The Molecular Ecology Laboratory applies genetic and genomic technologies to address a variety of complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. Together with our partners, we design and implement studies to document genetic diversity and the distribution of genetic variation among individuals, populations, and species. Information from these studies is used to support wildlife-management planning and conservation actions. Current and past studies have provided information to assess taxonomic boundaries, inform listing decisions made under the Endangered Species Act, identify unique or genetically depauperate populations, estimate population size or survival rates, develop management or recovery plans, breed wildlife in captivity, relocate wildlife from one location to another, and assess the effects of environmental change.
Conservation genomics is a new field of science that applies novel whole-genome sequencing technology to problems in conservation biology. Rapidly advancing molecular technologies are revolutionizing wildlife ecology, greatly expanding our understanding of wildlife and their interactions with the environment. In the same way that molecular tools such as microsatellites revolutionized wildlife management in the past, evolving genomic-level data collection techniques are beginning to offer powerful ways to assess biodiversity, taxonomy, hybridization, diets, demography, disease resistance and outbreaks, and even local adaptation.
Landscape genetics is a recently developed discipline that involves the merger of molecular population genetics and landscape ecology. The goal of this new field of study is to provide information about the interaction between landscape features and microevolutionary processes such as gene flow, genetic drift, and selection allowing for the understanding of processes that generate genetic structure across space.
Population genetics is an area of research that examines the distribution of genetic variation and levels of genetic diversity within and between populations. This information provides insights into the level of connectedness of populations throughout a species’ range and can be used to identify unique populations or those with low levels of genetic diversity.
Molecular tagging is a new application of molecular genetic techniques to traditional mark-recapture methodology designed to address situations where traditional methods fail. In such studies, non-invasively collected samples (such as feces, feathers, or fur) are used as a source of DNA that is then genotyped at multiple loci such that each individual animal can be uniquely identified. Thus, each individual’s DNA represents a unique tag analogous to a band or other mark used in traditional mark-recapture studies.
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and cryptic species.
Taxonomic uncertainty can be assessed using genetic data, along with other lines of evidence (such as morphological and behavioral characteristics). Such data can be used to identify and assess taxonomic boundaries (species, subspecies, hybrids) and in many cases redefine them. Such delineations are highly relevant for species status determinations (endangered, threatened, or at-risk).
Family Relationships and Mating Systems
Family relationships and mating systems can be investigated and defined using genetic data. This information is potentially important for conservation and management as it may influence effective population size and levels of genetic diversity.
Population models can incorporate genetic data to assess potential impacts of different management strategies on connectivity, effective population size, and genetic diversity.
Below are other science projects associated with this project.
Population Genetics
Molecular Tagging
Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Taxonomic Uncertainty
Family Relationships and Mating Systems
Population Models
Landscape Influence on Gene Flow in Greater Sage-grouse
Greater sage-grouse network-prioritized functional connectivity cumulative current map (raster)
Metabarcode sequencing of aquatic environmental DNA from the Potomac River Watershed, 2015-2020
Microsatellite data, boundaries of subpopulation centers, and estimated effective migration for greater sage-grouse collected in western North America between 1992 and 2015 (ver. 2.0, December 2022)
A reference genome assembly for the endangered Aga or Mariana Crow (Corvus kubaryi)
Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms
qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming
Gene annotations for the hoary bat (Lasiurus [Aeorestes] cinereus) and alignments with other bat gene sets for evolutionary analysis
Hepatic Transcriptome of Japanese quail (Coturnix japonica) Exposed to 17B.-Trenbolone
Genetic variation in hoary bats (Lasiurus cinereus) assessed from archived samples
Sample collection information and SNP data for Gunnison Sage-grouse across the species range generated in the Molecular Ecology Lab during 2015-2018
Sample collection information and microsatellite data for Gunnison sage-grouse pre and post translocation
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Below are multimedia items associated with this project.
Fecal metabarcoding of the endangered Pacific pocket mouse (Perognathus longimembris pacificus) reveals a diverse and forb rich diet that reflects local habitat availability
Genetic mark–recapture analysis reveals large annual variation in pre-breeding sex ratio of greater sage-grouse
Assessing arthropod diversity metrics derived from stream environmental DNA: Spatiotemporal variation and paired comparisons with manual sampling
Simulation of genetic change under four removal strategies for a wild horse population
The ties that bind the sagebrush biome: Integrating genetic connectivity into range-wide conservation of greater sage-grouse
Comparison of microscopy and metabarcoding to identify pollen used by the critically endangered rusty patched bumble bee, Bombus affinis
A genetic warning system for a hierarchically structured wildlife monitoring framework
Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas
Positively selected genes in the hoary bat (Lasiurus cinereus) lineage: Prominence of thymus expression, immune and metabolic function, and regions of ancient synteny
Influence of permafrost type and site history on losses of permafrost carbon after thaw
Honey bee foraged pollen reveals temporal changes in pollen protein content and changes in forager choice for abundant versus high protein flowers
Sex- and developmental stage-related differences in the hepatic transcriptome of Japanese quail (Coturnix japonica) exposed to 17β-Trenbolone
Below are partners associated with this project.
