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The USGS National Wildlife Health Center (NWHC) assists State, Federal, and Tribal wildlife agencies nationwide with early detection of Pseudogymnoascus destructans (Pd), and addresses specific research priorities identified by partners in conjunction with the White-Nose Syndrome National Plan.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA. (Credit: Katrien Werner, USGS National Wildlife Health Center. Public domain.)
During annual bat population surveys, participating agencies collect swabs of bat skin, guano, hibernaculum sediment, and environmental substrate. If clinical signs of white-nose syndrome (WNS) are observed in the population, carcasses or wing biopsies from affected bats are collected for diagnostic testing.
Preliminary characterization of risk factors associated with Pd movement suggest that new detections are related to the distance to nearest known Pd-positive sites and only mildly associated with the size of a site’s hibernating bat population. We are also investigating use of community guano samples from summer roosts for Pd surveillance. This strategy has particularly utility for areas where bat hibernation sites are not known or are difficult to access.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance. (Credit: Kyle George, USGS National Wildlife Health Center. Public domain.)
In 2012 NWHC scientists collected swabs from bat wings, cave walls, and equipment used in and near study sites in Kentucky, Indiana, Ohio, Tennessee, and Virginia. They also collected guano from individual bats and sediment from the floor of underground summer roost sites. NWHC scientists found that bats occupying hibernation sites in summer can harbor Pd on their skin, and that Pd is more readily detectable in their guano. More findings are summarized in a USGS News Release: Deadly fungus affecting hibernating bats could spread during summer.
Despite active national surveillance efforts to detect the spread of WNS, the 2016 detection of WNS in Washington State illustrates the ongoing importance of investigating wildlife mortality events as part of a comprehensive wildlife disease surveillance strategy, and wildlife managers are encouraged to report unusual bat mortality or bats displaying clinical signs suggestive of WNS to the NWHC for further investigation. NWHC can also answer questions about designing WNS surveillance and response plans relevant to your state and help with testing samples collected as part of opportunistic or targeted surveillance efforts in accordance with the national Pd surveillance strategy.
If you are a private individual or entity with a question about white-nose syndrome in your area, please contact your state department of natural resources or state agency of game and fish. A list of State and Federal agencies can be found at fishwildlife.org.
If you are from a State, Federal, or Tribal agency, please see more information on reporting mortality events and submitting samples related to white-nose syndrome. Tribal, State, and Federal agencies with questions about ongoing surveillance efforts, or who may wish to participate, should contact nwhc-epi@usgs.gov.
More resources on white-nose syndrome surveillance:
The USGS National Wildlife Health Center, using samples collected by state, federal, and tribal partners as part of the white-nose syndrome/Pd fungus surveillance, assessed the genetic diversity of bat coronaviruses in North America.
Investigating coronaviruses and white-nose syndrome fungus in free-ranging North American bats
The USGS National Wildlife Health Center, using samples collected by state, federal, and tribal partners as part of the white-nose syndrome/Pd fungus surveillance, assessed the genetic diversity of bat coronaviruses in North America.
The USGS National Wildlife Health Center, in partnership with the White-nose Syndrome (WNS) National Response Team Diagnostic Working Group, has developed the White-Nose Syndrome Diagnostic Laboratory Network Handbook to harmonize WNS diagnostics.
The USGS National Wildlife Health Center, in partnership with the White-nose Syndrome (WNS) National Response Team Diagnostic Working Group, has developed the White-Nose Syndrome Diagnostic Laboratory Network Handbook to harmonize WNS diagnostics.
Collecting a skin swab for white-nose syndrome surveillance
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
Approved euthanasia methods for bats (Microchiroptera)
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
Tables are placed below bat boxes to collect guano from roosting bats
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Scientist measuring the length of the forearm of a bat
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
Fungal diseases are an emerging global problem affecting human health, food security and biodiversity. Ability of many fungal pathogens to persist within environmental reservoirs can increase extinction risks for host species and presents challenges for disease control. Understanding factors that regulate pathogen spread and persistence in these reservoirs is critical for effective...
Authors
Michelle L. Verant, Elizabeth A. Bohuski, Katherine L. D. Richgels, Kevin J. Olival, Jonathan H. Epstein, David S. Blehert
Bats occupying hibernacula during summer are exposed to Pseudogymnoascus destructans (Pd), the causative agent of white-nose syndrome (WNS), and may contribute to its dispersal. Furthermore, equipment and clothing exposed to cave environments are a potential source for human-assisted spread of Pd. To explore dispersal hazards for Pd during the nonhibernal season, we tested samples that...
