AVM investigations, however studies continue to provide pieces of information to aid the determination of the cause and its source. Available data indicated that AVM may have been present since at least 1990, occurs in at least five states, has been documented during October...
Authors
John R. Fischer, L.A. Lewis, T. Augspurger, Tonie E. Rocke
Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California Pleurochrysis pseudoroscoffensis (Prymnesiophyceae) blooms on the surface of the Salton Sea, California
Dense populations of the coccolithophore Pleurochrysis pseudoroscoffensis were found in surface films at several locations around the Salton Sea in February–August, 1999. An unidentified coccolithophorid was also found in low densities in earlier studies of the lake (1955–1956). To our knowledge, this is the first record of this widespread marine species in any lake. Samples taken from...
Authors
Kristen M. Reifel, M. P. McCoy, M. A. Tiffany, Tonie E. Rocke, Charles Trees, S. B. Barlow, D. J. Faulkner, S. H. Hurlbert
Desert bighorn sheep mortality due to presumptive type C botulism in California Desert bighorn sheep mortality due to presumptive type C botulism in California
During a routine telemetry flight of the Mojave Desert (California, USA) in August 1995, mortality signals were detected from two of 12 radio-collared female desert bighorn sheep (Ovis canadensis) in the vicinity of Old Dad Peak in San Bernardino County (California). A series of field investigations determined that at least 45 bighorn sheep had died near two artificial water catchments...
Authors
P. K. Swift, J. D. Wehausen, H. B. Ernest, R. S. Singer, A. M. Pauli, H. Kinde, Tonie E. Rocke, V. C. Bleich
Efficacy of a type C botulism vaccine in green-winged teal Efficacy of a type C botulism vaccine in green-winged teal
We tested the efficacy of a single dose of Botumink toxoid for protecting wild green-winged teal (Anas crecca) during botulism epizootics caused by Clostridium botulinum type C. We challenged control and immunized ducks with four different doses of type C botulinum toxin to determine the LD50 for this species and to evaluate vaccine protection. Fewer immunized ducks were affected with...
Authors
Tonie E. Rocke, M.D. Samuel, P. K. Swift, G.S. Yarris
Type C botulism in dairy cattle from feed contaminated with a dead cat Type C botulism in dairy cattle from feed contaminated with a dead cat
Four hundred twenty-seven of 441 adult Holstein dairy cattle from a 1,200-cow dairy died over a 1-week period during early spring 1998. Affected animals were from 4 late lactation pens, one of which included the bull string. Signs included weakness, recumbency, watery diarrhea, and death. Eighty animals from the 4 pens were dead approximately 8 hours after the first ill cows were noted...
Authors
F.D. Galey, R. Terra, R. Walker, J. Adaska, M.A. Etchebarne, B. Puschener, R.H. Whitlock, Tonie E. Rocke, D. Willoughby, E. Tor
Water and sediment characteristics associated with avian botulism outbreaks in wetlands Water and sediment characteristics associated with avian botulism outbreaks in wetlands
Avian botulism kills thousands of waterbirds annually throughout North America, but management efforts to reduce its effects have been hindered because environmental conditions that promote outbreaks are poorly understood. We measured sediment and water variables in 32 pairs of wetlands with and without a current outbreak of avian botulism. Wetlands with botulism outbreaks had greater...
Authors
Tonie E. Rocke, Michael D. Samuel
Avian botulism Avian botulism
Avian botulism is a paralytic, often fatal, disease of birds that results when they ingest toxin produced by the bacterium, Clostridium botulinum. Seven distinct types of toxin designated by the letters A to G have been identified (Table 38.1). Waterfowl die-offs due to botulism are usually caused by type C toxin; sporadic die-offs among fish-eating birds, such as common loons and gulls...
Authors
Tonie E. Rocke, M. Friend
Oil Oil
Each year, an average of 14 million gallons of oil from more than 10,000 accidental spills flow into fresh and saltwater environments in and around the United States. Most accidental oil spills occur when oil is transported by tankers or barges, but oil is also spilled during highway, rail, and pipeline transport, and by nontransportation-related facilities, such as refinery, bulk...
Authors
Tonie E. Rocke
In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.
Authors
Judy L. Williamson, Tonie E. Rocke, Judd M. Aiken
Environmental characteristics associated with the occurrence of avian botulism in wetlands of a northern California refuge Environmental characteristics associated with the occurrence of avian botulism in wetlands of a northern California refuge
Avian botulism is an important disease affecting many species of waterbirds in North America, but the environmental conditions that initiate outbreaks are poorly understood. To determine wetland attributes associated with outbreaks of avian botulism in waterbirds at the Sacramento National Wildlife Refuge (SNWR), California, we compared environmental characteristics between wetlands...
Authors
Tonie E. Rocke, Ned H. Euliss, Michael D. Samuel