The Molecular Ecology Laboratory applies genetic and genomic technologies to address a variety of complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. Together with our partners, we design and implement studies to document genetic diversity and the distribution of genetic variation among individuals, populations, and species. Information from these studies is used to support wildlife-management planning and conservation actions. Current and past studies have provided information to assess taxonomic boundaries, inform listing decisions made under the Endangered Species Act, identify unique or genetically depauperate populations, estimate population size or survival rates, develop management or recovery plans, breed wildlife in captivity, relocate wildlife from one location to another, and assess the effects of environmental change.
Conservation genomics is a new field of science that applies novel whole-genome sequencing technology to problems in conservation biology. Rapidly advancing molecular technologies are revolutionizing wildlife ecology, greatly expanding our understanding of wildlife and their interactions with the environment. In the same way that molecular tools such as microsatellites revolutionized wildlife management in the past, evolving genomic-level data collection techniques are beginning to offer powerful ways to assess biodiversity, taxonomy, hybridization, diets, demography, disease resistance and outbreaks, and even local adaptation.
Landscape genetics is a recently developed discipline that involves the merger of molecular population genetics and landscape ecology. The goal of this new field of study is to provide information about the interaction between landscape features and microevolutionary processes such as gene flow, genetic drift, and selection allowing for the understanding of processes that generate genetic structure across space.
Population genetics is an area of research that examines the distribution of genetic variation and levels of genetic diversity within and between populations. This information provides insights into the level of connectedness of populations throughout a species’ range and can be used to identify unique populations or those with low levels of genetic diversity.
Molecular tagging is a new application of molecular genetic techniques to traditional mark-recapture methodology designed to address situations where traditional methods fail. In such studies, non-invasively collected samples (such as feces, feathers, or fur) are used as a source of DNA that is then genotyped at multiple loci such that each individual animal can be uniquely identified. Thus, each individual’s DNA represents a unique tag analogous to a band or other mark used in traditional mark-recapture studies.
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and cryptic species.
Taxonomic uncertainty can be assessed using genetic data, along with other lines of evidence (such as morphological and behavioral characteristics). Such data can be used to identify and assess taxonomic boundaries (species, subspecies, hybrids) and in many cases redefine them. Such delineations are highly relevant for species status determinations (endangered, threatened, or at-risk).
Family Relationships and Mating Systems
Family relationships and mating systems can be investigated and defined using genetic data. This information is potentially important for conservation and management as it may influence effective population size and levels of genetic diversity.
Population models can incorporate genetic data to assess potential impacts of different management strategies on connectivity, effective population size, and genetic diversity.
Below are other science projects associated with this project.
Population Genetics
Molecular Tagging
Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Taxonomic Uncertainty
Family Relationships and Mating Systems
Population Models
Landscape Influence on Gene Flow in Greater Sage-grouse
Greater sage-grouse network-prioritized functional connectivity cumulative current map (raster)
Metabarcode sequencing of aquatic environmental DNA from the Potomac River Watershed, 2015-2020
Microsatellite data, boundaries of subpopulation centers, and estimated effective migration for greater sage-grouse collected in western North America between 1992 and 2015 (ver. 2.0, December 2022)
A reference genome assembly for the endangered Aga or Mariana Crow (Corvus kubaryi)
Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms
qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming
Gene annotations for the hoary bat (Lasiurus [Aeorestes] cinereus) and alignments with other bat gene sets for evolutionary analysis
Hepatic Transcriptome of Japanese quail (Coturnix japonica) Exposed to 17B.-Trenbolone
Genetic variation in hoary bats (Lasiurus cinereus) assessed from archived samples
Sample collection information and SNP data for Gunnison Sage-grouse across the species range generated in the Molecular Ecology Lab during 2015-2018
Sample collection information and microsatellite data for Gunnison sage-grouse pre and post translocation
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Below are multimedia items associated with this project.
Fecal metabarcoding of the endangered Pacific pocket mouse (Perognathus longimembris pacificus) reveals a diverse and forb rich diet that reflects local habitat availability
Genetic mark–recapture analysis reveals large annual variation in pre-breeding sex ratio of greater sage-grouse
Assessing arthropod diversity metrics derived from stream environmental DNA: Spatiotemporal variation and paired comparisons with manual sampling
Simulation of genetic change under four removal strategies for a wild horse population
The ties that bind the sagebrush biome: Integrating genetic connectivity into range-wide conservation of greater sage-grouse
Comparison of microscopy and metabarcoding to identify pollen used by the critically endangered rusty patched bumble bee, Bombus affinis
A genetic warning system for a hierarchically structured wildlife monitoring framework
Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas
Positively selected genes in the hoary bat (Lasiurus cinereus) lineage: Prominence of thymus expression, immune and metabolic function, and regions of ancient synteny
Influence of permafrost type and site history on losses of permafrost carbon after thaw
Honey bee foraged pollen reveals temporal changes in pollen protein content and changes in forager choice for abundant versus high protein flowers
Sex- and developmental stage-related differences in the hepatic transcriptome of Japanese quail (Coturnix japonica) exposed to 17β-Trenbolone
Below are partners associated with this project.