Authors
Anne Ballmann, Miranda R. Torkelson, Elizabeth A. Bohuski, Robin E. Russell, David S. Blehert
White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia...
Authors
Jeffrey M. Lorch, Jonathan M. Palmer, Daniel L. Lindner, Anne Ballmann, Kyle George, Kathryn M. Griffin, Susan Knowles, John R. Huckabee, Katherine H. Haman, Christopher D. Anderson, Penny A. Becker, Joseph B. Buchanan, Jeffrey T. Foster, David S. Blehert
The continued spread of white-nose syndrome and its impacts on hibernating bat populations across North America has prompted nationwide surveillance efforts and the need for high-throughput, noninvasive diagnostic tools. Quantitative real-time polymerase chain reaction (qPCR) analysis has been increasingly used for detection of the causative fungus, Pseudogymnoascus destructans, in both...
Authors
Michelle Verant, Elizabeth A. Bohuski, Jeffrey M. Lorch, David S. Blehert
The recent emergence of white-nose syndrome (WNS), a fungal disease causing unprecedented mortality among hibernating bats of eastern North America, has revealed a knowledge gap regarding fungal communities associated with bats and their hibernacula. We used culture-based techniques to investigate the diversity of fungi in soil samples collected from 24 bat hibernacula in the eastern...
Authors
Jeffrey M. Lorch, Daniel L. Lindner, Andrea Gargas, Laura K. Muller, Andrew M. Minnis, David S. Blehert
The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as...
Authors
Laura K. Muller, Jeffrey M. Lorch, Daniel L. Lindner, Michael O’Connor, Andrea Gargas, David S. Blehert
White-nose syndrome (WNS) is an emerging disease causing unprecedented morbidity and mortality among bats in eastern North America. The disease is characterized by cutaneous infection of hibernating bats by the psychrophilic fungus Geomyces destructans. Detection of G. destructans in environments occupied by bats will be critical for WNS surveillance, management and characterization of...
Authors
Daniel L. Lindner, Andrea Gargas, Jeffrey M. Lorch, Mark T. Banik, Jessie Glaeser, Thomas H. Kunz, David S. Blehert
The USGS National Wildlife Health Center (NWHC) assists State, Federal, and Tribal wildlife agencies nationwide with early detection of Pseudogymnoascus destructans (Pd), and addresses specific research priorities identified by partners in conjunction with the White-Nose Syndrome National Plan.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA. (Credit: Katrien Werner, USGS National Wildlife Health Center. Public domain.)
During annual bat population surveys, participating agencies collect swabs of bat skin, guano, hibernaculum sediment, and environmental substrate. If clinical signs of white-nose syndrome (WNS) are observed in the population, carcasses or wing biopsies from affected bats are collected for diagnostic testing.
Preliminary characterization of risk factors associated with Pd movement suggest that new detections are related to the distance to nearest known Pd-positive sites and only mildly associated with the size of a site’s hibernating bat population. We are also investigating use of community guano samples from summer roosts for Pd surveillance. This strategy has particularly utility for areas where bat hibernation sites are not known or are difficult to access.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance. (Credit: Kyle George, USGS National Wildlife Health Center. Public domain.)
In 2012 NWHC scientists collected swabs from bat wings, cave walls, and equipment used in and near study sites in Kentucky, Indiana, Ohio, Tennessee, and Virginia. They also collected guano from individual bats and sediment from the floor of underground summer roost sites. NWHC scientists found that bats occupying hibernation sites in summer can harbor Pd on their skin, and that Pd is more readily detectable in their guano. More findings are summarized in a USGS News Release: Deadly fungus affecting hibernating bats could spread during summer.
Despite active national surveillance efforts to detect the spread of WNS, the 2016 detection of WNS in Washington State illustrates the ongoing importance of investigating wildlife mortality events as part of a comprehensive wildlife disease surveillance strategy, and wildlife managers are encouraged to report unusual bat mortality or bats displaying clinical signs suggestive of WNS to the NWHC for further investigation. NWHC can also answer questions about designing WNS surveillance and response plans relevant to your state and help with testing samples collected as part of opportunistic or targeted surveillance efforts in accordance with the national Pd surveillance strategy.
If you are a private individual or entity with a question about white-nose syndrome in your area, please contact your state department of natural resources or state agency of game and fish. A list of State and Federal agencies can be found at fishwildlife.org.
