The Molecular Ecology Laboratory applies genetic and genomic technologies to address a variety of complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. Together with our partners, we design and implement studies to document genetic diversity and the distribution of genetic variation among individuals, populations, and species. Information from these studies is used to support wildlife-management planning and conservation actions. Current and past studies have provided information to assess taxonomic boundaries, inform listing decisions made under the Endangered Species Act, identify unique or genetically depauperate populations, estimate population size or survival rates, develop management or recovery plans, breed wildlife in captivity, relocate wildlife from one location to another, and assess the effects of environmental change.
Conservation genomics is a new field of science that applies novel whole-genome sequencing technology to problems in conservation biology. Rapidly advancing molecular technologies are revolutionizing wildlife ecology, greatly expanding our understanding of wildlife and their interactions with the environment. In the same way that molecular tools such as microsatellites revolutionized wildlife management in the past, evolving genomic-level data collection techniques are beginning to offer powerful ways to assess biodiversity, taxonomy, hybridization, diets, demography, disease resistance and outbreaks, and even local adaptation.
Landscape genetics is a recently developed discipline that involves the merger of molecular population genetics and landscape ecology. The goal of this new field of study is to provide information about the interaction between landscape features and microevolutionary processes such as gene flow, genetic drift, and selection allowing for the understanding of processes that generate genetic structure across space.
Population genetics is an area of research that examines the distribution of genetic variation and levels of genetic diversity within and between populations. This information provides insights into the level of connectedness of populations throughout a species’ range and can be used to identify unique populations or those with low levels of genetic diversity.
Molecular tagging is a new application of molecular genetic techniques to traditional mark-recapture methodology designed to address situations where traditional methods fail. In such studies, non-invasively collected samples (such as feces, feathers, or fur) are used as a source of DNA that is then genotyped at multiple loci such that each individual animal can be uniquely identified. Thus, each individual’s DNA represents a unique tag analogous to a band or other mark used in traditional mark-recapture studies.
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and cryptic species.
Taxonomic uncertainty can be assessed using genetic data, along with other lines of evidence (such as morphological and behavioral characteristics). Such data can be used to identify and assess taxonomic boundaries (species, subspecies, hybrids) and in many cases redefine them. Such delineations are highly relevant for species status determinations (endangered, threatened, or at-risk).
Family Relationships and Mating Systems
Family relationships and mating systems can be investigated and defined using genetic data. This information is potentially important for conservation and management as it may influence effective population size and levels of genetic diversity.
Population models can incorporate genetic data to assess potential impacts of different management strategies on connectivity, effective population size, and genetic diversity.
Below are other science projects associated with this project.
Real-World Applications of Molecular Genetics
Conservation Genomics
Landscape Genetics
Population Genetics
Molecular Tagging
Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Taxonomic Uncertainty
Family Relationships and Mating Systems
Population Models
Below are multimedia items associated with this project.
Below are publications associated with this project.
Critical considerations for the application of environmental DNA methods to detect aquatic species
A field ornithologist’s guide to genomics: Practical considerations for ecology and conservation
Rangewide genetic analysis of Lesser Prairie-Chicken reveals population structure, range expansion, and possible introgression
Contrasting evolutionary histories of MHC class I and class II loci in grouse—Effects of selection and gene conversion
Z chromosome divergence, polymorphism and relative effective population size in a genus of lekking birds
Potential demographic and genetic effects of a sterilant applied to wild horse mares
Landscape characteristics influencing the genetic structure of greater sage-grouse within the stronghold of their range: a holistic modeling approach
Environmental DNA (eDNA) sampling improves occurrence and detection estimates of invasive Burmese pythons
Development of 13 microsatellites for Gunnison Sage-grouse (Centrocercus minimus) using next-generation shotgun sequencing and their utility in Greater Sage-grouse (Centrocercus urophasianus)
Genomic single-nucleotide polymorphisms confirm that Gunnison and Greater sage-grouse are genetically well differentiated and that the Bi-State population is distinct
Blood from a turnip: tissue origin of low-coverage shotgun sequencing libraries affects recovery of mitogenome sequences
Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies
Below are partners associated with this project.
