Long-wave ultraviolet (UV) light can be used to detect orange fluorescence associated with white-nose syndrome (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome-surveillance) on bat skin.
The USGS National Wildlife Health Center (NWHC) assists State, Federal, and Tribal wildlife agencies nationwide with early detection of Pseudogymnoascus destructans (Pd), and addresses specific research priorities identified by partners in conjunction with the White-Nose Syndrome National Plan.
During annual bat population surveys, participating agencies collect swabs of bat skin, guano, hibernaculum sediment, and environmental substrate. If clinical signs of white-nose syndrome (WNS) are observed in the population, carcasses or wing biopsies from affected bats are collected for diagnostic testing.
Preliminary characterization of risk factors associated with Pd movement suggest that new detections are related to the distance to nearest known Pd-positive sites and only mildly associated with the size of a site’s hibernating bat population. We are also investigating use of community guano samples from summer roosts for Pd surveillance. This strategy has particularly utility for areas where bat hibernation sites are not known or are difficult to access.
In 2012 NWHC scientists collected swabs from bat wings, cave walls, and equipment used in and near study sites in Kentucky, Indiana, Ohio, Tennessee, and Virginia. They also collected guano from individual bats and sediment from the floor of underground summer roost sites. NWHC scientists found that bats occupying hibernation sites in summer can harbor Pd on their skin, and that Pd is more readily detectable in their guano. More findings are summarized in a USGS News Release: Deadly fungus affecting hibernating bats could spread during summer.
Despite active national surveillance efforts to detect the spread of WNS, the 2016 detection of WNS in Washington State illustrates the ongoing importance of investigating wildlife mortality events as part of a comprehensive wildlife disease surveillance strategy, and wildlife managers are encouraged to report unusual bat mortality or bats displaying clinical signs suggestive of WNS to the NWHC for further investigation. NWHC can also answer questions about designing WNS surveillance and response plans relevant to your state and help with testing samples collected as part of opportunistic or targeted surveillance efforts in accordance with the national Pd surveillance strategy.
If you are a private individual or entity with a question about white-nose syndrome in your area, please contact your state department of natural resources or state agency of game and fish. A list of State and Federal agencies can be found at fishwildlife.org.
If you are from a State, Federal, or Tribal agency, please see more information on reporting mortality events and submitting samples related to white-nose syndrome. Tribal, State, and Federal agencies with questions about ongoing surveillance efforts, or who may wish to participate, should contact nwhc-epi@usgs.gov.
More resources on white-nose syndrome surveillance:
- NWHC Bat White-Nose Syndrome/Pd Surveillance Submission Guidelines
- A National Plan for Assisting States, Federal Agencies, and Tribes in Managing White-Nose Syndrome in Bats
- White-Nose Syndrome.org - A Coordinated Response to the Devastating Bat Disease
- White-nose Syndrome Occurrence Map
- White-nose Syndrome Case Definitions
- Biosafety Guidance for Handling and Storage of Pseudogymnoascus destructans
- USFWS Surveillance and Diagnostics Working Group
White-nose syndrome surveillance training videos
Investigating coronaviruses and white-nose syndrome fungus in free-ranging North American bats
White-nose syndrome diagnostic harmonization
White-Nose Syndrome
Below are photos related to white-nose syndrome surveillance.
More photos related to white-nose syndrome are available in the WNS Image Gallery.

Long-wave ultraviolet (UV) light can be used to detect orange fluorescence associated with white-nose syndrome (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome-surveillance) on bat skin.

Environmental samples collected in or near bat roosting locations can be used to supplement samples collected from bats for surveillance for white-nose syndrome (WNS) (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome).
Environmental samples collected in or near bat roosting locations can be used to supplement samples collected from bats for surveillance for white-nose syndrome (WNS) (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome).

This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.

This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.

This video demonstrates the proper procedure for collecting a skin biopsy from a bat when conducting white-nose syndrome surveillance.
This video demonstrates the proper procedure for collecting a skin biopsy from a bat when conducting white-nose syndrome surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.

Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
A little brown bat is being released shortly after samples are collected.
A little brown bat is being released shortly after samples are collected.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
Mist-net and harp trap set up to capture bats for white-nose syndrome surveillance activities. Breckinridge County, KY. July 2012.
Mist-net and harp trap set up to capture bats for white-nose syndrome surveillance activities. Breckinridge County, KY. July 2012.
Scientist taking environmental samples during white-nose syndrome surveillance activities. Breckinridge County, IN. July 2012.
Scientist taking environmental samples during white-nose syndrome surveillance activities. Breckinridge County, IN. July 2012.
Examining the wing of a bat. Monroe County, IN. July 2012.
Examining the wing of a bat. Monroe County, IN. July 2012.
Below are publications about white-nose syndrome.
Determinants of Pseudogymnoascus destructans within bat hibernacula: Implications for surveillance and management of white-nose syndrome
Dispersal hazards of Pseudogymnoascus destructans by bats and human activity at hibernacula in summer
First detection of bat white-nose syndrome in western North America
Optimized methods for total nucleic acid extraction and quantification of the bat white-nose syndrome fungus, Pseudogymnoascus destructans, from swab and environmental samples
A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome
Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructanstructans.
DNA-based detection of the fungal pathogen Geomyces destructans in soil from bat hibernacula
Below are news stories about white-nose syndrome.
The USGS National Wildlife Health Center (NWHC) assists State, Federal, and Tribal wildlife agencies nationwide with early detection of Pseudogymnoascus destructans (Pd), and addresses specific research priorities identified by partners in conjunction with the White-Nose Syndrome National Plan.
During annual bat population surveys, participating agencies collect swabs of bat skin, guano, hibernaculum sediment, and environmental substrate. If clinical signs of white-nose syndrome (WNS) are observed in the population, carcasses or wing biopsies from affected bats are collected for diagnostic testing.
Preliminary characterization of risk factors associated with Pd movement suggest that new detections are related to the distance to nearest known Pd-positive sites and only mildly associated with the size of a site’s hibernating bat population. We are also investigating use of community guano samples from summer roosts for Pd surveillance. This strategy has particularly utility for areas where bat hibernation sites are not known or are difficult to access.
In 2012 NWHC scientists collected swabs from bat wings, cave walls, and equipment used in and near study sites in Kentucky, Indiana, Ohio, Tennessee, and Virginia. They also collected guano from individual bats and sediment from the floor of underground summer roost sites. NWHC scientists found that bats occupying hibernation sites in summer can harbor Pd on their skin, and that Pd is more readily detectable in their guano. More findings are summarized in a USGS News Release: Deadly fungus affecting hibernating bats could spread during summer.
Despite active national surveillance efforts to detect the spread of WNS, the 2016 detection of WNS in Washington State illustrates the ongoing importance of investigating wildlife mortality events as part of a comprehensive wildlife disease surveillance strategy, and wildlife managers are encouraged to report unusual bat mortality or bats displaying clinical signs suggestive of WNS to the NWHC for further investigation. NWHC can also answer questions about designing WNS surveillance and response plans relevant to your state and help with testing samples collected as part of opportunistic or targeted surveillance efforts in accordance with the national Pd surveillance strategy.
If you are a private individual or entity with a question about white-nose syndrome in your area, please contact your state department of natural resources or state agency of game and fish. A list of State and Federal agencies can be found at fishwildlife.org.
If you are from a State, Federal, or Tribal agency, please see more information on reporting mortality events and submitting samples related to white-nose syndrome. Tribal, State, and Federal agencies with questions about ongoing surveillance efforts, or who may wish to participate, should contact nwhc-epi@usgs.gov.
More resources on white-nose syndrome surveillance:
- NWHC Bat White-Nose Syndrome/Pd Surveillance Submission Guidelines
- A National Plan for Assisting States, Federal Agencies, and Tribes in Managing White-Nose Syndrome in Bats
- White-Nose Syndrome.org - A Coordinated Response to the Devastating Bat Disease
- White-nose Syndrome Occurrence Map
- White-nose Syndrome Case Definitions
- Biosafety Guidance for Handling and Storage of Pseudogymnoascus destructans
- USFWS Surveillance and Diagnostics Working Group
White-nose syndrome surveillance training videos
Investigating coronaviruses and white-nose syndrome fungus in free-ranging North American bats
White-nose syndrome diagnostic harmonization
White-Nose Syndrome
Below are photos related to white-nose syndrome surveillance.
More photos related to white-nose syndrome are available in the WNS Image Gallery.

Long-wave ultraviolet (UV) light can be used to detect orange fluorescence associated with white-nose syndrome (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome-surveillance) on bat skin.
Long-wave ultraviolet (UV) light can be used to detect orange fluorescence associated with white-nose syndrome (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome-surveillance) on bat skin.

Environmental samples collected in or near bat roosting locations can be used to supplement samples collected from bats for surveillance for white-nose syndrome (WNS) (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome).
Environmental samples collected in or near bat roosting locations can be used to supplement samples collected from bats for surveillance for white-nose syndrome (WNS) (https://www.usgs.gov/centers/nwhc/science/white-nose-syndrome).

This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.
This video shows the proper technique for collecting a skin swab from a bat that is either roosting in place or is manually restrained for white-nose syndrome surveillance.

This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.
This video shows the proper procedure for performing cervical dislocation on a bat with clinical signs of white-nose syndrome to be submitted to a diagnostic laboratory for evaluation.

This video demonstrates the proper procedure for collecting a skin biopsy from a bat when conducting white-nose syndrome surveillance.
This video demonstrates the proper procedure for collecting a skin biopsy from a bat when conducting white-nose syndrome surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.
Tables are placed below bat boxes to collect guano from roosting bats during spring/summer. NWHC is investigating use of community guano samples from summer roosts for Pseudogymnoascus destructans (Pd) surveillance.

Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
Here a scientist is measuring the length of the forearm of a bat captured in Dane County, Wisconsin. General morphometric data are collected from each bat captured.
A little brown bat is being released shortly after samples are collected.
A little brown bat is being released shortly after samples are collected.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
A moist swab is passed over the surface of the forearm and muzzle of each bat. The swab will be analyzed in the lab for the presence of Pseudogymnoascus destructans DNA.
Mist-net and harp trap set up to capture bats for white-nose syndrome surveillance activities. Breckinridge County, KY. July 2012.
Mist-net and harp trap set up to capture bats for white-nose syndrome surveillance activities. Breckinridge County, KY. July 2012.
Scientist taking environmental samples during white-nose syndrome surveillance activities. Breckinridge County, IN. July 2012.
Scientist taking environmental samples during white-nose syndrome surveillance activities. Breckinridge County, IN. July 2012.
Examining the wing of a bat. Monroe County, IN. July 2012.
Examining the wing of a bat. Monroe County, IN. July 2012.
Below are publications about white-nose syndrome.
Determinants of Pseudogymnoascus destructans within bat hibernacula: Implications for surveillance and management of white-nose syndrome
Dispersal hazards of Pseudogymnoascus destructans by bats and human activity at hibernacula in summer
First detection of bat white-nose syndrome in western North America
Optimized methods for total nucleic acid extraction and quantification of the bat white-nose syndrome fungus, Pseudogymnoascus destructans, from swab and environmental samples
A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome
Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructanstructans.
DNA-based detection of the fungal pathogen Geomyces destructans in soil from bat hibernacula
Below are news stories about white-nose syndrome.