If you are from a State, Federal, or Tribal agency, please see more information on reporting mortality events and submitting samples related to white-nose syndrome. Tribal, State, and Federal agencies with questions about ongoing surveillance efforts, or who may wish to participate, should contact nwhc-epi@usgs.gov.
More resources on white-nose syndrome surveillance:
The USGS National Wildlife Health Center, using samples collected by state, federal, and tribal partners as part of the white-nose syndrome/Pd fungus surveillance, assessed the genetic diversity of bat coronaviruses in North America.
Investigating coronaviruses and white-nose syndrome fungus in free-ranging North American bats
The USGS National Wildlife Health Center, using samples collected by state, federal, and tribal partners as part of the white-nose syndrome/Pd fungus surveillance, assessed the genetic diversity of bat coronaviruses in North America.
The USGS National Wildlife Health Center, in partnership with the White-nose Syndrome (WNS) National Response Team Diagnostic Working Group, has developed the White-Nose Syndrome Diagnostic Laboratory Network Handbook to harmonize WNS diagnostics.
The USGS National Wildlife Health Center, in partnership with the White-nose Syndrome (WNS) National Response Team Diagnostic Working Group, has developed the White-Nose Syndrome Diagnostic Laboratory Network Handbook to harmonize WNS diagnostics.
Collecting a skin swab for white-nose syndrome surveillance
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
Approved euthanasia methods for bats (Microchiroptera)
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
Tables are placed below bat boxes to collect guano from roosting bats
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Scientist measuring the length of the forearm of a bat
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
Fungal diseases are an emerging global problem affecting human health, food security and biodiversity. Ability of many fungal pathogens to persist within environmental reservoirs can increase extinction risks for host species and presents challenges for disease control. Understanding factors that regulate pathogen spread and persistence in these reservoirs is critical for effective...
Authors
Michelle L. Verant, Elizabeth A. Bohuski, Katherine L. D. Richgels, Kevin J. Olival, Jonathan H. Epstein, David S. Blehert
Bats occupying hibernacula during summer are exposed to Pseudogymnoascus destructans (Pd), the causative agent of white-nose syndrome (WNS), and may contribute to its dispersal. Furthermore, equipment and clothing exposed to cave environments are a potential source for human-assisted spread of Pd. To explore dispersal hazards for Pd during the nonhibernal season, we tested samples that...
Authors
Anne Ballmann, Miranda R. Torkelson, Elizabeth A. Bohuski, Robin E. Russell, David S. Blehert
White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia...
Authors
Jeffrey M. Lorch, Jonathan M. Palmer, Daniel L. Lindner, Anne Ballmann, Kyle George, Kathryn M. Griffin, Susan Knowles, John R. Huckabee, Katherine H. Haman, Christopher D. Anderson, Penny A. Becker, Joseph B. Buchanan, Jeffrey T. Foster, David S. Blehert
The continued spread of white-nose syndrome and its impacts on hibernating bat populations across North America has prompted nationwide surveillance efforts and the need for high-throughput, noninvasive diagnostic tools. Quantitative real-time polymerase chain reaction (qPCR) analysis has been increasingly used for detection of the causative fungus, Pseudogymnoascus destructans, in both...
Authors
Michelle Verant, Elizabeth A. Bohuski, Jeffrey M. Lorch, David S. Blehert
The recent emergence of white-nose syndrome (WNS), a fungal disease causing unprecedented mortality among hibernating bats of eastern North America, has revealed a knowledge gap regarding fungal communities associated with bats and their hibernacula. We used culture-based techniques to investigate the diversity of fungi in soil samples collected from 24 bat hibernacula in the eastern...
Authors
Jeffrey M. Lorch, Daniel L. Lindner, Andrea Gargas, Laura K. Muller, Andrew M. Minnis, David S. Blehert
The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as...
Authors
Laura K. Muller, Jeffrey M. Lorch, Daniel L. Lindner, Michael O’Connor, Andrea Gargas, David S. Blehert
White-nose syndrome (WNS) is an emerging disease causing unprecedented morbidity and mortality among bats in eastern North America. The disease is characterized by cutaneous infection of hibernating bats by the psychrophilic fungus Geomyces destructans. Detection of G. destructans in environments occupied by bats will be critical for WNS surveillance, management and characterization of...
Authors
Daniel L. Lindner, Andrea Gargas, Jeffrey M. Lorch, Mark T. Banik, Jessie Glaeser, Thomas H. Kunz, David S. Blehert