The Molecular Ecology Laboratory applies genetic and genomic technologies to address a variety of complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. Together with our partners, we design and implement studies to document genetic diversity and the distribution of genetic variation among individuals, populations, and species. Information from these studies is used to support wildlife-management planning and conservation actions. Current and past studies have provided information to assess taxonomic boundaries, inform listing decisions made under the Endangered Species Act, identify unique or genetically depauperate populations, estimate population size or survival rates, develop management or recovery plans, breed wildlife in captivity, relocate wildlife from one location to another, and assess the effects of environmental change.
Conservation genomics is a new field of science that applies novel whole-genome sequencing technology to problems in conservation biology. Rapidly advancing molecular technologies are revolutionizing wildlife ecology, greatly expanding our understanding of wildlife and their interactions with the environment. In the same way that molecular tools such as microsatellites revolutionized wildlife management in the past, evolving genomic-level data collection techniques are beginning to offer powerful ways to assess biodiversity, taxonomy, hybridization, diets, demography, disease resistance and outbreaks, and even local adaptation.
Landscape genetics is a recently developed discipline that involves the merger of molecular population genetics and landscape ecology. The goal of this new field of study is to provide information about the interaction between landscape features and microevolutionary processes such as gene flow, genetic drift, and selection allowing for the understanding of processes that generate genetic structure across space.
Population genetics is an area of research that examines the distribution of genetic variation and levels of genetic diversity within and between populations. This information provides insights into the level of connectedness of populations throughout a species’ range and can be used to identify unique populations or those with low levels of genetic diversity.
Molecular tagging is a new application of molecular genetic techniques to traditional mark-recapture methodology designed to address situations where traditional methods fail. In such studies, non-invasively collected samples (such as feces, feathers, or fur) are used as a source of DNA that is then genotyped at multiple loci such that each individual animal can be uniquely identified. Thus, each individual’s DNA represents a unique tag analogous to a band or other mark used in traditional mark-recapture studies.
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and cryptic species.
Taxonomic uncertainty can be assessed using genetic data, along with other lines of evidence (such as morphological and behavioral characteristics). Such data can be used to identify and assess taxonomic boundaries (species, subspecies, hybrids) and in many cases redefine them. Such delineations are highly relevant for species status determinations (endangered, threatened, or at-risk).
Family Relationships and Mating Systems
Family relationships and mating systems can be investigated and defined using genetic data. This information is potentially important for conservation and management as it may influence effective population size and levels of genetic diversity.
Population models can incorporate genetic data to assess potential impacts of different management strategies on connectivity, effective population size, and genetic diversity.
Below are other science projects associated with this project.
Real-World Applications of Molecular Genetics
Conservation Genomics
Landscape Genetics
Population Genetics
Molecular Tagging
Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Taxonomic Uncertainty
Family Relationships and Mating Systems
Population Models
Below are multimedia items associated with this project.
Below are publications associated with this project.
Critical considerations for the application of environmental DNA methods to detect aquatic species
A field ornithologist’s guide to genomics: Practical considerations for ecology and conservation
Rangewide genetic analysis of Lesser Prairie-Chicken reveals population structure, range expansion, and possible introgression
Contrasting evolutionary histories of MHC class I and class II loci in grouse—Effects of selection and gene conversion
Z chromosome divergence, polymorphism and relative effective population size in a genus of lekking birds
Potential demographic and genetic effects of a sterilant applied to wild horse mares
Landscape characteristics influencing the genetic structure of greater sage-grouse within the stronghold of their range: a holistic modeling approach
Environmental DNA (eDNA) sampling improves occurrence and detection estimates of invasive Burmese pythons
Development of 13 microsatellites for Gunnison Sage-grouse (Centrocercus minimus) using next-generation shotgun sequencing and their utility in Greater Sage-grouse (Centrocercus urophasianus)
Genomic single-nucleotide polymorphisms confirm that Gunnison and Greater sage-grouse are genetically well differentiated and that the Bi-State population is distinct
Blood from a turnip: tissue origin of low-coverage shotgun sequencing libraries affects recovery of mitogenome sequences
Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies
Below are partners associated with this project.